Abstract: A method for analysis of biological samples, implemented in a reversed open microwell system which includes an array of open microwells, a microchannel, an input port for reagents and/or biological samples and an output port. The ports are in microfluidic communication with the microchannel. The microchannel has a cross-section area of micrometric dimensions and provides fluid to the microwells. The microwell system is inserted in an automated management system that includes: an incubator at controlled temperature, humidity and CO2, fluid dispensing system, phase-contrast and fluorescence image acquisition. A kit introduces fluids in a microfluidic device that includes a tip. A microfluidic device and system include a microchannel and an input region having a vertical channel. The tip and the vertical channel are dimensioned to produce an interference coupling. A discharge region includes: a discharge container connected with the microfluidic device through a discharge channel and an output port.

Abstract: The present invention relates to a method for subjecting a plurality of microwells containing cells to a high-content assay, said method comprising: a) Acquiring at least one image of said plurality of microwells; b) In said image, detecting a plurality of areas of interest, each area of interest corresponding to a single cell; c) Measuring at least one derived property, and, optionally, at least one direct property of said areas of interest, where said one or more properties is a selection property; d) Selecting a subset of said plurality of microwells, where said microwells belonging to the subset contain areas of interest selected based on said at least one selection property; e) Extrapolating an output parameter from a property measured in the set of areas of interest selected, where said property is defined as output property, said output property being distinct from said selection properties where said output parameter is the processing of an output property measured in said set of areas of interest.

Abstract: Methods are provided for the large-scale high-content analysis of biological samples. In some embodiments, the methods are implemented in a reversed open microwell system that includes an array of open microwells, at least one microchannel, at least one input port and at least one output port. In certain embodiments, the reversed open microwell system can be inserted in an automated management system which includes an incubator at controlled temperature, humidity and CO2 levels, a fluid dispensing system, and is capable of phase-contrast and fluorescence image acquisition. Also provided are kits for introducing fluids into a microfluidic device, and systems for discharging one or more fluids in a microfluidic device.

Abstract: Methods and systems for rapid and efficient screening of monoclonal antibodies and antibody-secreting cells, and particularly of single antibody-secreting cells, for both primary and functional characteristics, and particularly cell to cell interactions, in a microfluidic system, in particular an inverted open microwell system, where the particle(s) is not bound to a substrate.

Abstract: A method for analysis of biological samples, implemented in a reversed open microwell system which includes an array of open microwells, a microchannel, an input port for reagents and/or biological samples and an output port. The ports are in microfluidic communication with the microchannel. The microchannel has a cross-section area of micrometric dimensions and provides fluid to the microwells. The microwell system is inserted in an automated management system that includes: an incubator at controlled temperature, humidity and CO2, fluid dispensing system, phase-contrast and fluorescence image acquisition. A kit introduces fluids in a microfluidic device that includes a tip. A microfluidic device and system include a microchannel and an input region having a vertical channel. The tip and the vertical channel are dimensioned to produce an interference coupling. A discharge region includes: a discharge container connected with the microfluidic device through a discharge channel and an output port.

Abstract: An inverted microwell provides rapid and efficient microanalysis system and method for screening of biological particles, particularly functional analysis of cells on a single cell basis. The use of an inverted open microwell system permits identification of particles, cells, and biomolecules that may be combined to produce a desired functional effect also functional screening of secreted antibody therapeutic activity as well as the potential to recover cells and fluid, and optionally expand cells, such as antibody secreting cells, within the same microwell.

Abstract: An inverted microwell provides rapid and efficient microanalysis system and method for screening of biological particles, particularly functional analysis of cells on a single cell basis. The use of an inverted open microwell system permits identification of particles, cells, and biomolecules that may be combined to produce a desired functional effect also functional screening of secreted antibody therapeutic activity as well as the potential to recover cells and fluid, and optionally expand cells, such as antibody secreting cells, within the same microwell.

Abstract: Methods are provided for the large-scale high-content analysis of biological samples. In some embodiments, the methods are implemented in a reversed open microwell system that includes an array of open microwells, at least one microchannel, at least one input port and at least one output port In certain embodiments, the reversed open microwell system can be inserted in an automated management system which includes an incubator at controlled temperature, humidity and CO2 levels, a fluid dispensing system, and is capable of phase-contrast and fluorescence image acquisition. Also provided are kits for introducing fluids into a microfluidic device and systems for discharging one or more fluids in a microfluidic device.

Abstract: An inverted microwell (102) provides rapid and efficient microanalysis system (100) and method for screening of biological particles (128), particularly functional analysis of cells on a single cell basis. The use of an inverted open microwell system (102) permits identification of particles, cells, and biomolecules that may be combined to produce a desired functional effect also functional screening of secreted antibody therapeutic activity as well as the potential to recover cells and fluid, and optionally expand cells, such as antibody secreting cells, within the same microwell.

Abstract: An inverted microwell provides rapid and efficient microanalysis system and method for screening of biological particles, particularly functional analysis of cells on a single cell basis. The use of an inverted open microwell system permits identification of particles, cells, and biomolecules that may be combined to produce a desired functional effect also functional screening of secreted antibody therapeutic activity as well as the potential to recover cells and fluid, and optionally expand cells, such as antibody secreting cells, within the same microwell.

Abstract: An inverted microwell provides rapid and efficient microanalysis system and method for screening of biological particles, particularly functional analysis of cells on a single cell basis. The use of an inverted open microwell system permits identification of particles, cells, and biomolecules that may be combined to produce a desired functional effect also functional screening of secreted antibody therapeutic activity as well as the potential to recover cells and fluid, and optionally expand cells, such as antibody secreting cells, within the same microwell.

Abstract: A method for using a well, in particular a well open at an upper end and having a vertical axis, able to contain a liquid and particles contained in said liquid, includes the use of said well in a normal configuration with said upper end facing upwards, and in an overturned configuration with said upper end facing downwards in which a liquid is retained in the well by capillarity.

Abstract: A well, in particular an open well (14) with an upper end having a vertical axis (101), for containing a liquid and particles contained within said liquid, characterized by comprising at least two manipulation electrodes (1, 2, 3, 31, 32, 36, 17, 40, 41) able to be powered by electrical voltages, in particular alternating electrical voltages, so as to manoeuvre particles within the well by means of the dielectrophoretic effect. A platform comprising a plurality of wells as described above and a method for using said well.

Abstract: An inverted microwell (102) provides rapid and efficient microanalysis system (100) and method for screening of biological particles (128), particularly functional analysis of cells on a single cell basis. The use of an inverted open microwell system (102) permits identification of particles, cells, and biomolecules that may be combined to produce a desired functional effect also functional screening of secreted antibody therapeutic activity as well as the potential to recover cells and fluid, and optionally expand cells, such as antibody secreting cells, within the same microwell.

Abstract: Methods and systems for rapid and efficient screening of monoclonal antibodies and antibody-secreting cells, and particularly of single antibody-secreting cells, for both primary and functional characteristics, and particularly cell to cell interactions, in a microfluidic system, in particular an inverted open microwell system, where the particle(s) is not bound to a substrate.

Abstract: A well, in particular an open well (14) with an upper end having a vertical axis (101), for containing a liquid and particles contained within said liquid, characterized by comprising at least two manipulation electrodes (1, 2, 3, 31, 32, 36, 17, 40, 41) able to be powered by electrical voltages, in particular alternating electrical voltages, so as to manoeuvre particles within the well by means of the dielectrophoretic effect. A platform comprising a plurality of wells as described above and a method for using said well.