Patents by Inventor Masuo Obinata
Masuo Obinata has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 7964367Abstract: Novel human genes falling within the category of family genes relating to p53 gene which is known as a cell proliferation regulatory gene, and gene products thereof. A human p51 gene characterized by containing a base sequence encoding an amino acid sequence represented by SEQ ID NO:1; a human p51 gene having a base sequence consisting of the 145- to 1488-bases in the sequence represented by SEQ ID NO:2; vectors containing these genes; host cells transformed with these vectors; a process for producing a p51 protein having the amino sequence represented by SEQ ID NO:1; which comprises culturing the above host cells and harvesting the protein from the thus obtained culture; and the p51 protein having the amino acid sequence represented by SEQ ID NO:1.Type: GrantFiled: May 24, 2010Date of Patent: June 21, 2011Assignees: Otsuka Pharmaceutical Co., Ltd.Inventors: Yoji Ikawa, Shuntaro Ikawa, Masuo Obinata
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Publication number: 20100285499Abstract: Novel human genes falling within the category of family genes relating to p53 gene which is known as a cell proliferation regulatory gene, and gene products thereof. A human p51 gene characterized by containing a base sequence encoding an amino acid sequence represented by SEQ ID NO:1; a human p51 gene having a base sequence consisting of the 145- to 1488-bases in the sequence represented by SEQ ID NO:2; vectors containing these genes; host cells transformed with these vectors; a process for producing a p51 protein having the amino sequence represented by SEQ ID NO:1; which comprises culturing the above host cells and harvesting the protein from the thus obtained culture; and the p51 protein having the amino acid sequence represented by SEQ ID NO:1.Type: ApplicationFiled: May 24, 2010Publication date: November 11, 2010Applicants: OTSUKA PHARMACEUTICAL CO., LTD.Inventors: Yoji IKAWA, Shuntaro IKAWA, Masuo OBINATA
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Patent number: 7754857Abstract: Novel human genes falling within the category of family genes relating to p53 gene which is known as a cell proliferation regulatory gene, and gene products thereof. A human p51 gene characterized by containing a base sequence encoding an amino acid sequence represented by SEQ ID NO:1; a human p51 gene having a base sequence consisting of the 145- to 1488-bases in the sequence represented by SEQ ID NO:2; vectors containing these genes; host cells transformed with these vectors; a process for producing a p51 protein having the amino sequence represented by SEQ ID NO:1; which comprises culturing the above host cells and harvesting the protein from the thus obtained culture; and the p51 protein having the amino acid sequence represented by SEQ ID NO:1.Type: GrantFiled: July 8, 2008Date of Patent: July 13, 2010Assignees: Otsuka Pharmaceutical Co., Ltd.Inventors: Yoji Ikawa, Shuntaro Ikawa, Masuo Obinata
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Publication number: 20100167397Abstract: In accordance with the present invention, there are provided (1) various cell lines derived from hypothalamus and Langerhans islets of mammals, (2) process for producing an active peptide and expression cloning system of active peptide precursor gene using the cell line as a host, (3) a method of screening or evaluating a substance capable of acting on the cells using the cell line, (4) a method of screening or isolating a useful gene or useful peptide using the cell line and (5) a highly-sensitive and simple assay system for GPCR ligand used in the above expression cloning system.Type: ApplicationFiled: September 18, 2009Publication date: July 1, 2010Applicant: KYOWA HAKKO KOGYO CO., LTD.Inventors: KATSUTOSHI SASAKI, KAZUMI MIURA, SATOSHI SAEKI, MISAKO SUZUKI, KAZUYA KISHIMOTO, HIROFUMI KUNITOMO, TATSUNARI NISHI, MASUO OBINATA
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Patent number: 7553933Abstract: Novel human genes falling within the category of family genes relating to p53 gene which is known as a cell proliferation regulatory gene, and gene products thereof. A human p51 gene characterized by containing a base sequence encoding an amino acid sequence represented by SEQ ID NO:1; a human p51 gene having a base sequence consisting of the 145- to 1488-bases in the sequence represented by SEQ ID NO:2; vectors containing these genes; host cells transformed with these vectors; a process for producing a p51 protein having the amino sequence represented by SEQ ID NO:1; which comprises culturing the above host cells and harvesting the protein from the thus obtained culture; and the p51 protein having the amino acid sequence represented by SEQ ID NO:1.Type: GrantFiled: January 27, 2006Date of Patent: June 30, 2009Assignees: Otsuka Pharmaceutical Co., Ltd.Inventors: Yoji Ikawa, Shuntaro Ikawa, Masuo Obinata
