Patents by Inventor Masayo Date
Masayo Date has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11859205Abstract: Culture media, which contain albumin carrying a reduced amount of fatty acid, are useful for culturing stem cells.Type: GrantFiled: June 11, 2020Date of Patent: January 2, 2024Assignee: AJINOMOTO CO., INC.Inventors: Yoko Kuriyama, Sho Senda, Yumi Ando, Tomomi Yoshida, Haruna Sato, Daisuke Ejima, Takayoshi Fujii, Masayo Date, Manabu Kitazawa
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Publication number: 20210253629Abstract: The present invention provides a method for producing a refolded protein, the method comprising a step for mixing, inside a micro mixer, a buffer and a solubilization solution for a protein that has lost higher order structure or that has become insoluble.Type: ApplicationFiled: May 5, 2021Publication date: August 19, 2021Applicant: AJINOMOTO CO., INC.Inventors: Daisuke EJIMA, Yuichi NAKAHARA, Keisuke KATO, Masayo DATE
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Publication number: 20200370006Abstract: Culture media, which contain albumin carrying a reduced amount of fatty acid, are useful for culturing stem cells.Type: ApplicationFiled: June 11, 2020Publication date: November 26, 2020Applicant: AJINOMOTO CO., INC.Inventors: Yoko KURIYAMA, Sho SENDA, Yumi ANDO, Tomomi YOSHIDA, Haruna SATO, Daisuke EJIMA, Takayoshi FUJII, Masayo DATE, Manabu KITAZAWA
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Patent number: 10463594Abstract: A method for perming or coloring hair, in which hair is treated with a cosmetic composition including a protein-glutaminase and not including an alkaline substance. A method of enhancing hair-permeation of an active ingredient included in a cosmetic composition, in which a protein glutaminase is included in the cosmetic composition. The cosmetic composition does not include an alkaline substance, and the active ingredient is at least one of a hair perm agent and a hair coloring agent.Type: GrantFiled: January 5, 2017Date of Patent: November 5, 2019Assignee: AJINOMOTO CO., INC.Inventors: Keiichi Yokoyama, Eiko Oshimura, Masayo Date, Yukiko Umezawa
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Patent number: 10308969Abstract: The present invention provides a method for producing a multimeric protein composed of a monomeric protein, wherein the monomeric protein is obtained by fusing a protein having an immunoglobulin fold structure to a protein that can serve as a subunit structure, the method including the steps of: (A) preparing the monomeric protein having an insoluble granular form in cells of a microorganism; (B) solubilizing the monomeric protein prepared in step (A) with an aqueous solution containing lauroyl-L-Glu; (C) diluting a solution obtained in step (B) in a buffer containing arginine hydrochloride to lower a concentration of lauroyl-L-Glu; and (D) replacing a solvent of a solution obtained in step (C) with a buffer using gel filtration chromatography or the like.Type: GrantFiled: December 12, 2013Date of Patent: June 4, 2019Assignee: AJINOMOTO CO., INC.Inventors: Daisuke Ejima, Haruna Sato, Kouhei Tsumoto, Masayo Date
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Publication number: 20170112751Abstract: A method for perming or coloring hair, in which hair is treated with a cosmetic composition including a protein-glutaminase and not including an alkaline substance. A method of enhancing hair-permeation of an active ingredient included in a cosmetic composition, in which a protein glutaminase is included in the cosmetic composition. The cosmetic composition does not include an alkaline substance, and the active ingredient is at least one of a hair perm agent and a hair coloring agent.Type: ApplicationFiled: January 5, 2017Publication date: April 27, 2017Applicant: AJINOMOTO CO., INC.Inventors: Keiichi YOKOYAMA, Eiko OSHIMURA, Masayo DATE, Yukiko UMEZAWA
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Publication number: 20160075993Abstract: Culture media, which contain albumin carrying a reduced amount of fatty acid, are useful for culturing stem cells.Type: ApplicationFiled: November 27, 2015Publication date: March 17, 2016Applicant: AJINOMOTO CO., INC.Inventors: Yoko KURIYAMA, Shou SENDA, Yumi ANDO, Tomomi YOSHIDA, Haruna SATO, Daisuke EJIMA, Takayoshi FUJII, Masayo DATE, Manabu KITAZAWA
