Abstract: The present disclosure relates to engineered DNA polymerase polypeptides and compositions thereof, as well as polynucleotides encoding the engineered DNA polymerase polypeptides. The present disclosure also provides methods of using the engineered DNA polymerase polypeptides or compositions thereof for diagnostic and other purposes.

Abstract: This invention relates to methods of selecting plant-associated bacterial isolates for promoting plant health.

Abstract: Pump systems with pinch valves for surgical procedures. At least some of the example embodiments are pump systems including a stationary housing defining a front face. A tube support extends outwardly from the front face for aligning and supporting a first tube and a second tube. At least one pinch member is movable relative to the tube support by a power actuator operably coupled to the at least one pinch member and configured to have three orientations that define: a first arrangement of the pinch member relative to the tube support configured to pinch closed the first tube, a second arrangement of the at least one pinch member relative to the tube support configured to pinch closed the second tube, and a third arrangement of the at least one pinch member relative to the tube support configured to pinch closed neither the first tube nor the second tube.

Abstract: Pump systems with pinch valves for surgical procedures. At least some of the example embodiments are pump systems including a stationary housing defining a front face. A tube support extends outwardly from the front face for aligning and supporting a first tube and a second tube. At least one pinch member is movable relative to the tube support by a power actuator operably coupled to the at least one pinch member and configured to have three orientations that define: a first arrangement of the pinch member relative to the tube support configured to pinch closed the first tube, a second arrangement of the at least one pinch member relative to the tube support configured to pinch closed the second tube, and a third arrangement of the at least one pinch member relative to the tube support configured to pinch closed neither the first tube nor the second tube.

Abstract: Pump systems with pinch valves for surgical procedures. At least some of the example embodiments are pump systems including a stationary housing defining a front face. A tube support extends outwardly from the front face for aligning and supporting a first tube and a second tube. At least one pinch member is movable relative to the tube support by a power actuator operably coupled to the at least one pinch member and configured to have three orientations that define: a first arrangement of the pinch member relative to the tube support configured to pinch closed the first tube, a second arrangement of the at least one pinch member relative to the tube support configured to pinch closed the second tube, and a third arrangement of the at least one pinch member relative to the tube support configured to pinch closed neither the first tube nor the second tube.

Abstract: Pump systems with pinch valves for surgical procedures. At least some of the example embodiments are pump systems including a stationary housing defining a front face. A tube support extends outwardly from the front face for aligning and supporting a first tube and a second tube. At least one pinch member is movable relative to the tube support by a power actuator operably coupled to the at least one pinch member and configured to have three orientations that define: a first arrangement of the pinch member relative to the tube support configured to pinch closed the first tube, a second arrangement of the at least one pinch member relative to the tube support configured to pinch closed the second tube, and a third arrangement of the at least one pinch member relative to the tube support configured to pinch closed neither the first tube nor the second tube.

Abstract: Pump systems with pinch valves for surgical procedures. At least some of the example embodiments are pump systems including a stationary housing defining a front face. A tube support extends outwardly from the front face for aligning and supporting a first tube and a second tube. At least one pinch member is movable relative to the tube support by a power actuator operably coupled to the at least one pinch member and configured to have three orientations that define: a first arrangement of the pinch member relative to the tube support configured to pinch closed the first tube, a second arrangement of the at least one pinch member relative to the tube support configured to pinch closed the second tube, and a third arrangement of the at least one pinch member relative to the tube support configured to pinch closed neither the first tube nor the second tube.

Abstract: Pump systems with pinch valves for surgical procedures. At least some of the example embodiments are pump systems including a stationary housing defining a front face. A tube support extends outwardly from the front face for aligning and supporting a first tube and a second tube. At least one pinch member is movable relative to the tube support by a power actuator operably coupled to the at least one pinch member and configured to have three orientations that define: a first arrangement of the pinch member relative to the tube support configured to pinch closed the first tube, a second arrangement of the at least one pinch member relative to the tube support configured to pinch closed the second tube, and a third arrangement of the at least one pinch member relative to the tube support configured to pinch closed neither the first tube nor the second tube.

Abstract: A drive gear for a generally cylindrical imaging component. The drive gear includes a body for engaging the generally cylindrical imaging component having an axis of rotation, a drive portion extending out from body along the axis of rotation, wherein the drive portion comprises a plurality of pillars and narrows towards the axis of rotation of the drive gear as the distance from the end surface increases outward.

Abstract: A method and apparatus include determining a value of a parameter associated with operation of an electrosurgical probe having a particular probe design, and determining whether the value of the parameter is within a range of values that has been predetermined for the particular probe design to indicate that the probe is treating tissue in a desired manner. Power is delivered to the probe according to an algorithm based upon a determination that the value of the parameter is outside the range of values The algorithm delivers power in a pulsed profile including portions of low power and portions of high power. In one embodiment, the tissue treatment is ablation, the parameter is impedance, and the method limits tissue necrosis to less than 200 microns. In another embodiment, the tissue treatment is shrinkage, the parameter is temperature, and the method limits power delivery when the probe is not shrinking tissue.

Abstract: An electrosurgical apparatus includes a probe having a first electrode surface area for performing a first electrosurgical procedure and a second electrode surface area for performing a second electrosurgical procedure. The second electrosurgical procedure is different from the first electrosurgical procedure. The apparatus includes the second electrode surface area overlapping the first electrode surface area and/or a masking device operable to mask at least a portion of the first electrode surface area.

Abstract: A method and apparatus include determining a value of a parameter associated with operation of an electrosurgical probe having a particular probe design, and determining whether the value of the parameter is within a range of values that has been predetermined for the particular probe design to indicate that the probe is treating tissue in a desired manner. Power is delivered to the probe according to an algorithm based upon a determination that the value of the parameter is outside the range of values The algorithm delivers power in a pulsed profile including portions of low power and portions of high power. In one embodiment, the tissue treatment is ablation, the parameter is impedance, and the method limits tissue necrosis to less than 200 microns. In another embodiment, the tissue treatment is shrinkage, the parameter is temperature, and the method limits power delivery when the probe is not shrinking tissue.

Abstract: A laparoscopic bipolar electrosurgical instrument can apply a large closure force between its jaws without damaging the small yoke assembly.

Abstract: An electrosurgical instrument includes a shaft, a flexible portion, and a head coupled to the shaft through the flexible portion and pivotably coupled to the flexible portion. The head includes a non-conductive surface and an electrically conductive surface. The flexible portion is configured to bias the non-conductive surface and the electrically conductive surface towards a tissue surface, such as cartilage. The non-conductive surface may include a material having a thermal conductivity less than or equal to about 30 W/m*K and/or a volume resistivity greater than or equal to about 1×1014 ohm*cm. The non-conductive surface may include a ceramic such as Macor® ceramic, ZTA ceramic, and/or 99.5% alumina ceramic.

Abstract: A bipolar electrosurgical instrument for clamping, grasping, manipulating, and sealing tissue includes first and second shafts each having a jaw member extending from a distal end thereof and a handle disposed at a proximal end thereof. The handle being operable to effect movement of the jaw members relative to one another from a first position wherein the jaw members are disposed in spaced relation relative to one another to a second position wherein the jaw members cooperate to grasp tissue therebetween. The bipolar instrument is connectable to a source of electrical energy having a first electrical potential connected to one of the jaw members and a second electrical potential connected to the other of the jaw members such that the jaw members are capable of selectively conducting energy through tissue held therebetween to effect a seal. Both the first and second electrical potentials are transmitted to the jaw members through the first shaft.