Patents by Inventor Masayasu Kurono

Masayasu Kurono has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 5552157
    Abstract: For increasing gene entrapping efficiency, it is preferable that liposome membrane to be formed is positively charged. For this purpose, a lipid with quaternary amine has been employed as one of constitutional lipids, but the such lipid may show toxicity to cells to be transformed with the gene for expression. The invention selects, as constitutional lipids for liposomes, N-(.alpha.-trimethylammonioacetyl)-didodecyl-D-glutamate chloride (TMAG), dilauroylphosphatidylcholine (DLPC), and dioleoylphosphatidylethanolamine (DOPE) with molar ratio of 1:2:2, and vortex treatment for preparing the liposomes with multi-layers to entrap the gene therein.
    Type: Grant
    Filed: March 28, 1995
    Date of Patent: September 3, 1996
    Assignee: Kabushiki Kaisha Vitamin Kenkyusya
    Inventors: Kunio Yagi, Hitoshi Noda, Nobuko Ohishi, Masayasu Kurono
  • Patent number: 5530138
    Abstract: There is disclosed a compound shown by a formula of ##STR1## wherein R.sub.1 is a hydrogen atom alkyl group having 1-4 carbon atoms or acyl group having 1-4 carbon atoms: R.sub.2 and R.sub.3 are a hydrogen atom, alkyl group having 1-4 carbon atoms, phenyl radical, halogen atom, cyano radical, acyl group having 1-4 carbon atoms, nitro radical, alkoxy group having 1 or 2 carbon atoms, or substituted or non-substituted amino group, respectively; n is an integer of 1-3; and dotted line means a possible ring, and a salt thereof. The compound and salt bind with muscarinic receptor in brain to develop a powerful actuation thereof and thus those can be used as an effective ingredient for preventing and curing senile dementias, and more particularly Alzheimer's disease.
    Type: Grant
    Filed: September 27, 1994
    Date of Patent: June 25, 1996
    Assignee: Sanwa Kagaku Kenkyusho Co., Ltd.
    Inventors: Yutaka Baba, Tomoo Suzuki, Tsunemasa Suzuki, Kiyotaka Hirooka, Masayasu Kurono, Kiichi Sawai
  • Patent number: 5474908
    Abstract: A method of enzymatically measuring hydrogen peroxide by developing a color with use of 3-methyl-2-benzothiazolinoehydrazone as a coupler and in combination with an oxidative color developing reagent with peroxidase, and a reagent therefor is described. Catalase is added to the 3-methyl-2-benzothiazolinonehydrazone reagent as a stabilizer and the reaction is carried out in the presence of ethylenediaminetetraacetic acid or an analogue thereof. The color developing reaction may be carried out in neutral or weak alkali conditions. A reagent is described which comprises a buffer, 3-methyl-2-benzothiazolinohydrazone, and catalase.
    Type: Grant
    Filed: November 25, 1991
    Date of Patent: December 12, 1995
    Assignee: Sanwa Kagaku Kenkyusho Co., Ltd.
    Inventors: Masayasu Kurono, Shizuo Uno, Osamu Takehiro, Kiichi Sawai
  • Patent number: 5474913
    Abstract: Process for the preparation of motilin-like polypeptide, a recombinant DNA therefor, and a plasmid, in which the recombinant DNA is inserted.
    Type: Grant
    Filed: January 26, 1993
    Date of Patent: December 12, 1995
    Assignee: Sanwa Kagaku Kenkyusho Co., Ltd.
    Inventors: Masayasu Kurono, Takahiko Mitani, Haruo Takahashi, Kenichi Tanaka, Katsuya Fujimura, Kiichi Sawai
  • Patent number: 5468728
    Abstract: A readily absorbable type of motilin preparation containing a motilin-like active substance and a surface active agent is provided, in which the motilin-like active substance is mainly L-leucine-13-motilin-homoserine, and the surface active agent is selected from the group consisting of a bile salt, saponin and a polyethylene glycol higher-alcohol ether for nasal administration purposes and is a peptide lytic enzyme inhibitor for oral administration purposes.
    Type: Grant
    Filed: September 8, 1993
    Date of Patent: November 21, 1995
    Assignee: Sanwa Kagaku Kenkyusho Co., Ltd.
    Inventors: Kiichi Sawai, Masayasu Kurono, Takahiko Mitani, Makoto Sato, Hiroyuki Oowaki, Bunkichi Kato, Haruo Takahashi
  • Patent number: 5459049
    Abstract: A process for the preparation of a polypeptide of the formula ##STR1## wherein A' is Pro, Gly, Asp or Ser; B' is Gly, Asn or Ser; C is Gln, Glu or Asp; D is Asn, Glu or Asp; X' is a residue of amino acid other than Met; E is Gln, Lys or Arg, and Y' is a residue of homoserine or homoserine-lactone, as well as a recombinant DNA and expression plasmid for preparing the polypeptide.