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Publication number: 20150290108Abstract: The problem of the present invention is to provide a protein hydrophilicity-increasing agent capable of effectively increasing water permeability of (swelling) hair, skin and the like while suppressing damages on the hair, skin and the like constituted of the protein. The present invention solves the problem by providing a protein hydrophilicity-increasing agent containing a protein-glutaminase, and a protein hydrophilicity-increasing agent containing both a protein-glutaminase and transglutaminase.Type: ApplicationFiled: April 16, 2015Publication date: October 15, 2015Applicant: AJINOMOTO CO., INC.Inventors: KEIICHI YOKOYAMA, EIKO OSHIMURA, MASAYO DATE, YUKIKO UMEZAWA
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Patent number: 9062291Abstract: A transglutaminase protein which is mutated to have improved heat resistance and/or pH stability. A mutation is introduced into WT transglutaminase at a cysteine residue capable of forming a disulfide bond (SS bond).Type: GrantFiled: August 14, 2009Date of Patent: June 23, 2015Assignee: AJINOMOTO CO., INC.Inventors: Keiichi Yokoyama, Mototaka Suzuki, Tatsuki Kashiwagi, Eiichiro Suzuki, Masayo Date, Seiichi Taguchi
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Publication number: 20140099672Abstract: The present invention provides a method for producing a multimeric protein composed of a monomeric protein, wherein the monomeric protein is obtained by fusing a protein having an immunoglobulin fold structure to a protein that can serve as a subunit structure, the method including the steps of: (A) preparing the monomeric protein having an insoluble granular form in cells of a microorganism; (B) solubilizing the monomeric protein prepared in step (A) with an aqueous solution containing lauroyl-L-Glu; (C) diluting a solution obtained in step (B) in a buffer containing arginine hydrochloride to lower a concentration of lauroyl-L-Glu; and (D) replacing a solvent of a solution obtained in step (C) with a buffer using gel filtration chromatography or the like.Type: ApplicationFiled: December 12, 2013Publication date: April 10, 2014Applicant: Ajinomoto Co., Inc.Inventors: Daisuke Ejima, Haruna Sato, Kouhei Tsumoto, Masayo Date
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Patent number: 8597907Abstract: The present invention provides a method for efficiently producing an industrially useful protein in coryneform bacteria, and more particularly, a method for efficiently producing a protein for which secretion was difficult using conventional protein secretion pathways. In particular, the present invention provides a method for efficiently producing heterologous proteins comprising culturing coryneform bacteria containing an genetic construction containing a promoter sequence which functions in coryneform bacteria, a nucleic acid sequence encoding a Tat system-dependent signal peptide region, and a nucleic acid sequence encoding a heterologous protein, in the direction from 5?-end to 3?-end, and secretory producing the heterologous protein by coryneform bacteria.Type: GrantFiled: October 19, 2006Date of Patent: December 3, 2013Assignee: Ajinomoto Co., Inc.Inventors: Masayo Date, Yoshimi Kikuchi, Hiroshi Itaya, Nami Nakamura
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Patent number: 8580537Abstract: The present invention provides transglutaminases with improved heat resistance. Specifically, the present invention provides mutant transglutaminase proteins with improved heat resistance as obtained by introducing appropriate mutations into transglutaminases, which results in the incorporation of a disulfide bond.Type: GrantFiled: September 6, 2011Date of Patent: November 12, 2013Assignee: Ajinomoto Co., Inc.Inventors: Mototaka Suzuki, Masayo Date, Keiichi Yokoyama
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Patent number: 8187835Abstract: By culturing a methanol-assimilating bacterium which harbors a DNA construct which contains a promoter sequence that functions in the methanol-assimilating bacterium and a nucleotide sequence that encodes a polypeptide containing a signal sequence and an objective protein which is functionally connected to the promoter sequence, in a liquid medium containing methanol as a major carbon source, the bacterium is allowed to secrete the objective protein, and the secreted objective protein is recovered.Type: GrantFiled: November 13, 2007Date of Patent: May 29, 2012Assignee: Ajinomoto Co., Inc.Inventors: Hiroshi Itaya, Yoshimi Kikuchi, Masayo Date
