Patents by Inventor Matthew B. Robers

Matthew B. Robers has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Oplophorus-derived luciferases, novel coelenterazine substrates, and methods of use
    Patent number: 11661623
    Abstract: An isolated polynucleotide encoding a modified luciferase polypeptide and substrates. The OgLuc variant polypeptide has at least 60% amino acid sequence identity to SEQ ID NO: 1 and at least one amino acid substitution at a position corresponding to an amino acid in SEQ ID NO: 1. The OgLuc variant polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the corresponding polypeptide of the wild-type Oplophorus luciferase.
    Type: Grant
    Filed: September 3, 2020
    Date of Patent: May 30, 2023
    Assignee: PROMEGA CORPORATION
    Inventors: Brock Binkowski, Lance P. Encell, Mary Hall, Matthew B. Robers, Michael R. Slater, Keith V. Wood, Monika G. Wood, Dieter Klaubert, Poncho Meisenheimer, James Unch, Michael P. Valley
  • OPLOPHORUS-DERIVED LUCIFERASES, NOVEL COELENTERAZINE SUBSTRATES, AND METHODS OF USE
    Publication number: 20220348983
    Abstract: An isolated polynucleotide encoding a modified luciferase polypeptide and substrates. The OgLuc variant polypeptide has at least 60% amino acid sequence identity to SEQ ID NO: 1 and at least one amino acid substitution at a position corresponding to an amino acid in SEQ ID NO: 1. The OgLuc variant polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the corresponding polypeptide of the wild-type Oplophorus luciferase.
    Type: Application
    Filed: May 27, 2021
    Publication date: November 3, 2022
    Inventors: Brock Binkowski, Lance P. Encell, Mary Hall, Matthew B. Robers, Michael R. Slater, Keith V. Wood, Monika G. Wood
  • TARGET ENGAGEMENT ASSAY FOR RAS PROTEINS
    Publication number: 20220064696
    Abstract: Provided herein are systems, methods, and compounds for identifying RAS binding compounds using a RAS binding agent, which comprises a RAS binding moiety and a functional element. In some embodiments, the RAS binding agent binds to one site on the RAS protein (e.g., KRAS, HRAS, or NRAS), and can be used to detect RAS binding agents that bind to the same site as well as other sites.
    Type: Application
    Filed: August 27, 2021
    Publication date: March 3, 2022
    Applicant: Promega Corporation
    Inventors: Matthew B. Robers, Joel R. Walker, James Vasta, Cesear Corona
  • OPLOPHORUS-DERIVED LUCIFERASES, NOVEL COELENTERAZINE SUBSTRATES, AND METHODS OF USE
    Publication number: 20200399676
    Abstract: An isolated polynucleotide encoding a modified luciferase polypeptide and substrates. The OgLuc variant polypeptide has at least 60% amino acid sequence identity to SEQ ID NO: 1 and at least one amino acid substitution at a position corresponding to an amino acid in SEQ ID NO: 1. The OgLuc variant polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the corresponding polypeptide of the wild-type Oplophorus luciferase.
    Type: Application
    Filed: September 3, 2020
    Publication date: December 24, 2020
    Inventors: Brock Binkowski, Lance P. Encell, Mary Hall, Matthew B. Robers, Michael R. Slater, Keith V. Wood, Monika G. Wood
  • Functionalized NANOLUC inhibitors
    Patent number: 10815247
    Abstract: Compounds that may inhibit Oplophorus-derived luciferases are disclosed as well as compositions and kits comprising the compounds, and methods of using the compounds.
    Type: Grant
    Filed: December 28, 2017
    Date of Patent: October 27, 2020
    Assignee: PROMEGA CORPORATION
    Inventors: Roderick G. Flemming, Mary Hall, Thomas Machleidt, Poncho Meisenheimer, Nidhi Nath, Matthew B. Robers, Joel R. Walker, Wenhui Zhou
  • Oplophorus-derived luciferases, novel coelenterazine substrates, and methods of use
    Patent number: 10774364
    Abstract: An isolated polynucleotide encoding a modified luciferase polypeptide and substrates. The OgLuc variant polypeptide has at least 60% amino acid sequence identity to SEQ ID NO: 1 and at least one amino acid substitution at a position corresponding to an amino acid in SEQ ID NO: 1. The OgLuc variant polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the corresponding polypeptide of the wild-type Oplophorus luciferase.
    Type: Grant
    Filed: April 9, 2018
    Date of Patent: September 15, 2020
    Assignee: Promega Corporation
    Inventors: Brock Binkowski, Lance P. Encell, Mary Hall, Matthew B. Robers, Michael R. Slater, Keith V. Wood, Monika G. Wood
  • THIENOPYRROLE COMPOUNDS AND USES THEREOF
    Publication number: 20200063110
    Abstract: Thienopyrrole compounds that may inhibit Oplophorus-derived luciferases are disclosed, as well as compositions and kits comprising the thienopyrrole compounds, and methods of using the thienopyrrole compounds.
