Patents by Inventor Mattheos Koffas

Mattheos Koffas has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20240327781
    Abstract: Recombinant E. coli strains and synthetic protein sequence designs are leveraged for production of disordered polypeptides such as spidroins and elastin-like peptides (ELPs). These disordered polypeptides, the high-titer production of which has proven difficult, include repeating structural motifs from a small selection of amino acid residues, resulting in lack of well-defined tertiary and quaternary structure. The recombinant E. coli include expression vectors with genes encoding for the disordered polypeptide product. Expression of these genes is controlled by a promoter that downregulates and substantially inhibits basal expression in the recombinant bacteria. Further, the recombinant bacteria include mutations to one or more stress-response genes from wild-type E. coli, such as yggw, yedv, yedw, yedy, spec, speb, uspc, hcha, loip, mltc, envz, ompr, yhgf, or hupb. The recombinant E. coli enable production of high titers of disordered protein product while minimizing the toxic effects thereof on the host.
    Type: Application
    Filed: October 26, 2022
    Publication date: October 3, 2024
    Applicant: RENSSELAER POLYTECHNIC INSTITUTE
    Inventors: Alexander Allen CONNOR, Mattheos KOFFAS, Runye ZHA, Derek NELSON
  • Patent number: 11965197
    Abstract: Disclosed herein are novel microbial polycultures of two or more cell strains, capable of producing flavanones, flavonoids, and anthocyanidin-3-O-glucosides, and methods of use thereof. Also disclosed is a microbial cell capable of producing phenylpropanoic acids, and methods of use thereof.
    Type: Grant
    Filed: March 10, 2021
    Date of Patent: April 23, 2024
    Assignee: Rensselaer Polytechnic Institute
    Inventors: Mattheos Koffas, John Andrew Jones
  • Patent number: 11512328
    Abstract: A combination of an electrochemical device for delivering reducing equivalents to a cell, and engineered metabolic pathways within the cell capable of utilizing the electrochemically provided reducing equivalents is disclosed. Such a combination allows the production of commodity chemicals by fermentation to proceed with increased carbon efficiency.
    Type: Grant
    Filed: June 25, 2020
    Date of Patent: November 29, 2022
    Assignee: BIOCHEMINSIGHTS, INC.
    Inventors: David R. Dodds, William B. Armiger, Mattheos Koffas
  • Publication number: 20210198712
    Abstract: Disclosed herein are novel microbial polycultures of two or more cell strains, capable of producing flavanones, flavonoids, and anthocyanidin-3-O-glucosides, and methods of use thereof. Also disclosed is a microbial cell capable of producing phenylpropanoic acids, and methods of use thereof.
    Type: Application
    Filed: March 10, 2021
    Publication date: July 1, 2021
    Applicant: RENSSELAER POLYTECHNIC INSTITUTE
    Inventors: Mattheos KOFFAS, John Andrew JONES
  • Publication number: 20210171995
    Abstract: Provided herein are composition and process for using an electrochemical device for the reduction of the oxidized state of phosphorylated or non-phosphorylated nicotinamide adenine dinucleotide to the reduced state in which unwanted products of the electrochemical reduction are recovered as the oxidized state of the phosphorylated or non-phosphorylated nicotinamide adenine dinucleotide and returned to the electrochemical device for reduction.
    Type: Application
    Filed: February 22, 2021
    Publication date: June 10, 2021
    Inventors: Clifford S. Morrison, William B. Armiger, David R. Dodds, Mattheos Koffas
  • Patent number: 10954543
    Abstract: Disclosed herein are novel microbial polycultures of two or more cell strains, capable of producing flavanones, flavonoids, and anthocyanidin-3-O-glucosides, and methods of use thereof. Also disclosed is a microbial cell capable of producing phenylpropanoic acids, and methods of use thereof.
    Type: Grant
    Filed: November 2, 2016
    Date of Patent: March 23, 2021
    Assignee: Rensselaer Polytechnic Institute
    Inventors: Mattheos Koffas, John Andrew Jones
  • Patent number: 10927395
    Abstract: Provided herein a re composition and process for using an electrochemical device for the reduction of the oxidized state of phosphorylated or non-phosphorylated nicotinamide adenine dinucleotide to the reduced state in which unwanted products of the electrochemical reduction are recovered as the oxidized state of the phosphorylated or non-phosphorylated nicotinamide adenine dinucleotide and returned to the electrochemical device for reduction.