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Patent number: 8105802Abstract: The present invention provides a neutral metalloprotease from actinomycetes which selectively cleaves a pro-structure part of a microbial protransglutaminase and a gene encoding said neutral metalloprotease. An active microbial transglutaminase having the pro-structure part cleaved can be obtained by culturing a microorganism into which a gene encoding the neutral metalloprotease from actinomycetes according to the present invention has been introduced, where by producing the neutral metalloprotease from actinomycetes, and reacting it on a microbial protransglutaminase.Type: GrantFiled: March 1, 2010Date of Patent: January 31, 2012Assignee: Ajinomoto Co., Inc.Inventors: Yukiko Umezawa, Keiichi Yokoyama, Yoshimi Kikuchi, Masayo Date, Norimasa Onishi
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Publication number: 20120021458Abstract: The present invention provides transglutaminases with improved heat resistance. Specifically, the present invention provides mutant transglutaminase proteins with improved heat resistance as obtained by introducing appropriate mutations into transglutaminases, which results in the incorporation of a disulfide bond.Type: ApplicationFiled: September 6, 2011Publication date: January 26, 2012Inventors: Mototaka Suzuki, Masayo Date, Keiichi Yokoyama
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Patent number: 7972829Abstract: The present invention relates to a process for secretory production of a foreign protein, in particular, transglutaminase by a coryneform bacterium. According to the present invention, a process is provided for the secretory production of a foreign protein, in particular, transglutaminase, by making a coryneform bacterium to produce an industrially useful foreign protein, in particular, transglutaminase and efficiently release the product extracellularly (i.e., secretory production).Type: GrantFiled: February 26, 2010Date of Patent: July 5, 2011Assignee: Ajinomoto Co., Inc.Inventors: Yoshimi Kikuchi, Masayo Date, Yukiko Umezawa, Keiichi Yokoyama, Hiroshi Matsui
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Publication number: 20100297729Abstract: The present invention relates to a process for secretory production of a foreign protein, in particular, transglutaminase by a coryneform bacterium. According to the present invention, a process is provided for the secretory production of a foreign protein, in particular, transglutaminase, by making a coryneform bacterium to produce an industrially useful foreign protein, in particular, transglutaminase and efficiently release the product extracellularly (i.e., secretory production).Type: ApplicationFiled: February 26, 2010Publication date: November 25, 2010Applicant: AJINOMOTO CO., INCInventors: Yoshimi Kikuchi, Masayo Date, Yukiko Umezawa, Keiichi Yokoyama, Hiroshi Matsui
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Publication number: 20100159560Abstract: The present invention provides a neutral metalloprotease from actinomycetes which selectively cleaves a pro-structure part of a microbial protransglutaminase and a gene encoding said neutral metalloprotease. An active microbial transglutaminase having the pro-structure part cleaved can be obtained by culturing a microorganism into which a gene encoding the neutral metalloprotease from actinomycetes according to the present invention has been introduced, where by producing the neutral metalloprotease from actinomycetes, and reacting it on a microbial protransglutaminase.Type: ApplicationFiled: March 1, 2010Publication date: June 24, 2010Inventors: Yukiko Umezawa, Keiichi Yokoyama, Yoshimi Kikuchi, Masayo Date, Norimasa Onishi
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Publication number: 20100143970Abstract: A transglutaminase protein which is mutated to have improved heat resistance and/or pH stability. A mutation is introduced into WT transglutaminase at a cysteine residue capable of forming a disulfide bond (SS bond).Type: ApplicationFiled: August 14, 2009Publication date: June 10, 2010Inventors: Keiichi Yokoyama, Mototaka Suzuki, Tatsuki Kashiwagi, Eiichiro Suzuki, Masayo Date, Seiichi Taguchi
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Patent number: 7723067Abstract: The present invention relates to a process for secretory production of a foreign protein, in particular, transglutaminase by a coryneform bacterium. According to the present invention, a process is provided for the secretory production of a foreign protein, in particular, transglutaminase, by making a coryneform bacterium to produce an industrially useful foreign protein, in particular, transglutaminase and efficiently release the product extracellularly (i.e., secretory production).Type: GrantFiled: April 1, 2002Date of Patent: May 25, 2010Assignee: Ajinomoto Co., Inc.Inventors: Yoshimi Kikuchi, Masayo Date, Yukiko Umezawa, Keiichi Yokoyama, Hiroshi Matsui