Abstract: The present invention relates a promoter capable of strongly expressing a gene linked downstream (3′ side) of the promoter in prokaryote, especially in Escherichia Coli. The promoter of the invention is a promoter for prokaryote having a novel nucleotide sequence between any −35 region sequence and any −10 region sequence. The promoter has a strong promoter activity and thus a target peptide or protein can be produced at a high efficiency and in large amounts through linking the structural gene encoding the target peptide or protein downstream of the promoter.

Abstract: A cysteine-free peptide is produced by producing a fused protein comprising a protein having cysteine at its N-terminal and a cysteine-free peptide ligated to the N-terminal and subsequently subjecting the fused protein to a reaction for cleaving the peptide linkage.

Abstract: A cysteine-free peptide is produced by producing a fused protein comprising a protein having cysteine at its N-terminal and a cysteine-free peptide ligated to the N-terminal and subsequently subjecting the fused protein to a reaction for cleaving the peptide linkage.

Abstract: There are disclosed cloning of an alkaliprotease secretion gene of a mold and a DNA fragment containing a part or all of the alkaliprotease gene. A plasmid into which the DNA fragment is integrated, a transformant obtained by using the plasmid and the production of a useful protein by using the transformant are also disclosed.