Patents by Inventor Masayoshi Itoh
Masayoshi Itoh has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11839697Abstract: Provided is a means for identifying cells suitable for transplantation into a cornea, or a means for producing a cell population suitable for transplantation into a cornea using the same. A method for producing a cell population suitable for transplantation into a cornea, the method comprising the steps of (1) preparing a cell population cultured under conditions suitable for inducing differentiation into corneal endothelial cells, and (2) measuring the expression of at least one gene selected from the group consisting of POU6F2, LMX1B, and TFAP2B in the cell population.Type: GrantFiled: June 19, 2018Date of Patent: December 12, 2023Assignee: OSAKA UNIVERSITYInventors: Kohji Nishida, Motokazu Tsujikawa, Susumu Hara, Satoshi Kawasaki, Masahito Yoshihara, Masayoshi Itoh, Hideya Kawaji
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Patent number: 10968428Abstract: A molecular marker expressed specifically in corneal endothelial cells, and a method for producing one or more corneal endothelial cells using the marker and a method for evaluating one or more corneal endothelial cells using the marker are provided. At least one molecule selected from the group consisting of ZP4, MRGPRX3, GRIP1, GLP1R, HTR1D, and CLRN1 is used as a marker specific to corneal endothelial cells.Type: GrantFiled: March 3, 2017Date of Patent: April 6, 2021Assignee: OSAKA UNIVERSITYInventors: Kohji Nishida, Motokazu Tsujikawa, Susumu Hara, Satoshi Kawasaki, Masahito Yoshihara, Masayoshi Itoh, Hideya Kawaji, Hiroko Ohmiya
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Publication number: 20200101200Abstract: Provided is a means for identifying cells suitable for transplantation into a cornea, or a means for producing a cell population suitable for transplantation into a cornea using the same. A method for producing a cell population suitable for transplantation into a cornea, the method comprising the steps of (1) preparing a cell population cultured under conditions suitable for inducing differentiation into corneal endothelial cells, and (2) measuring the expression of at least one gene selected from the group consisting of POU6F2, LMX1B, and TFAP2B in the cell population.Type: ApplicationFiled: June 19, 2018Publication date: April 2, 2020Inventors: Kohji Nishida, Motokazu Tsujikawa, Susumu Hara, Satoshi Kawasaki, Masahito Yoshihara, Masayoshi Itoh, Hideya Kawaji
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Patent number: 10066264Abstract: The present invention is to provide a method for analyzing a target nucleic acid, by which the target nucleic acid can be analyzed rapidly and easily. In order to achieve the above object, the present invention provides a method for analyzing a target nucleic acid in a sample, including the step of: analyzing the target nucleic acid in the sample by bringing the sample into contact with a label and with a primer or probe that can hybridize to the target nucleic acid. The primer or probe is immobilized on a solid phase. The label does not emit light when the primer or probe does not hybridize to the target nucleic acid, whereas the label emits light when the primer or probe has hybridized to the target nucleic acid. The analysis is carried out by detecting the light emitted from the label.Type: GrantFiled: August 29, 2013Date of Patent: September 4, 2018Assignee: KABUSHIKI KAISHA DNAFORMInventors: Yoshihide Hayashizaki, Masayoshi Itoh, Takahiro Arakawa, Kengo Usui, Sotaro Uemura, Yasumasa Mitani
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Publication number: 20170253855Abstract: A molecular marker expressed specifically in corneal endothelial cells, and a method for producing one or more corneal endothelial cells using the marker and a method for evaluating one or more corneal endothelial cells using the marker are provided. At least one molecule selected from the group consisting of ZP4, MRGPRX3, GRIP1, GLP1R, HTR1D, and CLRN1 is used as a marker specific to corneal endothelial cells.Type: ApplicationFiled: September 4, 2015Publication date: September 7, 2017Applicant: OSAKA UNIVERSITYInventors: Kohji NISHIDA, Motokazu TSUJIKAWA, Susumu HARA, Satoshi KAWASAKI, Masahito YOSHIHARA, Masayoshi ITOH, Hideya KAWAJI, Hiroko OHMIYA