    Type: Grant
    Filed: January 13, 1993
    Date of Patent: October 17, 1995
    Assignee: Sanwa Kagaku Kenkyushko Co., Ltd.
    Inventors: Masayasu Kurono, Takahiko Matani, Haruo Takahashi, Kenichi Tanaka, Katsuya Fujimura, Yuji Hayashi, Yohei Kobayashi, Kiichi Sawai
  • Patent number: 5447946
    Abstract: Hydantoin derivatives and salts thereof, intermediates therefor, process for the preparation thereof, and medicines containing the derivative, wherein said derivatives have the formula ##STR1## wherein one of V and W is hydrogen and the other is a halogenomethyl group, 1H-tetrazol-5-yl radical, --COOR group,in which R is hydrogen atom, an alkyl group, --(CH.sub.2 CH.sub.2 O)nCH.sub.3 group (n is an integer of 1 to 113) or substituted phenyl, ##STR2## in which R.sub.1 and R.sub.2 are same or different independently, each is hydrogen atom, an alkyl group, substituted phenyl or --(CH.sub.2 CH.sub.2 O)nCH.sub.3 group (n has the meaning as referred to) or R.sub.1 may form a heterocyclic ring together with R.sub.2 and nitogen or oxygen atom,--CH.sub.2 OH.sub.3 group or ##STR3## group, in which R.sub.3 and R.sub.
    Type: Grant
    Filed: September 23, 1994
    Date of Patent: September 5, 1995
    Assignee: Sanwa Kagaku Kenkyusho Co., Ltd.
    Inventors: Masayasu Kurono, Yasuaki Kondo, Takuji Yamaguchi, Kenji Miura, Toshinao Usui, Naofumi Terada, Kyoichi Asano, Kuniharu Mizuno, Akira Matsubara, Noriaki Kato, Kiichi Sawai, Ryoichi Unno, Hiroshi Ozawa, Masato Fukushima
  • Patent number: 5434165
    Abstract: An aromatic amino-substituted compound represented by formula: ##STR1## wherein A represents CH, N, or N.fwdarw.O; R.sub.1 represents a nitro group or an amino group; R.sub.2 represents a hydrogen atom, a lower alkyl group, or an acyl group; and R.sub.3 represents a group: ##STR2## wherein m represents 0 or 1; n represents an integer of from 0 to 3; R.sub.4 and R.sub.5 each represents a hydrogen atom or a lower alkyl group; R.sub.6 and R.sub.7 each represents a hydrogen atom or a straight chain or branched lower alkyl group; R.sub.4 and R.sub.6 may be joined together to form an alkylene chain forming a heterocyclic ring; R.sub.5 and R.sub.7 may be joined together to form an alkylene chain forming a heterocyclic ring and R.sub.6 and R.sub.
    Type: Grant
    Filed: November 13, 1992
    Date of Patent: July 18, 1995
    Assignee: Sanawa Kagaku Kenkyusho Company, Ltd.
    Inventors: Masayasu Kurono, Yutaka Baba, Tomoo Suzuki, Tsunemasa Suzuki, Kiyotaka Hirooka, Kiichi Sawai
  • Patent number: 5434082
    Abstract: Early diagnosis of mastitis or garget is carried out by measuring the concentration of 3-hydroxybutyric acid in milk. Used to this end is a reagent reacting with 3-hydroxybutyric acid to show a color at a concentration of 100 .mu.mol/l or higher.
    Type: Grant
    Filed: January 7, 1993
    Date of Patent: July 18, 1995
    Assignee: Sanwa Kagaku Kenkyusho Co., Ltd
    Inventors: Hideo Yamamoto, Eiji Furukawa, Hiromoto Asai, Masayasu Kurono, Kiichi Sawai
  • Patent number: 5432261
    Abstract: A pharmaceutical composition for stimulating peristaltic contraction in the intestinal canal which contains a pharmaceutically acceptable carrier or excipient and an effective amount of a polypeptide, or a salt thereof, having a pharmacological activity similar to motilin. The polypeptide has the formula B.sub.1 -Z.sub.1 -A.sub.1 -Z.sub.2 -B.sub.2 -Thr-B.sub.3 -A.sub.2 -Glu-Z.sub.3 -C-Arg-X'-Gln-Glu-Lys-Glu-Arg-D-Lys-Gly-E-Y, with Z.sub.1,Z.sub.2 and Z.sub.3 each being an amino acid residue having a hydrophobic side-chain, A.sub.1 and A.sub.2 each being an amino acid residue such as Pro, Gly, Asn and Ser, B.sub.1, B.sub.2 and B.sub.
