Abstract: A method of detecting a nucleic acid including mixing a fluid including a target nucleic acid with a detection reagent including a first enzyme for cleaving a first nucleic acid having a first flap and a second enzyme for cleaving a second nucleic acid such that the target nucleic acid, the first nucleic acid and the second nucleic acid form a complex as a first invasive structure, conducting a first reaction which causes the first enzyme to cleave the first flap of the first invasive structure and produces a third nucleic acid that forms a complex, as a second invasive structure, with a fourth nucleic acid having a second flap, and conducting a second reaction which causes the second enzyme to cleave the second flap of the second invasive structure and produces a cleaved product.

Abstract: A reaction container includes a base material, an analysis well in which a nucleic acid analysis reagent used for a nucleic acid analysis is positioned, the analysis well being arranged on the base material and configured to analyze a sample containing a nucleic acid, and a quantification well in which a quantification reagent configured to specifically detect the nucleic acid is positioned, the quantification well being arranged on the base material and configured to quantify an amount of the nucleic acid contained in the sample, in which the nucleic acid contained in the sample is distributed into the quantification well and the analysis well.

Abstract: A reaction container includes a base material, an analysis well in which a nucleic acid analysis reagent used for a nucleic acid analysis is positioned, the analysis well being arranged on the base material and configured to analyze a sample containing a nucleic acid, and a quantification well in which a quantification reagent configured to specifically detect the nucleic acid is positioned, the quantification well being arranged on the base material and configured to quantify an amount of the nucleic acid contained in the sample, in which the nucleic acid contained in the sample is distributed into the quantification well and the analysis well.

Abstract: The present invention provides a manufacturing method that can easily manufacture a compound known as photoresponsive (photocoupling) nucleic acids at high yield in a shorter period of time than that of the conventional technology. The present invention relates to a method of manufacturing a photoresponsive nucleic acid which includes a step of reacting a nucleic acid having groups represented by the Formula I, the Formula III, the Formula IV, or the Formula V and a compound represented by the Formula II, or reacting a nucleic acid having groups represented by the Formula VI, the Formula VIII, the Formula IX, or the Formula X and a compound represented by the Formula VII by heating them by microwaves in the presence of a metal catalyst, a basic substance, and a solvent.

Abstract: The present invention provides a manufacturing method that can easily manufacture a compound known as photoresponsive (photocoupling) nucleic acids at high yield in a shorter period of time than that of the conventional technology. The present invention relates to a method of manufacturing a photoresponsive nucleic acid which includes a step of reacting a nucleic acid having groups represented by the Formula I, the Formula III, the Formula IV, or the Formula V and a compound represented by the Formula II, or reacting a nucleic acid having groups represented by the Formula VI, the Formula VIII, the Formula IX, or the Formula X and a compound represented by the Formula VII by heating them by microwaves in the presence of a metal catalyst, a basic substance, and a solvent.

Abstract: The present invention provides a method for detecting methylcytosine in DNA rapidly, conveniently, and with high sensitivity. The present invention relates to a method for detecting methylcytosine by using a methylcytosine photocoupling agent (a photoresponsive probe) consisting of nucleic acids having a group represented by the Formula (I), (II), (III) or (IV) as a base moiety.

Abstract: The present invention provides a manufacturing method that can easily manufacture a compound known as photoresponsive (photocoupling) nucleic acids at high yield in a shorter period of time than that of the conventional technology. The present invention relates to a method of manufacturing a photoresponsive nucleic acid which includes a step of reacting a nucleic acid having groups represented by the Formula I, the Formula III, the Formula IV, or the Formula V and a compound represented by the Formula II, or reacting a nucleic acid having groups represented by the Formula VI, the Formula VIII, the Formula IX, or the Formula X and a compound represented by the Formula VII by heating them by microwaves in the presence of a metal catalyst, a basic substance, and a solvent.

Abstract: The present invention provides a method for detecting methylcytosine in DNA rapidly, conveniently, and with high sensitivity. The present invention relates to a method for detecting methylcytosine by using a methylcytosine photocoupling agent (a photoresponsive probe) consisting of nucleic acids having a group represented by the Formula (I), (II), (III) or (IV) as a base moiety.

Abstract: The present invention provides a manufacturing method that can easily manufacture a compound known as photoresponsive (photocoupling) nucleic acids at high yield in a shorter period of time than that of the conventional technology. The present invention relates to a method of manufacturing a photoresponsive nucleic acid which includes a step of reacting a nucleic acid having groups represented by the Formula I, the Formula III, the Formula IV, or the Formula V and a compound represented by the Formula II, or reacting a nucleic acid having groups represented by the Formula VI, the Formula VIII, the Formula IX, or the Formula X and a compound represented by the Formula VII by heating them by microwaves in the presence of a metal catalyst, a basic substance, and a solvent.

Abstract: A layer is provided for bonding a conductive plating to a substrate which substrate normally does not provide firm bonding of plating directly thereto. The bonding layer forms a matrix in which are dispersed ultrafine metal particles. The bonding layer is formed in situ on the substrate surface with the metal particles being maintained unaggregated in the matrix as it is formed on the surface. Plating subsequently formed on the outer surface of the bonding layer is effectively firmly bonded to the substrate through the intermediary of the matrix and metal particles distributed therein. The bonding layer includes a fixative formed of an organic compound including a metal and an organic solvent. The bonding layer may further include a film strengthener.

Abstract: A colorant of ultrafine particles for providing transparent color includes ultrafine metal particles, a fixing agent and a glass forming material. The ultrafine metal particles can be gas condensed ultrafine metal particles or polymer compound ultrafine metal particles. The colorant produces a transparent colored film on a substrate.

Abstract: A screen forming apparatus and method comprises a long pipe of predetermined length floatable in the water, a pump for conveying the water through the pipe, a pipe connecting the pipe with the pump, an air-water device provided with the pipe for mixing the water with air, a number of nozzles disposed along the longitudinal direction of the pipe at predetermined intervals for injecting the water in the form of screen, and a device for producing air curtains flowing along the front and the rear surfaces of the screen in order to maintain the screen substantially uniform in thickness.

Abstract: A screen forming apparatus and method comprises a long pipe of predetermined length floatable in the water, a pump for conveying the water through the pipe, a pipe connecting the pipe with the pump, an air-water device provided with the pipe for mixing the water with air, a number of nozzles disposed along the longitudinal direction of the pipe at predetermined intervals for injecting the water in the form of screen, and a device for producing air curtains flowing along the front and the rear surfaces of the screen in order to maintain the screen substantially uniform in thickness.