Abstract: A cell modified for obtaining increased proliferative capacity, decreased aging and enhanced regenerative capacity, its modification involving HOXB7 overexpression obtained using a gene vector that can insert the coding sequence into the cell, thereby affording increased protein production.

Abstract: Method for the production of bi-functional cells comprising engineering a starting cell population with a phenotype attributable to human pericytes extracted from adipose tissue (AD-PC), obtaining engineered cells, known as bi-functional AD-PCs, expressing (which means that they produce) simultaneously both the anti-tumor molecule TRAIL and also the truncated form of a chimeric receptor targeted against the GD2 antigen (GD2 tCAR); this dual targeting (understood as reaching a specific target), based on affinity and mediated by both TRAIL and also GD2 tCAR, supports the prediction of combining site-specificity with a prolonged retention of AD-PCs in tumors expressing the antigen GD2, so as to achieve a more effective release of TRAIL for still incurable tumors.

Abstract: The method to produce conditionally apoptotic cells comprises modifying a cell population with a phenotype attributable to human adipose tissue-derived pericytes (AD-PC) expressing anti-tumor TRAIL introducing a sequence coding a conditional suicide gene (CSG).

Abstract: Method for the production of cellular particulate with antitumor activity, which comprises isolating the cellular particulate originating from a cell population with a phenotype that can be attributed to human adipose tissue derived pericytes (AD-PC) that express antitumor TRAIL.

Abstract: Three-dimensional cell culture device comprising: a container body of cells to be cultivated formed by a first semi-portion and a second semi-portion facing each other and attached together with attachment means; a culture compartment which is defined between the first semi-portion and the second semi-portion; a three-dimensional substrate for the engraftment and/or support of the cells to be cultivated which is located in the culture compartment; an inlet of a transport solution of cells to be cultivated, and an outlet that connects the culture compartment with the outside, to discharge the transport solution; at least one of said first semi-portion and second semi-portion comprises oxygenation means of the cells to be cultivated.

Abstract: The present invention relates to mesenchymal stromal cells and to an advantageous method for their isolation from umbilical cord. In particular, the cells are obtainable by a method comprising isolating the stromal Wharton's Jelly or a fraction thereof from an umbilical cord and subjecting the stromal Wharton's Jelly or fraction thereof to enzymatic treatment, thereby releasing at least one cell from the stromal Wharton's Jelly or fraction thereof. Preferably, cultivation of cells occurs under conditions allowing the expansion of the cell population. The cell population according to the present invention has proliferative properties and is of industrial interest for a variety of purposes.

Abstract: A modified effector cell includes a non-reversibly produced vector-encoded anti-GD2-BB-? chimeric receptor for use in disialoganglioside GD2-expressing neoplasia, which is inserted in the cell, to obtain an effector cell that stably produces the anti-GD2-BB-? chimeric receptor, the chimeric receptor having two distinct mutually fused portions, i.e. an intra-cytoplasmic portion and an extra-cytoplasmic portion.

Abstract: A method of obtaining stromal progenitor cells (SPC) from subcutaneous adipose tissue by incubation of a very small volume of the subcutaneous adipose tissue in an enzyme solution produces SPC that are usable in medical applications based on autologous SPC even on individuals having a body mass index lower than 18.5.

Abstract: A cell modified for obtaining increased proliferative capacity, decreased aging and enhanced regenerative capacity, its modification involving HOXB7 overexpression obtained using a gene vector that can insert the coding sequence into the cell, thereby affording increased protein production.

Abstract: A method for production of anti-tumor TRAIL includes inserting a TRAIL molecule, encoded by a viral vector irreversibly derived from a cell line, into a carrier cell, thereby obtaining a stably TRAIL-producing carrier cell, and wherein the TRAIL molecule includes a soluble molecule.

Abstract: A method for production of anti-tumor TRAIL includes inserting a TRAIL molecule, encoded by a viral vector irreversibly derived from a cell line, into a carrier cell, thereby obtaining a stably TRAIL-producing carrier cell, and wherein the TRAIL molecule includes a soluble molecule.

Abstract: A device for cell culture includes a container body, which has an internal compartment to contain a quantity of cells to be cultured and a culture surface for the cells placed in the internal compartment; an introduction opening for introducing the cells to be cultured in the internal compartment; the container body having a first half shell and a second half shell, which can be fixed in mutually facing relation by a removable fixing system and which together define the internal compartment and the culture surface; and at least one out feed opening for a transport and/or culture fluid for the cells.

Abstract: A modified effector cell includes a non-reversibly produced vector-encoded anti-GD2-BB-? chimeric receptor for use in disialoganglioside GD2-expressing neoplasia, which is inserted in the cell, to obtain an effector cell that stably produces the anti-GD2-BB-? chimeric receptor, the chimeric receptor having two distinct mutually fused portions, i.e. an intra-cytoplasmic portion and an extra-cytoplasmic portion.

Abstract: A method of obtaining stromal progenitor cells (SPC) from subcutaneous adipose tissue by incubation of a very small volume of the subcutaneous adipose tissue in an enzyme solution produces SPC that are usable in medical applications based on autologous SPC even on individuals having a body mass index lower than 18.5.

Abstract: The present invention relates to a technique for obtaining stromal progenitor cells (SPC) from adipose tissue (AT) using incubation of very small volumes of AT with an enzyme solution, that can obtain SPC for autologous SPC-based medical applications to a greater number of individuals, possibly having a lower Body Mass Index (B.M.I), i.e. lower than 18.5.

Abstract: The method for production of anti-tumor TRAIL comprises: inserting a TRAIL molecule, encoded by a viral vector irreversibly derived from a cell line, into a carrier cell, thereby obtaining a stably TRAIL-producing carrier cell, said TRAIL molecule comprising a soluble molecule.

Abstract: The device for cell culture comprises: a container body (2) which has an internal compartment (5) to contain a quantity of cells to be cultured and a culture surface (3A; 120) for said cells placed in said internal compartment (5); an introduction opening (6; 106) for introducing said cells to be cultured in said compartment (5); said container body (2) comprising: first half shell means (3; 103) and second half shell means (4; 104) which can be fixed in mutually facing relation by removable fixing means (8; 108, 108?) and which together define said internal compartment (5) and said culture surface (3A; 120); at least one out feed opening (7; 107) for a transport and/or culture fluid for said cells.