    Type: Grant
    Filed: September 11, 1992
    Date of Patent: July 11, 1995
    Assignee: Sanwa Kagaku Kenkyusho Co. Ltd.
    Inventors: Masayasu Kurono, Takahiko Mitani, Haruo Takahashi, Kenichi Tanaka, Katsuya Fujimura, Yuji Hayashi, Yohei Kobayashi, Kiichi Sawai
  • Patent number: 5428015
    Abstract: Vasoactive intestinal polypeptide (VIP) analogues and use thereof are disclosed. The VIP analogues are shown by the formula ofHis-Ser-Asp-Ala-Val-Phe-Thr-Asp-Asn-Tyr-Thr-Arg-Leu-Arg-Lys-Gln-X-Ala-Val-L ys-Lys-Tyr-Leu-Asn-Ser-Ile-Leu-Asn-Ywherein X is a residue of amino acid other than methionine (Met); and Y is homoserine, homoserine-lactone, amidized homoserine, a residue reacted homoserine-lactone with a primary alkyl amine having carbon atoms not exceeding 20, or an optional polypeptide chain and containing an amidized homoserine at C-terminal.Each of the VIP analogues is employed as an effective ingredient for various medicines and a hair tonic, and more particularly curing an asthma and impotence.
    Type: Grant
    Filed: February 24, 1994
    Date of Patent: June 27, 1995
    Assignee: Sana Kagaku Kenkyusho Co., Ltd.
    Inventors: Masayasu Kurono, Takahiko Mitani, Haruo Takahashi, Kiichi Sawai
  • Patent number: 5376637
    Abstract: There is disclosed a pharmaceutical preparation which contains vasoactive intestinal polypeptide (VIP), its analogue or a salt thereof, and a surfactant. The preparation shall contains further a peptidase inhibitor. The composition is administrated to suppress allergies, reduce blood pressure, increase secretion of tear, cure asthma and impotence and others.
    Type: Grant
    Filed: April 16, 1992
    Date of Patent: December 27, 1994
    Assignee: Sanwa Kagaku Kenkyusho Co., Ltd.
    Inventors: Kiichi Sawai, Masayasu Kurono, Takahiko Mitani, Makoto Sato, Haruo Takahashi, Hiroyuki Ohwaki
  • Patent number: 5364533
    Abstract: A process for separating and recovering serum and plasma components from a whole blood sample including passing a whole blood sample through a separating filter formed of a blood cell separating layer composed mainly of fibers impregnated with a coating agent. A device includes a blood collector having a needle at one end and a blood suction means at the other end, and a separating filter including a blood cell separating layer, the filter being provided in the intermediate zone of the blood collector, whereby a whole blood sample sucked through the needle by depressurizing the blood suction means is passed through the separating filter.
    Type: Grant
    Filed: July 14, 1992
    Date of Patent: November 15, 1994
    Assignee: Sanwa Kagaku Kenkyusho Co., Ltd.
    Inventors: Shinji Ogura, Hiroshi Okada, Shizuo Uno, Takashi Iida, Hiromoto Asai, Masayasu Kurono, Kiichi Sawai
  • Patent number: 5356880
    Abstract: The invention provides a novel glycoside which contains as the aglycon a 4-(substituted phenyl)piperazine-1-yl derivative of glycyrrhetinic acid and 11-deoxo-glycyrrhetinic acid or their derivative as well as a composition for the treatment of virus infection, which contains these compounds as a main active component.
    Type: Grant
    Filed: May 28, 1992
    Date of Patent: October 18, 1994
    Assignee: Sanwa Kagaku Kenkyusho Co., Ltd.
    Inventors: Masayasu Kurono, Yoshiro Ishiwata, Syoji Yokochi, Kyoichi Asano, Takahiko Mitani, Takuji Kakigami, Noriyuki Iwata, Kougaku Isogawa, Yutaka Baba, Hiroyuki Ohwaki, Kiichi Sawai, Hiromoto Kimura, Masato Fukushima, Ryoichi Unno, Tamaki Ohtuka
  • Patent number: 5340806
    Abstract: A composition comprising an organogermanium compound represented by the formula ##STR1## wherein n is an integer of 1 or more, R is hydrogen, alkyl, --COOH, --COOR', phenyl, ##STR2## and R' is a lower alkyl and a high molecular carrier, as well as an immunity adjusting agent comprising the composition. The treatment of hepatitis with compositions containing this germanium derivative.
    Type: Grant
    Filed: January 7, 1993
    Date of Patent: August 23, 1994
    Assignee: Sanwa Kagaku Kenkyusho Co., Ltd.
