Abstract: A combined electric power generations' supply system includes an AC power generator that supplies power by off grid independent operation, a DC power supply device, an inverter that converts DC power output by the DC power supply device into AC power. The rotation calculation unit calculates a value relating to a rotation of a rotor when driving the virtual power generator according to an active power command based on a rotor model calculates a value relating to the rotation of the rotor of the virtual power generator and the active power command. The output determination unit determines values relating to an active power and a reactive power to be output to the inverter based on the calculated value relating to the rotation. The modulation control unit performs control of a pulse width modulation of the inverter based on the determined value relating to the active power and reactive power.

Abstract: A nucleic amplifier is provided to reduce an amplification efficiency drop even with a short cycle time. In the nucleic amplifier, the denaturation phase of moving and retaining a liquid droplet in a first region of a container heated to a denaturation temperature of a target nucleic acid, and the synthesis phase of moving and retaining the liquid droplet in a second region of the container different from the first region are repeated in multiple cycles. The liquid droplet contains a fluorescently labeled probe. The fluorescently labeled probe contains a minor groove binder molecule.

Abstract: A nucleic acid amplification reaction method includes a thermal cycling step of performing thermal cycling for amplifying a nucleic acid for a reaction solution containing a primer, a temperature increasing step of increasing the temperature of the reaction solution to a temperature at which the amplified nucleic acid is denatured after the thermal cycling step, a temperature decreasing step of decreasing the temperature of the reaction solution to a temperature at which a probe hybridizes after the temperature increasing step, and a probe addition step of adding the probe to the reaction solution.

Abstract: A nucleic acid amplification reaction method includes performing thermal cycling for amplifying a nucleic acid for a reaction solution containing a primer and a probe, wherein in the thermal cycling, the time per cycle of the thermal cycling is 9 seconds or less, and the Tm value of the primer is 70° C. or higher and 80° C. or lower.

Abstract: A nucleic acid amplification reaction method includes a heating step of heating a reaction solution containing a reverse transcriptase, a polymerase, a primer, and a probe for performing a reverse transcription reaction, and a thermal cycling step of performing thermal cycling for amplifying a nucleic acid for the reaction solution after the heating step, wherein the Tm value of the primer is 65° C. or higher and 80° C. or lower, and in the heating step, a heating time for the reaction solution is 5 seconds or more and 480 seconds or less.

Abstract: A nucleic acid amplification reaction method includes performing thermal cycling for amplifying a nucleic acid for a reaction solution containing a template nucleic acid, a primer, a probe, and a polymerase, wherein in the thermal cycling, the time per cycle of the thermal cycling is 9 seconds or less, the calculated Tm value of the primer is 65° C. or higher and 80° C. or lower, a ?Tm value obtained by subtracting the actually measured Tm value of the primer from the actually measured Tm value of the probe is ?11° C. or more and 2° C. or less, the calculated Tm value is a value calculated according to a calculation formula, and the actually measured Tm value is an actually measured value obtained by actual measurement.

Abstract: A nucleic acid amplification method includes a step of heating a first region of a container housing a droplet containing a target nucleic acid and a sample necessary for amplification of the target nucleic acid to a denaturation temperature of the target nucleic acid and heating a second region different from the first region to a synthesis temperature of the target nucleic acid, and an amplification step of repeating a cycle through a denaturation stage at which the droplet housed in the container is moved to and retained in the first region and a synthesis stage at which the droplet is moved to and retained in the second region at a plurality of times. At the amplification step, periods of part of cycles of the plurality of cycles are made shorter than periods of the other cycles.

