Abstract: Provided is a target substance detection device that can detect a target substance accurately and efficiently and can be manufactured at low costs.

Abstract: A detection method including forming a complex by binding, to a target substance, a first substance immobilized to a metal particle with magnetism and a second substance labeled with a fluorescent material, moving the complex by applying a magnetic field, illuminating the complex during movement with excitation light of a predetermined wavelength, the excitation light causing the fluorescent material to emit fluorescence, the fluorescence being enhanced by localized surface plasmon resonance that is produced by the metal particle, capturing the enhanced fluorescence over time and obtaining two-dimensional images, and detecting the target substance in accordance with a light spot included in each of the two-dimensional images, the metal particle including an inner core made of a magnetic material and an outer shell covering the inner core, the outer shell being made of a nonmagnetic metal material that produces the localized surface plasmon resonance.

Abstract: To detect a target substance accurately and effectively, a target substance detection device 1 includes: a liquid-sample introducing plate 2 formed from a light-transmissive plate having a surface on which a liquid sample including a target substance and magnetic particles forming a conjugate with the target substance is introduced, and enabling propagating transmitted light of light irradiated from the rear face upward of the surface as propagated light; a rear face light irradiation unit 3 configured to be able to irradiate the liquid-sample introducing plate 2 with light from the rear face; a first magnetic field application unit 4 disposed on the side of the surface of the liquid-sample introducing plate 2, and configured to apply a magnetic field to move the conjugate in the liquid sample that is introduced on the surface of the liquid-sample introducing plate in the direction away from the liquid-sample introducing plate 2; and an optical-signal detection unit 5 disposed on the side of the surface of th

Abstract: A target substance detection device detecting a target substance using magnetic particles includes a detection chip, a light irradiation unit, and a magnetic field application unit, wherein the detection chip includes a light transmissive member having a sensing surface arranged on a surface constituting a top surface relative to a bottom surface, an inclined surface, and a main body portion capable of receiving light and guiding the light through the interior, the light transmissive member having a light directing structure in which the light applied from the top surface side and passed through the inclined surface is directed via the main body portion to the sensing surface under the condition of total reflection, the light irradiation unit is operable to irradiate the sensing surface with light under the condition of total reflection via the light directing structure, and the magnetic field application unit.

Abstract: The invention relates to a method that detects the target substance by measuring a decrease in the optical signal generated by applying a first magnetic field to move the conjugate in the direction parallel to or away from the surface, etc. The invention also relates to an optical detection device wherein a liquid holding part has a sensing plate that can form a near field on a surface with light irradiated from a side of a rear face under a condition of total reflection, the liquid holding part being capable of holding a liquid sample that has a target substance and a magnetic particle that forms a conjugate with the target substance on the surface of the sensing plate, a light irradiation unit, a light detection unit, and a magnetic field application unit configured to apply a magnetic field to move the conjugate in the direction parallel to or away from the surface, etc.

Abstract: An optical detection method and an optical detection device quickly and accurately detects a micro target substance, such as an antigen, with high sensitivity by using an enhanced electric field. The optical detection device includes: one or more light irradiation units; a detection plate having a laminate structure; a prism in close optical contact to a back surface side of the detection plate and having multiple light incident surfaces with different incidence angles; and a light detection unit which is placed on the front surface side of the detection plate and which detects an optical signal from a sample. Light from the light irradiation unit enters the light incident surfaces of the prism at a fixed angle with respect to the front surface of the detection plate, and the light passing through the prism is irradiated from the back surface side of the detection plate under a total reflection condition.

Abstract: Provided is, among others, a target substance detection device that can be used in detecting a target substance using magnetic particles and that can be manufactured compactly and inexpensively.

Abstract: To detect a target substance accurately and effectively, a target substance detection device 1 includes: a liquid-sample introducing plate 2 formed from a light-transmissive plate having a surface on which a liquid sample including a target substance and magnetic particles forming a conjugate with the target substance is introduced, and enabling propagating transmitted light of light irradiated from the rear face upward of the surface as propagated light; a rear face light irradiation unit 3 configured to be able to irradiate the liquid-sample introducing plate 2 with light from the rear face; a first magnetic field application unit 4 disposed on the side of the surface of the liquid-sample introducing plate 2, and configured to apply a magnetic field to move the conjugate in the liquid sample that is introduced on the surface of the liquid-sample introducing plate in the direction away from the liquid-sample introducing plate 2; and an optical-signal detection unit 5 disposed on the side of the surface of th

Abstract: Provided are an optical detection method and an optical detection device that quickly and accurately detect a micro target substance, such as an antigen, with high sensitivity by using an enhanced electric field.

Abstract: A problem is to provide an optical detection method that enables sensitive and quick detection of a micro target substance in a near field and such a device. As means for solving the problem, an optical detection method for a target substance detects light, such as fluorescence, as an optical signal from a conjugate comprising the target substance on a surface of a sensing plate in a near field, wherein the conjugate comprises at least the target substance bonding with a magnetic particle. The method comprises: detecting the target substance by measuring a decrease in the optical signal generated by applying a first magnetic field to move the conjugate in the direction parallel to or away from the surface, etc.