    Inventors: Kiichi Sawai, Masayasu Kurono, Juichi Awaya, Akio Kojima, Hideaki Ninomiya, Yoshiro Ishiwata, Masahiro Nakajima
  • Patent number: 5336688
    Abstract: A composition comprising an organogermanium compound represented by the formula ##STR1## wherein n is an integer of 1 or more, R is hydrogen, alkyl, --COOH, --COOR', phenyl, ##STR2## and R' is a lower alkyl and a high molecular carrier, as well as an immunity adjusting agent comprising the composition.
    Type: Grant
    Filed: January 25, 1993
    Date of Patent: August 9, 1994
    Assignee: Sanwa Kagaku Kenkyusho Co., Ltd.
    Inventors: Kiichi Sawai, Masayasu Kurono, Juichi Awaya, Akio Kojima, Hideako Ninomiya, Yoshiro Ishiwata, Masahiro Nakajima
  • Patent number: 5308836
    Abstract: There is disclosed a pharmaceutical composition of stabilized [Leu.sup.13 ]-motilin-Hse. The composition comprises at least one of organic acids and salts thereof, as a first stabilizer, and pH thereof is adjusted in a range of 5.5-8.0. The composition may contain at least one of substances selected from saccharides, amino acids, proteins and salts thereof, as a second stabilizer. Conversion of [Leu.sup.13 ]-motilin-Hse into [Leu.sup.13 ]-motilin-Hse-lactone is suppressed by increasing pH value and deamidization in asparagine residue at 19-position is suppressed by the presence of the organic acid or salt thereof.
    Type: Grant
    Filed: September 7, 1993
    Date of Patent: May 3, 1994
    Assignee: Sanwa Kagaku Kenkyusho Co., Ltd.
    Inventors: Kiichi Sawai, Masayasu Kurono, Yasuaki Kondo, Makoto Sato, Toshiyuki Kouzaki
  • Patent number: 5290679
    Abstract: Even when the specimen contains human granulocyte elastase in the form of a mixture of free elastase with an elastase-inhibitor complex or complexes, the present invention enables the total quantity of elastase in that specimen to be precisely detected. The inhibitor is added to free elastase to convert it into an elastase-inhibitor complex, whereby the quantity of elastase can be measured by immunoassay as the total amount including the previously existing elastase-inhibitor complex. It is possible to precisely measure the total amount of elastase in mucus collected from the cervical canal of a pregnant woman, sputum or a rinsed solution of bronchovesicular lavage in which free elastase is mixed with an elastase-inhibitor complex.
    Type: Grant
    Filed: June 15, 1992
    Date of Patent: March 1, 1994
    Assignee: Sanwa Kagaku Kenkyusho Co., Ltd.
    Inventors: Toshihiko Terao, Naohiro Kanayama, Akihiro Morioka, Yoshika Yasuda, Masami Kamiya, Juichi Awaya, Masayasu Kurono, Kiichi Sawai
  • Patent number: 5279835
    Abstract: A 3-oxygermylpropionic acid preparation is provided, which activates the action of 3-oxygermylpropionic acid having the general formula:[(O.sub.1/2).sub.3 GeCH.sub.2 CH.sub.2 COOH]n wherein n=an integer of at least 1 and is readily solubilized and disintegrated in vivo. The present preparation is manufactured by compression-molding at high pressure a composition comprising 3-oxygermylpropionic acid and two or more low-molecular substance of high solubility in water. The low-molecular substances are selected from the group consisting of white sugar, sorbitol, fructose, sucrose, glucose, lactose, mannitol and sodium chloride.
    Type: Grant
    Filed: June 30, 1992
    Date of Patent: January 18, 1994
    Assignee: Sanwa Kagaku Kenkyusho Co. Ltd.
    Inventors: Kiichi Sawai, Masayasu Kurono, Takahiko Mitani, Yasuaki Kondo, Makoto Sato, Yoshiro Ishiwata, Syoji Yokochi, Toshiyuki Kouzaki
  • Patent number: 5260056
    Abstract: An organic germanium compound to be effectively used for curing AIDS or making AIDS asymtomatic in various forms is expressed by the following rational formula: ##STR1## wherein n is an integer of 1 or 2 or more, is in the form of a white acicular or needle crystal, shows a solubility of 1.570/100 ml in water at 25.degree. C. and exhibits a melting (decomposition) point of 240.degree. C.
    Type: Grant
    Filed: January 14, 1991
    Date of Patent: November 9, 1993
    Assignee: Sanwa Kagaku Kenkyusho Co. Ltd.
    Inventors: Kiichi Sawai, Masayasu Kurono, Takahiko Mitani, Naohisa Ninomiya, Yoshiro Ishiwata, Syoji Yokochi, Kyoichi Asano, Kaneo Yamada, Masashi Taki, Takashi Meguro, Mikio Minamitani, Takao Matumoto, Yuichi Shiokawa