Abstract: Provided is a nucleic acid sample-contained container including; a first nucleic acid molecule including an intended base sequence and a base sequence for detection different from the intended base sequence; and a second nucleic acid molecule free of the intended base sequence but including the base sequence for detection, wherein the nucleic acid sample-contained container includes the first nucleic acid molecule in a predetermined number. In a preferable mode, the copy number of the intended base sequence is less than 1,000, and the coefficient of variation (CV value) for the copy number is lower than 20%. In a more preferable mode, the nucleic acid molecules are artificially synthesized nucleic acid molecules. In a yet more preferable mode, the first nucleic acid molecule includes the intended base sequence in a plural number in the same molecule.

Abstract: There is provided a production method for microglia, including a step of introducing, into pluripotent stem cells, a nucleic acid including an open reading frame of a transcription factor that increases expression of a Complement C3 (C3) gene. In the production method, the transcription factor is selected from the group consisting of SPI1, CEBPA, PTAFR, FLI1, MEF2C, EGR2, RUNX1, TNF, CEBPB, IKZF1, KLF6, NFIC, ELF4, PAX8, PRDM1, MEF2A, and NR4A3.

Abstract: In one embodiment, provided are a method for analyzing at least one nucleic acid that can conveniently and highly accurately analyze even a very small number of analyte at least one nucleic acid. In one embodiment, the present invention relates to a method for analyzing at least one nucleic acid, comprising: a library preparation step of preparing a library comprising at least one standard nucleic acid of specific copy number(s) and at least one analyte nucleic acid in a same system; a calibration curve data generation step of generating calibration curve data based on the copy number(s) of the at least one standard nucleic acid of specific copy number(s); and an analyte nucleic acid analysis step of identifying at least one nucleotide sequence of the analyte nucleic acid while identifying the number(s) of the at least one nucleotide sequence of the at least one analyte nucleic acid using the calibration curve data.

Abstract: An object of the present invention is to provide a technique for precisely arranging nerve cells on a substrate while suppressing the migration of nerve cells. A manufacturing method for a substrate on which nerve cells are arranged is provided, the method including a step of arranging, on a substrate, a plurality of liquid droplets containing nerve cells by an inkjet method to form one or a plurality of liquid pools, the substrate having a region in which a cell adhesive material is arranged and a region in which a cell non-adhesive material is arranged; and a step of incubating the liquid pool until the nerve cells sediment and temporarily adhere onto the substrate to form a cell aggregate. The diameter per one liquid pool is 500 ?m or less, and the density of nerve cells per one liquid pool is 105 cells/cm2 or more.

Abstract: In a nucleic acid detection cassette, a predetermined syringe is crushed to supply a sample solution to an amplification unit via a channel and the amplification unit is heated from outside to amplify sample DNA in the sample solution. The sample solution containing an amplification product is supplied to a detection unit via the channel and a hybridization reaction occurs in the detection unit. Next, another syringe is crushed and an intercalating agent solution is supplied via another predetermined channel. Therefore, a nucleic acid detection cassette capable of automation from amplification of a sample to detection of an electrochemical reaction of the sample is provided.

Abstract: A nucleic acid inspection apparatus has an installer to install a nucleic acid inspection device, the installer comprising a storage unit to store at least a specimen sample, an amplifier to amplify nucleic acid contained in the specimen sample stored in the storage unit, a first flow passage to move the specimen sample from the storage unit to the amplifier, an inspection unit, and a second flow passage to move the specimen sample from the amplifier to the inspection unit, a first opening/closing unit to open/close the first flow passage, a second opening/closing unit to open/close the second flow passage, a heater to heat the amplifier, and a controller to control the first and second opening/closing units so as to open/close in a predetermined order and to control the heater so as to heat the amplifier in conjunction with opening/closing operations of the first and second opening/closing units.

Abstract: In one embodiment, provided are a method for analyzing at least one nucleic acid that can conveniently and highly accurately analyze even a very small number of analyte at least one nucleic acid. In one embodiment, the present invention relates to a method for analyzing at least one nucleic acid, comprising: a library preparation step of preparing a library comprising at least one standard nucleic acid of specific copy number(s) and at least one analyte nucleic acid in a same system; a calibration curve data generation step of generating calibration curve data based on the copy number(s) of the at least one standard nucleic acid of specific copy number(s); and an analyte nucleic acid analysis step of identifying at least one nucleotide sequence of the analyte nucleic acid while identifying the number(s) of the at least one nucleotide sequence of the at least one analyte nucleic acid using the calibration curve data.

Abstract: Provided is a nucleic acid sample-contained container including; a first nucleic acid molecule including an intended base sequence and a base sequence for detection different from the intended base sequence; and a second nucleic acid molecule free of the intended base sequence but including the base sequence for detection, wherein the nucleic acid sample-contained container includes the first nucleic acid molecule in a predetermined number. In a preferable mode, the copy number of the intended base sequence is less than 1,000, and the coefficient of variation (CV value) for the copy number is lower than 20%. In a more preferable mode, the nucleic acid molecules are artificially synthesized nucleic acid molecules. In a yet more preferable mode, the first nucleic acid molecule includes the intended base sequence in a plural number in the same molecule.

Abstract: In a nucleic acid detection cassette, a predetermined syringe is crushed to supply a sample solution to an amplification unit via a channel and the amplification unit is heated from outside to amplify sample DNA in the sample solution. The sample solution containing an amplification product is supplied to a detection unit via the channel and a hybridization reaction occurs in the detection unit. Next, another syringe is crushed and an intercalating agent solution is supplied via another predetermined channel. Therefore, a nucleic acid detection cassette capable of automation from amplification of a sample to detection of an electrochemical reaction of the sample is provided.

Abstract: A nucleic acid inspection apparatus has an installer to install a nucleic acid inspection device, the installer comprising a storage unit to store at least a specimen sample, an amplifier to amplify nucleic acid contained in the specimen sample stored in the storage unit, a first flow passage to move the specimen sample from the storage unit to the amplifier, an inspection unit, and a second flow passage to move the specimen sample from the amplifier to the inspection unit, a first opening/closing unit to open/close the first flow passage, a second opening/closing unit to open/close the second flow passage, a heater to heat the amplifier, and a controller to control the first and second opening/closing units so as to open/close in a predetermined order and to control the heater so as to heat the amplifier in conjunction with opening/closing operations of the first and second opening/closing units.

Abstract: According to one embodiment, a liquid feed device includes a support substrate and an intermediate member. The intermediate member is provided on the support substrate. A valve and a flow channel of fluid are formed by the intermediate member. The valve communicates with the flow channel. The valve includes an outer edge portion and a gap. The gap is provided between the outer edge portion and the support substrate. The valve is capable of opening and closing the gap by the outer edge portion being pressed and released. A configuration of the outer edge portion when projected onto a plane perpendicular to a direction of the flow channel is a protruding configuration having a curved surface. The configuration of the outer edge portion is symmetric with respect to the direction of the flow channel.