Abstract: A nucleic acid amplification reaction method includes subjecting a reaction mixture containing a nucleic acid amplification reaction reagent to be used for amplifying a nucleic acid to a thermal cycle for amplifying the nucleic acid, wherein in the thermal cycle, a heating time for an annealing reaction and an elongation reaction is 1 sec or more and 10 sec or less, the nucleic acid amplification reaction reagent contains a forward primer, a reverse primer, a polymerase, and a fluorescently labeled probe, the concentration of the forward primer is 0.4 ?M or more and 3.2 ?M or less, the concentration of the reverse primer is 0.4 ?M or more and 3.2 ?M or less, the amount of the polymerase is 0.5 U or more and 4 U or less, and the concentration of the fluorescently labeled probe is 0.15 ?M or more and 1.2 ?M or less.

Abstract: A nucleic acid amplification reagent includes a probe which anneals to a target nucleic acid contained in a nucleic acid, and an intercalator which is inserted between base pairs of one strand of the nucleic acid and a complementary strand synthesized on the one strand, and between base pairs of the other strand of the nucleic acid and a complementary strand synthesized on the other strand. The wavelength band of light emitted from the probe and the wavelength band of light emitted from the intercalator at least partially overlap with each other.

Abstract: A nucleic acid amplification reagent includes a first probe which anneals to a target nucleic acid in a region sandwiched between a site to which the 3? end of a forward primer anneals and a site to which the 3? end of a reverse primer anneals in a template nucleic acid, and a second probe which anneals to a nucleic acid other than the target nucleic acid in the region. Each of the first probe and the second probe includes a dye which emits light in a wavelength band mutually overlapping partially.

Abstract: A nucleic acid amplification reaction apparatus includes: a fitting section capable of fitting a reaction vessel filled with a reaction mixture and a liquid which has a specific gravity different from that of the reaction mixture and is immiscible with the reaction mixture; a first heating section which heats a first region of the reaction vessel; a second heating section which heats a second region of the reaction vessel; and a driving mechanism which switches over between a first arrangement in which the first region is located lower than the second region in the direction of the gravitational force and a second arrangement in which the second region is located lower than the first region in the direction of the gravitational force, wherein the concentration of magnesium ions in the reaction mixture is 1.8 mM or more and 9.0 mM or less.

Abstract: A nucleic acid amplification method includes: heating a first region of a nucleic acid amplification reaction vessel filled with a nucleic acid amplification reaction mixture and a liquid which has a specific gravity different from that of the nucleic acid amplification reaction mixture and is immiscible with the nucleic acid amplification reaction mixture to a first temperature; heating a second region of the nucleic acid amplification reaction vessel to a second temperature; switching over from a first arrangement in which the first region is located lower than the second region in the direction of the gravitational force to a second arrangement in which the second region is located lower than the first region in the direction of the gravitational force; and switching over from the second arrangement to the first arrangement, wherein the nucleic acid amplification reaction mixture contains a probe, and the probe contains an artificial nucleic acid.

Abstract: A method for discriminating the presence or absence of a mutation in a DNA having a plurality of mutated forms includes performing a thermal cycle for amplifying the DNA in the presence of a pair of primers which can bind to the DNA, a first probe which does not bind to the DNA having a mutation, can bind to the DNA having no mutation, and is labeled with a first fluorescent substance, and a second probe which can bind to both of the DNA having a mutation and the DNA having no mutation and is labeled with a second fluorescent substance which emits a light having a fluorescence wavelength different from that of a light emitted from the first fluorescent substance.

Abstract: A nucleic acid amplification method includes: forming a first liquid droplet containing a first reaction reagent for amplifying a first nucleic acid in one vessel containing a silicone oil to which a fluoro-modified silicone resin is added; forming a second liquid droplet containing a second reaction reagent for amplifying a second nucleic acid in the one vessel; and performing a nucleic acid amplification reaction in the vessel by fitting the one vessel in a nucleic acid amplification reaction apparatus while maintaining the first liquid droplet independent of the second liquid droplet and then operating the nucleic acid amplification reaction apparatus.