    Type: Application
    Filed: November 5, 2019
    Publication date: February 27, 2020
    Inventors: Sarah Duellman, Matthew B. Robers, Joel R. Walker, Wenhui Zhou, Chad Zimprich
  • Thienopyrrole compounds and uses thereof
    Patent number: 10513694
    Abstract: Thienopyrrole compounds that may inhibit Oplophorus-derived luciferases are disclosed, as well as compositions and kits comprising the thienopyrrole compounds, and methods of using the thienopyrrole compounds.
    Type: Grant
    Filed: June 24, 2016
    Date of Patent: December 24, 2019
    Assignee: PROMEGA CORPORATION
    Inventors: Sarah Duellman, Matthew B. Robers, Joel R. Walker, Wenhui Zhou, Chad Zimprich
  • OPLOPHORUS-DERIVED LUCIFERASES, NOVEL COELENTERAZINE SUBSTRATES, AND METHODS OF USE
    Publication number: 20180327810
    Abstract: An isolated polynucleotide encoding a modified luciferase polypeptide and substrates. The OgLuc variant polypeptide has at least 60% amino acid sequence identity to SEQ ID NO: 1 and at least one amino acid substitution at a position corresponding to an amino acid in SEQ ID NO: 1. The OgLuc variant polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the corresponding polypeptide of the wild-type Oplophorus luciferase.
    Type: Application
    Filed: April 9, 2018
    Publication date: November 15, 2018
    Inventors: Brock Binkowski, Lance P. Encell, Mary Hall, Matthew B. Robers, Michael R. Slater, Keith V. Wood, Monika G. Wood
  • FUNCTIONALIZED NANOLUC INHIBITORS
    Publication number: 20180222916
    Abstract: Compounds that may inhibit Oplophorus-derived luciferases are disclosed as well as compositions and kits comprising the compounds, and methods of using the compounds.
    Type: Application
    Filed: December 28, 2017
    Publication date: August 9, 2018
    Inventors: Roderick G. Flemming, Mary Hall, Thomas Machleidt, Poncho Meisenheimer, Nidhi Nath, Matthew B. Robers, Joel R. Walker, Wenhui Zhou
  • Oplophorus-derived luciferases, novel coelenterazine substrates, and methods of use
    Patent number: 9951373
    Abstract: An isolated polynucleotide encoding a modified luciferase polypeptide and substrates. The OgLuc variant polypeptide has at least 60% amino acid sequence identity to SEQ ID NO: 1 and at least one amino acid substitution at a position corresponding to an amino acid in SEQ ID NO: 1. The OgLuc variant polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the corresponding polypeptide of the wild-type Oplophorus luciferase.
    Type: Grant
    Filed: January 21, 2014
    Date of Patent: April 24, 2018
    Assignee: Promega Corporation
    Inventors: Brock Binkowski, Lance P. Encell, Mary Hall, Matthew B. Robers, Michael R. Slater, Keith V. Wood, Monika G. Wood
  • Oplophorus-derived luciferases, novel coelenterazine substrates, and methods of use
    Patent number: 9840730
    Abstract: An isolated polynucleotide encoding a modified luciferase polypeptide and substrates. The OgLuc variant polypeptide has at least 60% amino acid sequence identity to SEQ ID NO: 1 and at least one amino acid substitution at a position corresponding to an amino acid in SEQ ID NO: 1. The OgLuc variant polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the corresponding polypeptide of the wild-type Oplophorus luciferase.
    Type: Grant
    Filed: January 21, 2014
    Date of Patent: December 12, 2017
    Assignee: PROMEGA CORPORATION
    Inventors: Brock Binkowski, Lance P. Encell, Mary Hall, Matthew B. Robers, Michael R. Slater, Keith V. Wood, Monika G. Wood
  • Compositions and methods for monitoring transmembrane trafficking
    Patent number: 9738921
    Abstract: Provided herein are compositions and methods for monitoring the movement of analytes and/or cellular components across biological membranes (e.g., cell surface internalization). In particular, reporter constructs are provided, the transmembrane movement of which (e.g., by endocytosis) is monitored by methods described herein.
    Type: Grant
    Filed: July 15, 2015
    Date of Patent: August 22, 2017
    Assignee: Promega Corporation
    Inventors: Brock F. Binkowski, Mei Cong, Matthew B. Robers
  • THIENOPYRROLE COMPOUNDS AND USES THEREOF
    Publication number: 20160376568
    Abstract: Thienopyrrole compounds that may inhibit Oplophorus-derived luciferases are disclosed, as well as compositions and kits comprising the thienopyrrole compounds, and methods of using the thienopyrrole compounds.
