Abstract: Methods are provided for determining a primer set for amplifying a target nucleic acid, as well as apparatuses and computer-readable storage media configured to perform aspects of the methods. In some cases, the methods include obtaining a first primer set; generating multiple child primer sets by performing modifications to the first primer set; and, for each of the child primer sets, determining a fitness score of the child primer set and, if the fitness score is at or above a predetermined threshold, determining the child primer set to be an acceptable primer set and adding the child primer set to a collection of acceptable primer sets stored in a memory device. The generating may generate at least some of the child primer sets in parallel. Multiple collections of acceptable primer sets may be generated in parallel. Various aspects of the methods may be controlled by a genetic algorithm.

Abstract: The present disclosure provides a guide wire system comprising (a) a guide wire having a distal end and a proximal end, wherein the guide wire comprises a superelastic material, (b) a first connector coupled to the proximal end of the guide wire, (c) a second connector coupled to the guide wire between the distal end and the proximal end, (d) an electromagnetic sensor coupled to the distal end of the guide wire, and (e) a polymeric tube surrounding the guide wire and at least a portion of the electromagnetic sensor.

Abstract: The present disclosure provides a guide wire system comprising (a) a guide wire having a distal end and a proximal end, wherein the guide wire comprises a superelastic material, (b) a first connector coupled to the proximal end of the guide wire, (c) a second connector coupled to the guide wire between the distal end and the proximal end, (d) an electromagnetic sensor coupled to the distal end of the guide wire, and (e) a polymeric tube surrounding the guide wire and at least a portion of the electromagnetic sensor.

Abstract: The present disclosure provides a guide wire system comprising (a) a guide wire having a distal end and a proximal end, wherein the guide wire comprises a superelastic material, (b) a first connector coupled to the proximal end of the guide wire, (c) a second connector coupled to the guide wire between the distal end and the proximal end, (d) an electromagnetic sensor coupled to the distal end of the guide wire, and (e) a polymeric tube surrounding the guide wire and at least a portion of the electromagnetic sensor.

Abstract: The present invention is directed towards systems and methods for enhanced marketing responsive to customer path data between attractions. A first device receives an identification of a location of a user and a first attraction selected for access by a user. A path analyzer of the device determines a travel path between the location of the user and the first attraction. The path analyzer identifies a second attraction within a predetermined distance from the travel path. The first device transmits, to a computing device of the user, an offer of access to the second attraction.

Abstract: The present invention is directed towards systems and methods for enhanced marketing responsive to customer path data between attractions. A first device receives an identification of a location of a user and a first attraction selected for access by a user. A path analyzer of the device determines a travel path between the location of the user and the first attraction. The path analyzer identifies a second attraction within a predetermined distance from the travel path. The first device transmits, to a computing device of the user, an offer of access to the second attraction.

Abstract: The present invention is directed towards systems and methods for validating an order purchased with an unspecified term. The order may comprise an order for an access ticket or admission ticket or access token, an order for goods or services, or any combination of access, goods, and services. Validation may comprise determining the ticket or token is valid and/or allowing access based on a determined data value for an access term unspecified at the time of purchase; or determining that the order is valid and fulfilling the order based on an identified data value for a term unspecified at the time of purchase. A data value may be set for the unspecified term and a hierarchical tree searched for a leaf corresponding to the set value for the unspecified term and one or more terms specified at time of purchase, the existence of the leaf indicating the order is valid.

Abstract: The present invention is directed towards systems and methods for improved access to an attraction via a computing device of a user. A first computing device of a user records an identification code associated with an attraction. The first computing device transmits, to a second computing device, a request for access to the attraction, the request comprising the identification code. The first computing device receives, from the second computing device, an access authorization comprising an access code. The first computing device presents the access code to an attraction operator for access to the attraction.

Abstract: The present invention is directed towards systems and methods for balancing a business margin against a target customer value to fulfill a request to purchase a plurality of items. An initial sale price for a request to purchase the items is calculated, the initial sale price based on a sum of retail prices for the items, discounted by a predetermined target customer value. An initial business margin for the request is determined, the initial business margin based on the sum of the retail prices for the items minus a sum of predetermined net prices for the items. The initial sale price is adjusted to create an intermediate sale price, responsive to the initial business margin being outside a range defined by a lower business margin threshold and an upper business margin threshold, and the intermediate sale price is compared to the initial sale price to create a final sale price.

