Patents by Inventor Masoud M. Toloue

Masoud M. Toloue has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Oligonucleotide ligation methods for improving data quality and throughput using massively parallel sequencing
    Patent number: 9255291
    Abstract: Described herein is a buffer concentration for highly efficient ligation of two oligonucleotides. The embodiments herein have led to the development of an optimized ligation step used in the sample preparation for sequencing reactions. Further, embodiments herein describe a high-throughput method for sequencing using barcodes or the purpose of multiplexing several samples simultaneously and novel methods for making targeted DNA libraries for re-sequencing on massively parallel next-generation sequencing platforms and for alternatives to gel-purification for recovering the desired templates from small RNA libraries for next generation sequencing.
    Type: Grant
    Filed: May 6, 2011
    Date of Patent: February 9, 2016
    Assignee: BIOO SCIENTIFIC CORPORATION
    Inventors: Masoud M. Toloue, Marianna Goldrick
  • TOUCHSCREEN DEVICE AND METHODS FOR USE IN DETECTION OF MICRORNA
    Publication number: 20140342442
    Abstract: A method and sensor for detecting the presence of specific target nucleic acids uses a biosensor comprising an array of single stranded probe oligonucleotides, wherein one end of each probe oligonucleotide is linked to a label and the other end of each probe oligonucleotide is attached to an electrode of a solid support. The method includes determining the capacitance at one or more of the electrodes, where a change in the capacitance of the one or more electrodes indicates the presence of target nucleic acid complementary to the single stranded oligonucleotide probe.
    Type: Application
    Filed: May 15, 2014
    Publication date: November 20, 2014
    Applicant: BIOO SCIENTIFIC CORPORATION
    Inventors: Masoud M. Toloue, Michael T. Mueller, Swapna Gone
  • METHODS FOR IMPROVING LIGATION STEPS TO MINIMIZE BIAS DURING PRODUCTION OF LIBRARIES FOR MASSIVELY PARALLEL SEQUENCING
    Publication number: 20140128292
    Abstract: Described herein is a thermostable enzyme capable of efficient ligation of two oligomers at high temperature. The embodiments herein have led to the development of an optimized ligation step used in library preparation for sequencing reactions.
    Type: Application
    Filed: September 27, 2013
    Publication date: May 8, 2014
    Applicant: BIOO SCIENTIFIC CORPORATION
    Inventors: Masoud M. Toloue, Jason Dickson, Prachi Nakashe, Marianna Goldrick
  • OLIGONUCLEOTIDE LIGATION, BARCODING AND METHODS AND COMPOSITIONS FOR IMPROVING DATA QUALITY AND THROUGHPUT USING MASSIVELY PARALLEL SEQUENCING
    Publication number: 20120028814
    Abstract: Described herein is a buffer concentration for highly efficient ligation of two oligonucleotides. The embodiments herein have led to the development of an optimized ligation step used in the sample preparation for sequencing reactions. Further, embodiments herein describe a high-throughput method for sequencing using barcodes or the purpose of multiplexing several samples simultaneously and novel methods for making targeted DNA libraries for re-sequencing on massively parallel next-generation sequencing platforms and for alternatives to gel-purification for recovering the desired templates from small RNA libraries for next generation sequencing.
    Type: Application
    Filed: May 6, 2011
    Publication date: February 2, 2012
    Inventors: Masoud M. Toloue, Marianna Goldrick