Abstract: A method for determining total chlorine amount present in a sample by mixing the sample with a benzidine indicator solution comprising a benzidine compound capable of forming a dye by a reaction with a chlorine, wherein the hue of the formed dye changes depending upon the mole ratio of the chlorine to benzidine compound and determining the total chlorine amount from the hue. A kit for practicing the method incudes the indicator and a color scale for the hues of the dye.

Abstract: A method for detecting protein present on the surface of a sample and a kit for performing protein detection applying the detecting method are disclosed. The method is comprising the steps of (1) transferring substances present on the subject portion of the surface of a sample to a sampling means which comprises a water absorbable portion comprising a water-insluble sythetic polymer, (2) contacting the substances transferred to said sampling means with a reagent capable of forming color upon reaction with protein, and (3) measuring color formed by the reaction of said reagent with protein to determine the amount of protein contained in the substance transferred to the sapling means. The kit includes the sampling means and the color forming reagent in combination.