Abstract: A TFT of the present invention includes a gate electrode, a gate insulating film and a first semiconductor film which are sequentially formed on an insulating substrate, a second semiconductor film including a high density impurity which is formed on the first semiconductor film while being separated into portions at grade and a first electrode and a second electrode, each of which is formed on the separated second semiconductor film. Further, a peripheral portion of the first semiconductor film includes a protruded portion toward the outside from an edge of the second semiconductor film, and a surface of the protruded portion is roughened. By roughening the surface of the protruded portion, an on-current of the TFT can be maintained and the leakage current can be suppressed.

Abstract: An apparatus for combustion harmfulness-elimination of PFC gas is provided, which prevents or effectively suppress the generation of byproducts and the corrosion of the inner walls of the apparatus. This apparatus comprises (a) a combustion furnace; (b) a gas introduction section formed near the combustion furnace; (c) a wet scrubber for wet-scrubbing a first gas; and (d) a connection pipe for connecting the gas introduction section to the scrubber; the connection pipe having a branch extending downward. Even if moisture is absorbed into the first gas during the wet-scrubbing process in the wet scrubber, the moisture in the first gas is automatically separated therefrom in a liquid state and flows out of the apparatus through the branch prior to reaching the gas introduction section.

Abstract: A TFT of the present invention includes a gate electrode, a gate insulating film and a first semiconductor film which are sequentially formed on an insulating substrate, a second semiconductor film including a high density impurity which is formed on the first semiconductor film while being separated into portions at grade and a first electrode and a second electrode, each of which is formed on the separated second semiconductor film. Further, a peripheral portion of the first semiconductor film includes a protruded portion toward the outside from an edge of the second semiconductor film, and a surface of the protruded portion is roughened. By roughening the surface of the protruded portion, an on-current of the TFT can be maintained and the leakage current can be suppressed.

Abstract: An apparatus for combustion toxicity-elimination of PFC gas is provided, which prevents or effectively suppress the generation of byproducts and the corrosion of the inner walls of the apparatus. This apparatus comprises (a) a combustion furnace; (b) a gas introduction section formed near the combustion furnace; (c) a wet scrubber for wet-scrubbing a first gas; and (d) a connection pipe for connecting the gas introduction section to the scrubber; the connection pipe having a branch extending downward. Even if moisture is absorbed into the first gas during the wet-scrubbing process in the wet scrubber, the moisture in the first gas is automatically separated therefrom in a liquid state and flows out of the apparatus through the branch prior to reaching the gas introduction section.

Abstract: A drag mechanism for a fishing reel having a spool shaft supported at both the ends thereof. The drag mechanism employs rolling members as drag members so that the durability of the mechanism is enhanced, and the dragging function thereof is kept stable and good for a long time even under severe conditions under which sea water, sand or the like is likely to cling to the mechanism. One of drag member holders is secured to the spool of the reel so as to be rotated together with the spool. The other of the holders is secured to the spool shaft so as to be rotated together therewith. The tapered outside circumferential surface of one of the holders faces the tapered inside circumferential surface of the other of them. The rolling members are fitted in between the tapered surfaces. The drag mechanism can be used to drag the reverse rotation of the spool by frictional resistances to the rolling and sliding of the rolling members.

Abstract: A spool (3) is rotatably fitted to a support shaft (2) of a reel main body (1). The spool (3) is formed of aluminum in a cylindrical shape by die casting and includes a winding drum portion (3a), around which a fishing line (not show) is to be wound, and two flanges (3b and 3c) respectively provided on either side thereof. On the outside of the flange portion (3b) of the spool (3) disposed on the opening side of the reel main body (1), there is formed a notch portion (3d) to the outside of which a separate ring (4) is mounted and fixed by adhesives. The ring (4) is formed in a porous pan shape by machining an aluminum member. And, the ring (4) includes an inner peripheral surface (4a) which is to be placed on the outer periphery of the flange (3b) of the spool (3), and an outer peripheral surface (4b) which is formed so as to be substantially level with the outer periphery (1a) of the reel main body (1).