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Publication number: 20090142783Abstract: Novel human genes falling within the category of family genes relating to p53 gene which is known as a cell proliferation regulatory gene, and gene products thereof. A human p51 gene characterized by containing a base sequence encoding an amino acid sequence represented by SEQ ID NO:1; a human p51 gene having a base sequence consisting of the 145- to 1488-bases in the sequence represented by SEQ ID NO:2; vectors containing these genes; host cells transformed with these vectors; a process for producing a p51 protein having the amino sequence represented by SEQ ID NO:1; which comprises culturing the above host cells and harvesting the protein from the thus obtained culture; and the p51 protein having the amino acid sequence represented by SEQ ID NO:1.Type: ApplicationFiled: July 8, 2008Publication date: June 4, 2009Applicants: OTSUKA PHARMACEUTICAL CO., LTD., IKAWA, YOJIInventors: Yoji IKAWA, Shuntaro Ikawa, Masuo Obinata
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Patent number: 7316926Abstract: To provide an immortalized capillary pericyte line which maintains the original function/property of the cell line-deriving tissue, its establishment method, and the screening method for useful substance using the immortalized capillary pericyte line. Cerebral tissue of a transgenic rat carrying the large T antigen gene of SV40 thermo-sensitive mutant line tsA58 is homogenized and the resultant brain capillaries are treated with protease, thus obtained brain capillary cells are subcultured to establish an immortalized cell that expresses SV40 thermo-sensitive large T antigen, PDGF receptor ?, and Angiopoietin-1. In addition, the immortalized vascular pericyte line has ability to deposit calcium on matrix by dense culture.Type: GrantFiled: February 14, 2001Date of Patent: January 8, 2008Assignee: Japan Science and Technology AgencyInventors: Hisashi Iizasa, Kenji Hattori, Emi Nakashima, Tetsuya Terasaki, Masuo Obinata
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Publication number: 20070105096Abstract: In accordance with the present invention, there are provided (1) various cell lines derived from hypothalamus and Langerhans islets of mammals, (2) process for producing an active peptide and expression cloning system of active peptide precursor gene using the cell line as a host, (3) a method of screening or evaluating a substance capable of acting on the cells using the cell line, (4) a method of screening or isolating a useful gene or useful peptide using the cell line and (5) a highly-sensitive and simple assay system for GPCR ligand used in the above expression cloning system.Type: ApplicationFiled: April 16, 2003Publication date: May 10, 2007Inventors: Katsutoshi Sasaki, Kazumi Miura, Satoshi Saeki, Misako Suzuki, Kazuya Kishimoto, Hirofumi Kunitomo, Tatsunari Nishi, Masuo Obinata
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Publication number: 20070104679Abstract: This invention provides a method to induce the differentiation of animal cells wherein said cells are brought into contact with cytokine only in a suitable growth phase of animal cells, and also provides a system to control the differentiation potency of animal cells wherein two or more cytokines are combined for use in the cultivation or growing of animal cells.Type: ApplicationFiled: March 24, 2004Publication date: May 10, 2007Inventors: Masuo Obinata, Yoshihisa Suzuki
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Publication number: 20070037178Abstract: In accordance with the present invention, there are provided (1) various cell lines derived from hypothalamus and Langerhans islets of mammals, (2) process for producing an active peptide and expression cloning system of active peptide precursor gene using the cell line as a host, (3) a method of screening or evaluating a substance capable of acting on the cells using the cell line, (4) a method of screening or isolating a useful gene or useful peptide using the cell line and (5) a highly-sensitive and simple assay system for GPCR ligand used in the above expression cloning system.Type: ApplicationFiled: January 17, 2006Publication date: February 15, 2007Applicant: KYOWA HAKKO KOGYO CO., LTD.Inventors: Katsutoshi Sasaki, Kazumi Miura, Satoshi Saeki, Misako Suzuki, Kazuya Kishimoto, Hirofumi Kunitomo, Tatsunari Nishi, Masuo Obinata
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Patent number: 7132276Abstract: Novel human genes falling whithin the category of family genes relating to p53 gene which is known as a cell proliferation regulatory gene, and gene products thereof. A human p51 gene characterized by containing a base sequence encoding an amino acid sequence represented by SEQ ID NO:1; a human p51 gene having a base sequence consisting of the 145- to 1488-bases in the sequence represented by SEQ ID NO:2; vectors containing these genes; host cells transformed with these vectors; a process for producing a p51 protein having the amino sequence represented by SEQ ID NO:1; which comprises culturing the above host cells and harvesting the protein from the thus obtained culture; and the p51 protein having the amino acid sequence represented by SEQ ID NO:1.Type: GrantFiled: March 24, 1999Date of Patent: November 7, 2006Assignees: Otsuka Pharmaceutical Co., LtdInventors: Yoji Ikawa, Shuntaro Ikawa, Masuo Obinata
