Abstract: The present invention relates to the targeted regulation of gene expression and more specifically to synthetic transcription factors (STFs) comprising at least one highly target specific engineered recognition domain and further comprising at least one activation or silencing domain to modulate the expression of a gene of interest, preferably to modulate the transcription of a morphogenic gene of a eukaryote. Further disclosed are methods using the STFs to enhance transformation frequencies, to optimize successful genome editing approaches, to provide haploid or double haploid organisms, and/or to provide compositions suitable for general transformation, but also for breeding purposes.

Abstract: The present invention relates to methods for targeted editing in a plant, a plant cell or material, which is combined with the parallel introduction of a phenotypically selectable trait. Furthermore, methods are provided not comprising a step of introducing a transgenic selection marker sequence.

Abstract: The present invention relates to the targeted regulation of gene expression and more specifically to synthetic transcription factors (STFs) comprising at least one highly target specific engineered recognition domain based on a CRISPR/Cpf1 system and further comprising at least one activation or silencing domain to modulate the expression of a gene of interest, preferably to modulate the transcription of a morphogenic gene of a eukaryote, in particular a plant. Further disclosed are methods using the STFs to enhance transformation frequencies, to optimize successful genome editing approaches, to provide haploid or double haploid organisms, and/or to provide compositions suitable for general transformation, but also for breeding purposes.

Abstract: The present invention relates to the targeted regulation of gene expression and more specifically to synthetic transcription factors (STFs) comprising at least one highly target specific engineered recognition domain and further comprising at least one activation or silencing domain to modulate the expression of a gene of interest, preferably to modulate the transcription of a morphogenic gene of a eukaryote. Further disclosed are methods using the STFs to enhance transformation frequencies, to optimize successful genome editing approaches, to provide haploid or double haploid organisms, and/or to provide compositions suitable for general transformation, but also for breeding purposes.

Abstract: The present invention relates to artificial molecular complexes comprising at least one site-specific nuclease and directly in interacting therewith at least one repair template docking domain, said repair template docking domain interacting with at least one repair template nucleic acid sequence. The artificial complex can further comprise at least one interaction domain. The artificial molecular complexes are configured to mediate repair of a DNA target sequence in a prokaryotic or eukaryotic organism with high precision in a targeted way and can thus be used for genome engineering in a prokaryotic or a eukaryotic cell or organism, or editing of a viral genome. Further provided are methods of modifying at least one DNA target sequence in a prokaryotic or eukaryotic cell or a viral genome, e.g., for trait development, or for treating a disease.