Patents by Inventor Masayoshi TSUKAHARA
Masayoshi TSUKAHARA has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 10358671Abstract: This invention relates to a method for producing a protein of interest, comprising introducing a protein expression vector which comprises a gene fragment a gene fragment comprising a DNA encoding a protein of interest and a selectable marker gene and transposon sequences at both terminals of the gene fragment, into a suspension mammalian cell; integrating the gene fragment inserted between a pair of the transposon sequences, into a chromosome of the mammalian cell to obtain a mammalian cell capable of expressing the protein of interest; and suspension-culturing the mammalian cell; and a suspension mammalian cell capable of expressing the protein of interest.Type: GrantFiled: June 30, 2017Date of Patent: July 23, 2019Assignees: KYOWA HAKKO KIRIN CO., LTD, Inter-University Research Institute Corporation Research Organization of Information and SystemsInventors: Koichi Kawakami, Keina Yamaguchi, Risa Ogawa, Masayoshi Tsukahara
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Publication number: 20190194621Abstract: An object of the present invention is to provide a technique for stably, inexpensively, and safely culturing stem cells having a differentiation ability while maintaining an undifferentiated state. The present invention relates to a medium for culturing stem cells, comprising at least one or more compounds selected from the group consisting of a ROCK inhibitor, a PKC inhibitor, a histone methyltransferase inhibitor, and a retinoic acid receptor agonist, and not containing a growth factor or having a low growth factor concentration; a method for culturing stem cells using the medium; a growth promoter for stem cell; as well as a cell composition containing stem cells or differentiated cells therefrom; and a method for producing the cell composition.Type: ApplicationFiled: September 8, 2017Publication date: June 27, 2019Applicants: KYOWA HAKKO BIO CO., LTD., KYOWA HAKKO KIRIN CO., LTD.Inventors: Kana YAMAGUCHI, Shinichiro SHOJI, Masayoshi TSUKAHARA
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Publication number: 20190177686Abstract: An object of the present invention is to provide an albumin-free medium for serum-free culture of pluripotent stem cells capable of maintaining the undifferentiated state and the pluripotency (pluripotent ability) of the pluripotent stem cells, an additive for an albumin-free medium, and a culture method. The albumin-free medium for serum-free culture of pluripotent stem cells, the additive for an albumin-free medium, and a medium used for the culture method of the invention are characterized by containing at least one selected from the group consisting of a Pluronic nonionic surfactant, an animal-based hydrolysate, and a nonanimal-based hydrolysate.Type: ApplicationFiled: May 8, 2017Publication date: June 13, 2019Applicant: KYOWA HAKKO BIO CO., LTD.Inventors: Miho FURUE, Kana YANAGIHARA, Shinichiro SHOJI, Masayoshi TSUKAHARA
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Publication number: 20190127707Abstract: An object of the present invention is to provide a medium for culturing naive pluripotent stem cells which is for efficiently proliferating naive pluripotent stem cells while maintaining the undifferentiated state and the pluripotency (pluripotent capability) of the naive pluripotent stem cells.Type: ApplicationFiled: March 30, 2017Publication date: May 2, 2019Applicant: KYOWA HAKKO BIO CO., LTD.Inventors: Miho FURUE, Shinichiro SHOJI, Kana YANAGIHARA, Masayoshi TSUKAHARA
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Publication number: 20170306378Abstract: This invention relates to a method for producing a protein of interest, comprising introducing a protein expression vector which comprises a gene fragment a gene fragment comprising a DNA encoding a protein of interest and a selectable marker gene and transposon sequences at both terminals of the gene fragment, into a suspension mammalian cell; integrating the gene fragment inserted between a pair of the transposon sequences, into a chromosome of the mammalian cell to obtain a mammalian cell capable of expressing the protein of interest; and suspension-culturing the mammalian cell; and a suspension mammalian cell capable of expressing the protein of interest.Type: ApplicationFiled: June 30, 2017Publication date: October 26, 2017Applicants: KYOWA HAKKO KIRIN CO., LTD, Inter-University Research Institute Corporation Re search Organization of Information and SystemsInventors: Koichi KAWAKAMI, Keina YAMAGUCHI, Risa OGAWA, Masayoshi TSUKAHARA