    Type: Grant
    Filed: March 14, 2017
    Date of Patent: February 23, 2021
    Assignee: Biocheminsights, Inc.
    Inventors: Clifford S. Morrison, William B. Armiger, David R. Dodds, Mattheos Koffas
  • Publication number: 20200325498
    Abstract: A combination of an electrochemical device for delivering reducing equivalents to a cell, and engineered metabolic pathways within the cell capable of utilizing the electrochemically provided reducing equivalents is disclosed. Such a combination allows the production of commodity chemicals by fermentation to proceed with increased carbon efficiency.
    Type: Application
    Filed: June 25, 2020
    Publication date: October 15, 2020
    Inventors: David R. Dodds, William B. Armiger, Mattheos Koffas
  • Patent number: 10696988
    Abstract: A combination of an electrochemical device for delivering reducing equivalents to a cell, and engineered metabolic pathways within the cell capable of utilizing the electrochemically provided reducing equivalents is disclosed. Such a combination allows the production of commodity chemicals by fermentation to proceed with increased carbon efficiency.
    Type: Grant
    Filed: February 23, 2016
    Date of Patent: June 30, 2020
    Assignee: Biocheminsights, Inc.
    Inventors: David R. Dodds, William B. Armiger, Mattheos Koffas
  • Publication number: 20190078127
    Abstract: Provided herein a re composition and process for using an electrochemica 1 device for the reduction of the oxidized state of phosphorylated or non-phosphorylated nicotinamide adenine dinucleotide to the reduced state in which unwanted products of the electrochemical reduction are recovered as the oxidized state of the phosphorylated or non-phosphorylated nicotinamide adenine dinucleotide and returned to the electrochemical device for reduction.
    Type: Application
    Filed: March 14, 2017
    Publication date: March 14, 2019
    Inventors: Clifford S. Morrison, William B. Armiger, David R. Dodds, Mattheos Koffas
  • Publication number: 20180037914
    Abstract: A combination of an electrochemical device for delivering reducing equivalents to a cell, and engineered metabolic pathways within the cell capable of utilizing the electrochemically provided reducing equivalents is disclosed. Such a combination allows the production of commodity chemicals by fermentation to proceed with increased carbon efficiency.
    Type: Application
    Filed: February 23, 2016
    Publication date: February 8, 2018
    Applicant: Biocheminsights, Inc.
    Inventors: David R. Dodds, William B. Armiger, Mattheos Koffas
  • Publication number: 20170121748
    Abstract: Disclosed herein are novel microbial polycultures of two or more cell strains, capable of producing flavanones, flavonoids, and anthocyanidin-3-O-glucosides, and methods of use thereof. Also disclosed is a microbial cell capable of producing phenylpropanoic acids, and methods of use thereof.
    Type: Application
    Filed: November 2, 2016
    Publication date: May 4, 2017
    Applicant: RENSSELAER POLYTECHNIC INSTITUTE
    Inventors: Mattheos KOFFAS, John Andrew JONES
  • Patent number: 9181539
    Abstract: The invention relates to the production of flavonoids and flavonoid precursors in cells through recombinant expression of tyrosine ammonia lyase (TAL), 4-coumarate:CoA ligase (4CL), chalcone synthase (CHS), and chalcone isomerase (CHI).
    Type: Grant
    Filed: May 5, 2011
    Date of Patent: November 10, 2015
    Assignees: Massachusetts Institute of Technology, The Research Foundation of State University of New York
    Inventors: Gregory Stephanopoulos, Christine Santos, Mattheos Koffas
  • Publication number: 20120034661
    Abstract: The invention relates to the production of flavonoids and flavonoid precursors in cells through recombinant expression of tyrosine ammonia lyase (TAL), 4-coumarate:CoA ligase (4CL), chalcone synthase (CHS), and chalcone isomerase (CHI).