Abstract: The present invention is directed towards systems and methods for improved access to an attraction via a computing device of a user. A first computing device of a user records an identification code associated with an attraction. The first computing device transmits, to a second computing device, a request for access to the attraction, the request comprising the identification code. The first computing device receives, from the second computing device, an access authorization comprising an access code. The first computing device presents the access code to an attraction operator for access to the attraction.

Abstract: The present invention is directed towards systems and methods for improved access to an attraction via a computing device of a user. A first computing device of a user records an identification code associated with an attraction. The first computing device transmits, to a second computing device, a request for access to the attraction, the request comprising the identification code. The first computing device receives, from the second computing device, an access authorization comprising an access code. The first computing device presents the access code to an attraction operator for access to the attraction.

Abstract: The present invention is directed towards systems and methods for validating an order purchased with an unspecified term. The order may comprise an order for an access ticket or admission ticket or access token, an order for goods or services, or any combination of access, goods, and services. Validation may comprise determining the ticket or token is valid and/or allowing access based on a determined data value for an access term unspecified at the time of purchase; or determining that the order is valid and fulfilling the order based on an identified data value for a term unspecified at the time of purchase. A data value may be set for the unspecified term and a hierarchical tree searched for a leaf corresponding to the set value for the unspecified term and one or more terms specified at time of purchase, the existence of the leaf indicating the order is valid.

Abstract: The present invention is directed towards systems and methods for validating an order purchased with an unspecified term. The order may comprise an order for an access ticket or admission ticket or access token, an order for goods or services, or any combination of access, goods, and services. Validation may comprise determining the ticket or token is valid and/or allowing access based on a determined data value for an access term unspecified at the time of purchase; or determining that the order is valid and fulfilling the order based on an identified data value for a term unspecified at the time of purchase. A data value may be set for the unspecified term and a hierarchical tree searched for a leaf corresponding to the set value for the unspecified term and one or more terms specified at time of purchase, the existence of the leaf indicating the order is valid.

Abstract: The present invention is directed towards systems and methods for improved access to an attraction via a computing device of a user. A first computing device of a user records an identification code associated with an attraction. The first computing device transmits, to a second computing device, a request for access to the attraction, the request comprising the identification code. The first computing device receives, from the second computing device, an access authorization comprising an access code. The first computing device presents the access code to an attraction operator for access to the attraction.

Abstract: The present invention is directed towards systems and methods for enhanced marketing responsive to customer path data between attractions. A first device receives an identification of a location of a user and a first attraction selected for access by a user. A path analyzer of the device determines a travel path between the location of the user and the first attraction. The path analyzer identifies a second attraction within a predetermined distance from the travel path. The first device transmits, to a computing device of the user, an offer of access to the second attraction.

Abstract: The present invention is directed towards systems and methods for balancing a business margin against a target customer value to fulfill a request to purchase a plurality of items. An initial sale price for a request to purchase the items is calculated, the initial sale price based on a sum of retail prices for the items, discounted by a predetermined target customer value. An initial business margin for the request is determined, the initial business margin based on the sum of the retail prices for the items minus a sum of predetermined net prices for the items. The initial sale price is adjusted to create an intermediate sale price, responsive to the initial business margin being outside a range defined by a lower business margin threshold and an upper business margin threshold, and the intermediate sale price is compared to the initial sale price to create a final sale price.

Abstract: The present invention is directed towards systems and methods for validating an order purchased with an unspecified term. The order may comprise an order for an access ticket or admission ticket or access token, an order for goods or services, or any combination of access, goods, and services. Validation may comprise determining the ticket or token is valid and/or allowing access based on a determined data value for an access term unspecified at the time of purchase; or determining that the order is valid and fulfilling the order based on an identified data value for a term unspecified at the time of purchase. A data value may be set for the unspecified term and a hierarchical tree searched for a leaf corresponding to the set value for the unspecified term and one or more terms specified at time of purchase, the existence of the leaf indicating the order is valid.