Abstract: The present invention belongs to the field of biotechnology, specifically to the field of recombinant protein expression. The present invention focuses on two problems commonly encountered during recombinant protein expression, low quantity of protein expression and genetic instability of cell lines used for recombinant protein expression. The basic principle of the present invention is to introduce several expression cassettes into a cell which expression cassettes all code for the same mature recombinant protein of interest, but which expression cassettes have different nucleotide sequences. Expression cassette means a polynucleotide sequence which comprises at least a promoter sequence, a start codon, a polynucleotide sequence coding for a protein which is intended to be recombinant expressed (POI), a stop codon and a terminator.

Abstract: The present disclosure provides improved methods for purifying recombinant protein, vaccine and gene therapy preparations, such as vectors in a suspension, as well as new means to better assay residual nucleic acids in a composition comprising genetically engineered particles. One aspect of the present disclosure is a method for purifying a liquid composition comprising genetically engineered particles from nucleic acid impurities comprising the steps of (i) adding a Dps protein to the suspension comprising genetically engineered particles, (ii) precipitating a complex comprising the nucleic acid impurities and the Dps protein and (iii) removing the precipitated complex comprising the nucleic acid impurities and Dps protein from the suspension comprising genetically engineered particles. Another aspect of the disclosure is a method to assay nucleic acid impurities in a liquid composition comprising genetically engineered particles or a pharmaceutical composition comprising genetically engineered particles.

Abstract: The present invention belongs to the field of biotechnology, specifically to the field of recombinant protein expression. The present invention focuses on two problems commonly encountered during recombinant protein expression, low quantity of protein expression and genetic instability of cell lines used for recombinant protein expression. The basic principle of the present invention is to introduce several expression cassettes into a cell which expression cassettes all code for the same mature recombinant protein of interest, but which expression cassettes have different nucleotide sequences. Expression cassette means a polynucleotide sequence which comprises at least a promoter sequence, a start codon, a polynucleotide sequence coding for a protein which is intended to be recombinant expressed (POI), a stop codon and a terminator.

Abstract: The present disclosure relates to a polynucleotide-binding protein for use in in vitro diagnostics. The present disclosure also relates to a method of separation of nucleic acids from a liquid biological sample, protecting nucleic acids in a biological sample from degradation, a method of extracting nucleic acids from a solution and a kit or container for protecting and/or separating nucleic acids from a solution, and more specifically to a method for preserving and/or separating nucleic acids from a sample.