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Publication number: 20150152496Abstract: The present invention is to provide a method for analyzing a target nucleic acid, by which the target nucleic acid can be analyzed rapidly and easily. In order to achieve the above object, the present invention provides a method for analyzing a target nucleic acid in a sample, including the step of: analyzing the target nucleic acid in the sample by bringing the sample into contact with a label and with a primer or probe that can hybridize to the target nucleic acid. The primer or probe is immobilized on a solid phase. The label does not emit light when the primer or probe does not hybridize to the target nucleic acid, whereas the label emits light when the primer or probe has hybridized to the target nucleic acid. The analysis is carried out by detecting the light emitted from the label.Type: ApplicationFiled: August 29, 2013Publication date: June 4, 2015Inventors: Yoshihide Hayashizaki, Masayoshi Itoh, Takahiro Arakawa, Kengo Usui, Sotaro Uemura, Yasumasa Mitani
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Publication number: 20110236900Abstract: A method for determining the presence or absence of a mutation on the basis of the presence or absence of amplification with high reliability is provided. A target sequence including a target site contained in a sample nucleic acid is amplified using a primer that can hybridize to a region including the target site contained in the sample nucleic acid in the presence of a novel MutS having an amino acid sequence of SEQ ID NO: 2, and then the presence or absence of a mutation at the target site is determined on the basis of the presence or absence of amplification. The novel MutS binds more specifically to a mismatched base pair than to a fully-matched base pair, whereby an extension reaction caused by a mismatch-binding primer is suppressed. Thus, according to the present invention, the presence or absence of a mutation can be determined with high reliability.Type: ApplicationFiled: November 27, 2009Publication date: September 29, 2011Applicants: RIKEN, KABUSHIKI KAISHA DNAFORMInventors: Yoshihide Hayashizaki, Masayoshi Itoh, Hajime Kanamori, Kengo Usui, Kazuhito Nomura
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Publication number: 20100221787Abstract: A DNA polymerase suitable for specific isothermal amplification methods and an isothermal amplification method using the DNA polymerase are provided. In the presence of a DNA polymerase including a protein described in the following item (a) or (b), an amplification reaction of a target nucleic acid sequence in a nucleic acid sample is carried out isothermally using a first primer shown in the following (X). By using the DNA polymerase, it becomes possible to carry out the amplification reaction using the primer within a shorter time than ever before. (a) a protein having an amino acid sequence represented by SEQ ID NO. 23 (b) a protein having an amino acid sequence represented by SEQ ID NO.Type: ApplicationFiled: October 24, 2008Publication date: September 2, 2010Applicants: RIKEN, KABUSHIKI KAISHA DNAFORMInventors: Yoshihide Hayashizaki, Masayoshi Itoh, Alexander Lezhava, Yoshimi Benno, Yasumasa Mitani, Hajime Kanamori
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Publication number: 20100047862Abstract: This invention provides a novel DNA polymerase obtained from Bacillus smithii JCM9076, which has novel features in terms of, for example, optimal reaction conditions (e.g., optimal temperature) and enzyme activity. More particularly, a novel DNA polymerase is a pol I type DNA polymerase, which is any of proteins (a) to (f) below and has DNA polymerase activity: (a) a protein comprising the amino acid sequence as shown in SEQ ID NO: 7; (b) a protein consisting of an amino acid sequence derived from the amino acid sequence as shown in SEQ ID NO: 7 by deletion, substitution, or addition of one or several amino acid residues; (c) a protein consisting of the amino acid sequence as shown in SEQ ID NO: 9; and (d) a protein consisting of an amino acid sequence derived from the amino acid sequence as shown in SEQ ID NO: 9 by deletion, substitution, or addition of one or several amino acid residues.Type: ApplicationFiled: October 20, 2006Publication date: February 25, 2010Inventors: Yoshihide Hayashizaki, Masayoshi Itoh, Yoshimi Benno, Alexander Lezhava