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Publication number: 20060115852Abstract: Novel human genes falling within the category of family genes relating to p53 gene which is known as a cell proliferation regulatory gene, and gene products thereof. A human p51 gene characterized by containing a base sequence encoding an amino acid sequence represented by SEQ ID NO:1; a human p51 gene having a base sequence consisting of the 145- to 1488-bases in the sequence represented by SEQ ID NO:2; vectors containing these genes; host cells transformed with these vectors; a process for producing a p51 protein having the amino sequence represented by SEQ ID NO:1; which comprises culturing the above host cells and harvesting the protein from the thus obtained culture; and the p51 protein having the amino acid sequence represented by SEQ ID NO:1.Type: ApplicationFiled: January 27, 2006Publication date: June 1, 2006Inventors: Yoji Ikawa, Shuntaro Ikawa, Masuo Obinata
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Publication number: 20060067914Abstract: The present invention provides an immortalized natural killer cell line retaining the function and characteristics intrinsic to natural killer cells, a method for establishing the same, a method for screening for useful substances using the immortalized natural killer cell line, and a cell vaccine. By culturing natural killer cells obtained by isolating natural killer cells from the spleen of a transgenic mouse to which a large T-antigen gene of SV40 temperature-sensitive mutant tsA58 is introduced, a cell line which proliferates and activates in the presence of Interleukin-2, has azurophilic granules within cytoplasm, and retains an ability to kill a target cell without presensitization and/or an ability to kill target cells coated with an antibody, is established.Type: ApplicationFiled: April 26, 2005Publication date: March 30, 2006Inventors: Satoru Iizuka, Toshiyuki Takai, Yumi Ito, Kozue Ito, Masuo Obinata
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Publication number: 20030175953Abstract: To provide an immortalized capillary pericyte line which maintains the original function/property of the cell line-deriving tissue, its establishment method, and the screening method for useful substance using the immortalized capillary pericyte line. Cerebral tissue of a transgenic rat carrying the large T antigen gene of SV40 thermo-sensitive mutant line tsA58 is homogenized and the resultant brain capillaries are treated with protease, thus obtained brain capillary cells are subcultured to establish an immortalized cell that expresses SV40 thermo-sensitive large T antigen, PDGF receptor &bgr;, and Angiopoietin-1. In addition, the immortalized vascular pericyte line has ability to deposit calcium on matrix by dense culture.Type: ApplicationFiled: August 16, 2002Publication date: September 18, 2003Inventors: Hisashi Iizasa, Kenji Hattori, Emi Nakashima, Tetsuya Terasaki, Masuo Obinata
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Publication number: 20020045254Abstract: Established cells derived from retinal capillary endothelial cells, choroid plexus epithelial cells, or brain capillary endothelial cells or a transgenic animal carrying a large T-antigen gene of an SV40 temperature sensitive mutant tsA58. The cell line derived from retinal capillary endothelial cells expresses a temperature sensitive SV40 large T-antigen, GLUT-1 transporter, and p-glycoprotein. The cell line derived from choroid plexus epithelial cells expresses a temperature sensitive SV40 large T-antigen gene and shows localization of Na+—K+ ATPase and GLUT-1 transporter in the cell membrane. When cultured in a monolayer, it shows the localization of Na+—K+ ATPase in the apical side. The cell line derived from brain capillary endothelial cells expresses a temperature sensitive SV40 large T-antigen, GLUT-1 transporter, p-glycoprotein, alkaline phosphatase, and &ggr;-glutamyltransferase.Type: ApplicationFiled: March 29, 2001Publication date: April 18, 2002Inventors: Kenichi Hosoya, Tetsuya Terasaki, Masatsugu Ueda, Masuo Obinata
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Patent number: 5776446Abstract: Lymphotoxin-producing human T-cell hybridomas are incubated in a medium containing phorbol myristate acetate, concanavalin-A or a mixture thereof, the resulting cells are fractionated by sucrose density-gradient centrifugation method to isolate a messenger RNA in 12.6S to 14.6S fractions, and a gene containing a part encoding a polypeptide having a lymphotoxin-activity is prepared from the messenger RNA. The gene is represented by the base sequence of the Table 1 as herein given. Using the present gene, a new lymphotoxin having the amino acid sequence (I) or (II) as given herein can be obtained by genetic engineering technology.Type: GrantFiled: May 16, 1994Date of Patent: July 7, 1998Assignee: Denki Kagaku Kogyo Kabushiki KaishaInventors: Toshiaki Osawa, Masuo Obinata, Yoshiyuki Ishii, Yoshio Kobayashi
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Patent number: 5403725Abstract: Lymphotoxin-producing human T-cell hybridomas are incubated in a medium containing phorbol myristate acetate, concanavalin-A or a mixture thereof, the resulting cells are fractionated by sucrose density-gradient centrifugation method to isolate a messenger RNA in 12.6S to 14.6S fractions, and a gene containing a part encoding a polypeptide having a lymphotoxin-activity is prepared from the messenger RNA. The gene is represented by the nucleotide sequence of the Table 1 as herein given. Using the present gene, a new lymphotoxin having the amino acid sequence (I) or (II) as given herein can be obtained by genetic engineering technology.Type: GrantFiled: July 22, 1991Date of Patent: April 4, 1995Assignee: Denki Kagaku Kogyo Kabushiki KaishaInventors: Toshiaki Osawa, Masuo Obinata, Yoshiyuki Ishii, Yoshio Kobayashi