Abstract: Aerolysin polypeptides and/or mutant aerolysin monomers include modified amino acid sequences that could have improved substrate analyte, such as (poly)nucleotide and peptide, improved reading properties such as enhanced substrate analyte capture and improved substrate analyte recognition and/or discrimination. Also, aerolysin pores may be derived from the mutant monomers as well as apparatuses and devices may include modified aerolysin polypeptides. Further, methods of using modified aerolysin proteins and pores derive therefrom may be used in characterizing and/or sequencing a polymeric molecule or may be for use as molecular sensors.

Abstract: Systems and methods for data storage and readout using hybrid nucleic acid-polymeric molecules are herein disclosed. A molecular data storage medium is presented comprising a header and a footer, each comprising or consisting of at least one unit of a first chemical species; and a sequence-controlled polymeric chain of a second chemical species located between said header and said footer, said polymeric chain encoding for a desired bitstream-format media.

Abstract: Aerolysin polypeptides and/or mutant aerolysin monomers include modified amino acid sequences that could have improved substrate analyte, such as (poly)nucleotide and peptide, improved reading properties such as enhanced substrate analyte capture and improved substrate analyte recognition and/or discrimination. Also, aerolysin pores may be derived from the mutant monomers as well as apparatuses and devices may include modified aerolysin polypeptides. Further, methods of using modified aerolysin proteins and pores derive therefrom may be used in characterizing and/or sequencing a polymeric molecule or may be for use as molecular sensors.