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Publication number: 20100035249Abstract: The present invention provides methods for the sequencing of all RNA species within an RNA sample, such as the RNA content obtained from a cell, a tissue, a living organism, or from an artificial source. RNA molecules within the samples are labeled in a RNA-specific manner prior to immobilization on a solid support. One label is used to mark the location of the RNA molecule on the solid support, whereas the second label is used to mark selectively the S? end of full-length mRNA molecules. RNA molecules are sequenced while being bound to the solid support in one or more sequencing reactions, and sequences of individual RNA molecules can be forwarded to computational analysis for assembling sequence information from individual sequencing reads obtained from the same location on the solid support.Type: ApplicationFiled: August 5, 2008Publication date: February 11, 2010Inventors: Yoshihide Hayashizaki, Piero Carninci, Mutsumi Katayama, Shintaro Katayama, Masayoshi Itoh, Matthias Harbers
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Patent number: 6635215Abstract: Polycarbosilane is mixed with 5-35 wt % of polyvinylsilane to prepare a silicon-base polymer blend which is impregnated in silicon carbide fibers or fabrics to form a preceramic molding body which is exposed to an ionizing radiation to be rendered curing and then fired in an inert gas to produce a composite in which the silicon carbide matrix is reinforced with the silicon carbide fibers.Type: GrantFiled: September 19, 2001Date of Patent: October 21, 2003Assignee: Japan Atomic Energy Research InstituteInventors: Masaki Sugimoto, Yosuke Morita, Kiyohito Okamura, Masayoshi Itoh
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Publication number: 20030065117Abstract: It is here intended to provide a modified silicone compound having excellent heat-resistant properties and a process of producing the same, and there is disclosed a modified silicone compound having an Si—H bond and an unsaturated bond, represented by the general formula (1): 1Type: ApplicationFiled: September 9, 2002Publication date: April 3, 2003Inventors: Narsi Reddy Poreddy, Teruyuki Hayashi, Masato Tanaka, Jun-ichi Ishikawa, Kenji Iwata, Takaharu Abe, Masayoshi Itoh
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Publication number: 20020056946Abstract: Polycarbosilane is mixed with 5-35 wt % of polyvinylsilane to prepare a silicon-base polymer blend which is impregnated in silicon carbide fibers or fabrics to form a preceramic molding body which is exposed to an ionizing radiation to be rendered curing and then fired in an inert gas to produce a composite in which the silicon carbide matrix is reinforced with the silicon carbide fibers.Type: ApplicationFiled: September 19, 2001Publication date: May 16, 2002Inventors: Masaki Sugimoto, Yosuke Morita, Kiyohito Okamura, Masayoshi Itoh
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Patent number: 5620934Abstract: Disclosed herein is a process for the preparation of alkylsilanes and alkenyl silanes by hydrosilylation of their corresponding silanes and unsaturated hydrocarbons in the presence or absence of a catalyst, a process for the preparation of polyalkenylsilanes (some of which are high molecular compounds) by the anionic coordination polymerization, radical polymerization or ionic polymerization of the alkenylsilanes, and a process for the production of silicon carbide by using these polyalkenylsilanes as prepolymers.Type: GrantFiled: March 29, 1995Date of Patent: April 15, 1997Assignee: Mitsui Toatsu Chemicals, IncorporatedInventors: Masayoshi Itoh, Ryo Takeuchi, Kenji Iwata, Mineo Kobayashi
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Patent number: 5596117Abstract: A process for the preparation of alkylsilanes and alkenyl silanes by hydrosilylation of their corresponding silanes and unsaturated hydrocarbons in the presence or absence of a catalyst. A process for the preparation of polyalkenylsilanes (some of which are novel high molecular compounds) by the anionic coordination polymerization, radical polymerization or ionic polymerization of the alkenylsilanes. A process for the production of silicon carbide by using these polyalkenylsilanes as prepolymers.Type: GrantFiled: February 3, 1995Date of Patent: January 21, 1997Assignee: Mitsui Toatsu Chemicals, IncorporatedInventors: Masayoshi Itoh, Ryo Takeuchi, Kenji Iwata
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Patent number: 5508363Abstract: Disclosed herein are a novel process for the preparation of alkylsilanes and alkenyl silanes by hydrosilylation of their corresponding silanes and unsaturated hydrocarbons in the presence or absence of a catalyst, a process for the preparation of polyalkenylsilanes (some of which are novel high molecular compounds) by the anionic coordination polymerization, radical polymerization or ionic polymerization of the alkenylsilanes, and a process for the production of silicon carbide by using these polyalkenylsilanes as prepolymers.Type: GrantFiled: March 29, 1995Date of Patent: April 16, 1996Assignee: Mitsui Toatsu Chemicals, IncorporatedInventors: Masayoshi Itoh, Ryo Takeuchi, Kenji Iwata