    Type: Application
    Filed: June 24, 2016
    Publication date: December 29, 2016
    Inventors: Sarah Duellman, Matthew B. Robers, Joel R. Walker, Wenhui Zhou, Chad Zimprich
  • COMPOSITIONS AND METHODS FOR MONITORING TRANSMEMBRANE TRAFFICKING
    Publication number: 20150322482
    Abstract: Provided herein are compositions and methods for monitoring the movement of analytes and/or cellular components across biological membranes (e.g., cell surface internalization). In particular, reporter constructs are provided, the transmembrane movement of which (e.g., by endocytosis) is monitored by methods described herein.
    Type: Application
    Filed: July 15, 2015
    Publication date: November 12, 2015
    Inventors: Brock F. Binkowski, Mei Cong, Matthew B. Robers
  • Compositions and methods for monitoring transmembrane trafficking
    Patent number: 9096889
    Abstract: Provided herein are compositions and methods for monitoring the movement of analytes and/or cellular components across biological membranes (e.g., cell surface internalization). In particular, reporter constructs are provided, the transmembrane movement of which (e.g., by endocytosis) is monitored by methods described herein.
    Type: Grant
    Filed: November 21, 2012
    Date of Patent: August 4, 2015
    Assignee: PROMEGA CORPORATION
    Inventors: Brock F. Binkowski, Mei Cong, Matthew B. Robers
  • OPLOPHORUS-DERIVED LUCIFERASES, NOVEL COELENTERAZINE SUBSTRATES, AND METHODS OF USE
    Publication number: 20140227759
    Abstract: An isolated polynucleotide encoding a modified luciferase polypeptide and substrates. The OgLuc variant polypeptide has at least 60% amino acid sequence identity to SEQ ID NO: 1 and at least one amino acid substitution at a position corresponding to an amino acid in SEQ ID NO: 1. The OgLuc variant polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the corresponding polypeptide of the wild-type Oplophorus luciferase.
    Type: Application
    Filed: January 21, 2014
    Publication date: August 14, 2014
    Applicant: PROMEGA CORPORATION
    Inventors: Brock Binkowski, Lance P. Encell, Mary Hall, Matthew B. Robers, Michael R. Slater, Keith V. Wood, Monika G. Wood
  • OPLOPHORUS-DERIVED LUCIFERASES, NOVEL COELENTERAZINE SUBSTRATES, AND METHODS OF USE
    Publication number: 20140223590
    Abstract: An isolated polynucleotide encoding a modified luciferase polypeptide and substrates. The OgLuc variant polypeptide has at least 60% amino acid sequence identity to SEQ ID NO: 1 and at least one amino acid substitution at a position corresponding to an amino acid in SEQ ID NO: 1. The OgLuc variant polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the corresponding polypeptide of the wild-type Oplophorus luciferase.
    Type: Application
    Filed: January 21, 2014
    Publication date: August 7, 2014
    Applicant: Promego Corporation
    Inventors: Brock Binkowski, Lance P. Encell, Mary Hall, Matthew B. Robers, Michael R. Slater, Keith V. Wood, Monika G. Wood
  • Oplophorus-derived luciferases, novel coelenterazine substrates, and methods of use
    Patent number: 8669103
    Abstract: An isolated polynucleotide encoding a modified luciferase polypeptide and substrates. The OgLuc variant polypeptide has at least 60% amino acid sequence identity to SEQ ID NO: 1 and at least one amino acid substitution at a position corresponding to an amino acid in SEQ ID NO: 1. The OgLuc variant polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the corresponding polypeptide of the wild-type Oplophorus luciferase.
    Type: Grant
    Filed: November 2, 2011
    Date of Patent: March 11, 2014
    Assignee: Promega Corporation
    Inventors: Brock Binkowski, Lance P. Encell, Mary Hall, Matthew B. Robers, Michael R. Slater, Keith V. Wood, Monika G. Wood
  • COMPOSITIONS AND METHODS FOR MONITORING TRANSMEMBRANE TRAFFICKING
    Publication number: 20130196357
    Abstract: Provided herein are compositions and methods for monitoring the movement of analytes and/or cellular components across biological membranes (e.g., cell surface internalization). In particular, reporter constructs are provided, the transmembrane movement of which (e.g., by endocytosis) is monitored by methods described herein.
    Type: Application
    Filed: November 21, 2012
    Publication date: August 1, 2013
    Inventors: Brock F. Binkowski, Mei Cong, Matthew B. Robers