Abstract: A method for determining the effectiveness of the treatment of a fluid for the purpose of reducing or eliminating particles in the fluid. The method includes the steps of obtaining samples of the fluid before and after treatment, delivering the samples to a particle imaging system, obtaining image information of particles in the samples, including particle colors, and comparing the difference in particle color from the first sample to the second sample. A change in particle color detected is indicative of particle death.

Abstract: An imaging flow cytometry system and method which includes a flow chamber, fluorescence analysis and imaging optics, image capturing system, device to regulate fluid flow through the chamber, and backlighting generator. The flow cell is configured so as to enhance the fluorescence signal collection by the system with total internal reflections. The fluorescence collection optics are configured to enhance the collection of the fluorescence from the side of the flow cell and concentrate it on light detectors.

Abstract: A system for diluting a sample fluid sufficiently to enable the capture images of particles contained in the sample fluid. The system includes a fluid dilution system and a particle imaging system. The fluid dilution system includes a mixing conduit for combining a diluent and the sample solution. The mixing conduit is coupled to a flow chamber associated with imaging capturing devices including a camera. When the sample fluid is too opaque or viscous to enable capture of particle images of sufficient clarity, the fluid dilution system is activated to introduce diluent into the mixing conduit in sufficient volume to dilute the sample fluid. The diluted sample fluid is passed through the flow chamber and particle images captured. Information regarding captured images may be stored, analyzed and transferred from a remote location.

Abstract: An imaging flow cytometry system and method which includes a flow chamber, fluorescence analysis and imaging optics, image capturing system, device to regulate fluid flow through the chamber, and backlighting generator. The flow cell is configured so as to enhance the fluorescence signal collection by the system with total internal reflections. The fluorescence collection optics are configured to enhance the collection of the fluorescence from the side of the flow cell and concentrate it on light detectors.