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Patent number: 5245542Abstract: A motor vehicle output power control system effects a slip control process for preventing the drive wheels of a motor vehicle from slipping against unstable maneuverability and a trace control process for preventing the motor vehicle from failing to make a turn due to an excessive lateral acceleration. The motor vehicle output power control system has a torque control unit for reducing the drive torque produced by the engine irrespective of controlling action of the driver. A target speed for the drive wheels is established on the basis of the motor vehicle speed, and a reference drive torque for the engine is set depending on the target speed for the drive wheels. Based on a slip of the drive wheels, a target drive torque for the engine is established. The torque control unit is controlled by an electronic controller so that the drive torque produced by the engine is equalized to the target drive torque.Type: GrantFiled: February 27, 1991Date of Patent: September 14, 1993Assignee: Mitsubishi Jidosha Kogyo Kabushiki KaishaInventors: Masayoshi Itoh, Susumu Nishikawa, Yasunobu Miyata
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Patent number: 5197008Abstract: A motor vehicle output power control system quickly reduces the drive torque produced by an engine on a motor vehicle depending on the magnitude of a lateral acceleration which is produced when the motor vehicle makes a turn, or the amount by which drive wheels slips when the motor vehicle is accelerated. A target drive torque depending on the lateral acceleration and a demanded drive torque demanded by the driver of the motor vehicle are added at a predetermined ratio into a turn target drive torque, which is selected as a final target drive torque. The motor vehicle output power control system includes an electronic control unit for controlling an actuator to lower the output drive torque of the engine, independent from controlling action of the driver, such that the output drive torque of the engine is equalized to the final target drive torque.Type: GrantFiled: January 25, 1991Date of Patent: March 23, 1993Assignee: Mitsubishi Jidosha Kokyo Kabushiki KaishaInventors: Masayoshi Itoh, Kiichi Yamada, Hiroaki Yoshida, Katsunori Ohtake, Yasunobu Miyata, Masayuki Hashiguchi, Masanori Tani, Keiji Isoda, Toshio Shigehara, Hiroo Yuasa
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Patent number: 5134903Abstract: A shift control device includes a forward and reverse shifting mechanism for selectively drivingly connecting and disconnecting forward and reverse gear trains to and from a vehicle drive wheel. In shifting the transmission from reverse through neutral and into drive, the forward and reverse shift mechanism remains operatively engaged with the reverse gear train until the transmission is shifted from neutral into drive, at which time the forward and reverse shift mechanism moves into operative engagement with the forward gear train. In shifting from drive through neutral into reverse, the forward and reverse shift mechanism remains operatively engaged with the forward gear train until the transmission is shifted from neutral into reverse, at which time the forward and reverse shift mechanism is moved into operative engagement with the reverse gear train.Type: GrantFiled: June 25, 1991Date of Patent: August 4, 1992Assignee: Suzuki Motor CorporationInventor: Masayoshi Itoh
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Patent number: 5041587Abstract: A process for preparing an organic silicon compound is here disclosed which comprises the step of reacting a halogenated hydrocarbon selected from the group consisting ##STR1## wherein each of X.sup.1 to X.sup.16 is selected from the group consisting of hydrogen, an alkyl group, alkenyl group, phenyl group, naphthyl group, alkoxy group, acyl group, alkylamino group and dialkylamino group having 1 to 20 carbon atoms which are unsubstituted or substituted and a halogen atom; and each of at least one of X.sup.1 to X.sup.4, at least one of X.sup.8 to X.sup.10 and at least one of X.sup.11 to X.sup.16 is a halogen atom,with a silane selected from the group consisting of SiH.sub.4, Si.sub.2 H.sub.6 and Si.sub.3 H.sub.8.Type: GrantFiled: February 12, 1990Date of Patent: August 20, 1991Assignee: Mitsui Toatsu Chemicals, Inc.Inventors: Masayoshi Itoh, Kenji Iwata, Noriyuki Yanagawa, Tetsura Utsumi, Mineo Kobayashi, Ryo Takeuchi, Tomohiro Abe