    Type: Application
    Filed: May 5, 2011
    Publication date: February 9, 2012
    Applicants: The Research Foundation of State University of New York, Massachusetts Institute of Technology
    Inventors: Gregory Stephanopoulos, Christine Santos, Mattheos Koffas
  • Patent number: 7807422
    Abstract: Methods and compositions are provided for production of flavonoids in microbial hosts. The compositions comprises a set of genes which encode for enzymes involved in one or more steps in the biosynthetic pathway for the conversion of phenylpropanoids to various flavonoids. The method comprises the steps of introducing the set of genes into a heterologous host cell, allowing growth of the cells in a suitable medium such that the expression of the genes results in production of enzymes. When specific substrate(s) is/are provided to the transformed cell, the enzymes act on the substrate(s) to produce the desired flavonoids.
    Type: Grant
    Filed: March 3, 2008
    Date of Patent: October 5, 2010
    Assignee: The Research Foundation of State University of New York
    Inventors: Mattheos Koffas, Effendi Leonard, Yajun Yan, Joseph Chemler
  • Publication number: 20090239272
    Abstract: Methods and compositions are provided for production of flavonoids in microbial hosts. The compositions comprises a set of genes which encode for enzymes involved in one or more steps in the biosynthetic pathway for the conversion of phenylpropanoids to various flavonoids. The method comprises the steps of introducing the set of genes into a heterologous host cell, allowing growth of the cells in a suitable medium such that the expression of the genes results in production of enzymes. When specific substrate(s) is/are provided to the transformed cell, the enzymes act on the substrate(s) to produce the desired flavonoids.
    Type: Application
    Filed: March 3, 2008
    Publication date: September 24, 2009
    Inventors: Mattheos Koffas, Effendi Leonard, Yajun Yan, Joseph Chemler
  • Patent number: 7338791
    Abstract: Methods and compositions are provided for production of flavonoids in microbial hosts. The compositions comprises a set of genes which encode for enzymes involved in one or more steps in the biosymthetic pathway for the conversion of phenylpropanoids to various flavonoids. The method comprises the steps of introducing the set of genes into a heterologous host cell, allowing growth of the cells in a suitable medium such that the expression of the genes results in production of enzymes. When specific substrate(s) is/are provided to the transformed cell, the enzymes act on the substrate(s) to produce the desired flavonoids.
    Type: Grant
    Filed: July 11, 2005
    Date of Patent: March 4, 2008
    Assignee: The Research Foundation of State University of New York
    Inventors: Mattheos Koffas, Effendi Leonard, Yajun Yan, Joseph Chemler
  • Patent number: 7056717
    Abstract: Genes have been isolated from Methylomonas 16a sp. encoding the isoprenoid biosynthetic pathway. The genes and gene products are the first isolated from a Methylomonas strain that is capable of utilizing single carbon (C1) substrates as energy sources. The genes and gene products of the present invention may be used in a variety of ways for the production of isoprenoid compounds in a variety of organisms.
    Type: Grant
    Filed: November 3, 2003
    Date of Patent: June 6, 2006
    Assignee: E. I. du Pont de Nemours and Company
    Inventors: Qiong Cheng, Mattheos Koffas, Kelley C. Norton, James M. Odom, Stephen K. Picataggio, Andreas Schenzle, Jean-Francois Tomb, Pierre E. Rouviere
  • Publication number: 20060019334
    Abstract: Methods and compositions are provided for production of flavonoids in microbial hosts. The compositions comprises a set of genes which encode for enzymes involved in one or more steps in the biosymthetic pathway for the conversion of phenylpropanoids to various flavonoids. The method comprises the steps of introducing the set of genes into a heterologous host cell, allowing growth of the cells in a suitable medium such that the expression of the genes results in production of enzymes. When specific substrate(s) is/are provided to the transformed cell, the enzymes act on the substrate(s) to produce the desired flavonoids.
    Type: Application
    Filed: July 11, 2005
    Publication date: January 26, 2006
    Inventors: Mattheos Koffas, Effendi Leonard, Yajun Yan, Joseph Chemler
  • Patent number: 6969595
    Abstract: A method for the production of carotenoid compounds is disclosed. The method relies on the use of microorganisms which metabolize single carbon substrates for the production of carotenoid compounds in high yields.
    Type: Grant
    Filed: August 29, 2001
    Date of Patent: November 29, 2005
    Assignee: E. I. du Pont de Nemours and Company
    Inventors: Patricia C. Brzostowicz, Qiong Cheng, Deana DiCosimo, Mattheos Koffas, Edward S. Miller, James M. Odom, Stephen K. Picataggio, Pierre E. Rouviere