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Patent number: 9725750Abstract: This invention relates to a method for producing a protein of interest, comprising introducing a protein expression vector which comprises a gene fragment a gene fragment comprising a DNA encoding a protein of interest and a selectable marker gene and transposon sequences at both terminals of the gene fragment, into a suspension mammalian cell; integrating the gene fragment inserted between a pair of the transposon sequences, into a chromosome of the mammalian cell to obtain a mammalian cell capable of expressing the protein of interest; and suspension-culturing the mammalian cell; and a suspension mammalian cell capable of expressing the protein of interest.Type: GrantFiled: April 17, 2015Date of Patent: August 8, 2017Assignees: KYOWA HAKKO KIRIN CO., LTD, Inter-University Research Institute Corporation Research Organization of Information and SystemsInventors: Koichi Kawakami, Keina Yamaguchi, Risa Ogawa, Masayoshi Tsukahara
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Publication number: 20170198262Abstract: As a technique capable of culturing anchorage-dependent cells without using an anchorage, provided is a medium for anchorage-dependent cells, which comprises MFG-E8 (Milk fat globule-EGF factor 8) or a fragment of the protein. This medium can promote adhesion of anchorage-dependent cells in the absence of an anchorage, enables the survival and proliferation (colony formation) of the cells, and further, also enables the subsequent differentiation if the anchorage-dependent cells are stem cells.Type: ApplicationFiled: May 28, 2015Publication date: July 13, 2017Applicant: Kyowa Hakko Kirin Co., Ltd.Inventors: Yoshiki NAKASHIMA, Takeshi OMASA, Masayoshi TSUKAHARA
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Patent number: 9410176Abstract: This invention relates to a method for producing a protein of interest, comprising introducing an expression vector which comprises a gene fragment comprising a DNA encoding the protein of interest and a selectable marker gene and also comprises a pair of transposon sequences at both terminals of the gene fragment, into a suspension mammalian cell; integrating the gene fragment inserted between the pair of transposon sequences into a chromosome of the mammalian cell; obtaining a suspension mammalian cell producing the protein of interest; and suspension-culturing the suspension mammalian cell, and a suspension mammalian cell which expresses the protein of interest by the method.Type: GrantFiled: December 14, 2011Date of Patent: August 9, 2016Assignees: Inter-University Research Institute Corporation Research Organization of Information and Systems, KYOWA HAKKO KIRIN CO., LTD.Inventors: Megumi Kurokawa, Yoko Hayashi, Masayoshi Tsukahara
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Patent number: 9102971Abstract: The present invention relates to a method for integrating a gene fragment inserted between a pair of transposon sequences into a chromosome of a mammalian cell, comprising introducing at least one expression vector which comprises a gene fragment comprising a DNA encoding a protein of interest and also comprises a pair of transposon sequences at both terminals of the gene fragment, into a suspension mammalian cell; and a method for producing the protein of interest comprising suspension-culturing a suspension mammalian cell which produces the protein of interest; and an a suspension mammalian cell which expresses the protein of interest.Type: GrantFiled: December 15, 2011Date of Patent: August 11, 2015Assignees: Inter-University Research Institute Corporation Research Organization of Information and Systems, KYOWA HAKKO KIRIN CO., LTDInventors: Megumi Kurokawa, Keina Yamaguchi, Risa Ogawa, Masayoshi Tsukahara, Koichi Kawakami, Yoko Hayashi
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Publication number: 20150218610Abstract: This invention relates to a method for producing a protein of interest, comprising introducing a protein expression vector which comprises a gene fragment a gene fragment comprising a DNA encoding a protein of interest and a selectable marker gene and transposon sequences at both terminals of the gene fragment, into a suspension mammalian cell; integrating the gene fragment inserted between a pair of the transposon sequences, into a chromosome of the mammalian cell to obtain a mammalian cell capable of expressing the protein of interest; and suspension-culturing the mammalian cell; and a suspension mammalian cell capable of expressing the protein of interest.Type: ApplicationFiled: April 17, 2015Publication date: August 6, 2015Applicants: KYOWA HAKKO KIRIN CO., LTD, Inter-University Research-Institute Corporation Research Organization of Information & SystemsInventors: Koichi KAWAKAMI, Keina YAMAGUCHI, Risa OGAWA, Masayoshi TSUKAHARA
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Patent number: 9034649Abstract: This invention relates to a method for producing a protein of interest, comprising introducing a protein expression vector which comprises a gene fragment a gene fragment comprising a DNA encoding a protein of interest and a selectable marker gene and transposon sequences at both terminals of the gene fragment, into a suspension mammalian cell; integrating the gene fragment inserted between a pair of the transposon sequences, into a chromosome of the mammalian cell to obtain a mammalian cell capable of expressing the protein of interest; and suspension-culturing the mammalian cell; and a suspension mammalian cell capable of expressing the protein of interest.Type: GrantFiled: June 11, 2010Date of Patent: May 19, 2015Assignees: Inter-University Research Institute Corporation Research Organization of Information and Systems, KYOWA HAKKO KIRIN CO., LTDInventors: Koichi Kawakami, Keina Yamaguchi, Risa Ogawa, Masayoshi Tsukahara
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Publication number: 20130273604Abstract: This invention relates to a method for producing a protein of interest, comprising introducing an expression vector which comprises a gene fragment comprising a DNA encoding the protein of interest and a selectable marker gene and also comprises a pair of transposon sequences at both terminals of the gene fragment, into a suspension mammalian cell; integrating the gene fragment inserted between the pair of transposon sequences into a chromosome of the mammalian cell; obtaining a suspension mammalian cell producing the protein of interest; and suspension-culturing the suspension mammalian cell, and a suspension mammalian cell which expresses the protein of interest by the method.Type: ApplicationFiled: December 14, 2011Publication date: October 17, 2013Applicant: KYOWA HAKKO KIRIN CO., LTD.Inventors: Megumi Kurokawa, Yoko Hayashi, Masayoshi Tsukahara
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Publication number: 20120196327Abstract: The present invention relates to a method for integrating a gene fragment inserted between a pair of transposon sequences into a chromosome of a mammalian cell, comprising introducing at least one expression vector which comprises a gene fragment comprising a DNA encoding a protein of interest and also comprises a pair of transposon sequences at both terminals of the gene fragment, into a suspension mammalian cell; and a method for producing the protein of interest comprising suspension-culturing a suspension mammalian cell which produces the protein of interest; and an a suspension mammalian cell which expresses the protein of interest.Type: ApplicationFiled: December 15, 2011Publication date: August 2, 2012Applicants: Kyowa Hakko Kirin Co., Ltd., Inter-University Research Institute Corporation Research Organization of Information and SystemsInventors: Megumi KUROKAWA, Keina YAMAGUCHI, Risa OGAWA, Masayoshi TSUKAHARA, Koichi KAWAKAMI, Yoko HAYASHI
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Publication number: 20110045532Abstract: This invention relates to a method for producing a protein of interest, comprising introducing a protein expression vector which comprises a gene fragment a gene fragment comprising a DNA encoding a protein of interest and a selectable marker gene and transposon sequences at both terminals of the gene fragment, into a suspension mammalian cell; integrating the gene fragment inserted between a pair of the transposon sequences, into a chromosome of the mammalian cell to obtain a mammalian cell capable of expressing the protein of interest; and suspension-culturing the mammalian cell; and a suspension mammalian cell capable of expressing the protein of interest.Type: ApplicationFiled: June 11, 2010Publication date: February 24, 2011Applicants: Inter-University Research Institute Corporation, Research Organization of Information and systems, KYOWA HAKKO KIRIN CO., LTD.Inventors: Koichi KAWAKAMI, Keina YAMAGUCHI, Risa OGAWA, Masayoshi TSUKAHARA