Patents by Inventor Matthew Bahr
Matthew Bahr has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11953420Abstract: Light detection systems for measuring light (e.g., in a flow stream) are described. Light detection systems according to embodiments include a light scatter detector, a brightfield photodetector and an optical adjustment component configured to convey light to the light scatter detector and to the brightfield photodetector. Systems and methods for measuring light emitted by a sample (e.g., in a flow stream) and kits having a light scatter detector, a brightfield photodetector and a beam splitter component are also provided.Type: GrantFiled: March 15, 2021Date of Patent: April 9, 2024Assignee: BECTON, DICKINSON AND COMPANYInventors: Jizuo Zou, Matthew Bahr, Eric D. Diebold
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Patent number: 11940372Abstract: Aspects of the present disclosure include a method for sorting cells of a sample based on an image of a cell in a flow stream. Methods according to certain embodiments include detecting light from a sample having cells in a flow stream, generating an image mask of a cell from the sample and sorting the cell based on the generated image mask. Systems having a processor with memory operably coupled to the processor having instructions stored thereon, which when executed by the processor, cause the processor to generate an image mask of a cell in a sample in a flow stream and to sort the cell based on the generated image mask are also described. Integrated circuit devices (e.g., field programmable gate arrays) having programming for generating an image mask and for determining one or more features of the cell are also provided.Type: GrantFiled: September 28, 2022Date of Patent: March 26, 2024Assignee: BECTON, DICKINSON AND COMPANYInventors: Jonathan Lin, Matthew Bahr, Keegan Owsley
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Publication number: 20240019354Abstract: Aspects of the present disclosure include methods for characterizing particles of a sample in a flow stream. Methods according to certain embodiments include generating frequency-encoded fluorescence data from a particle of a sample in a flow stream; and calculating phase-corrected spatial data of the particle by performing a transform of the frequency-encoded fluorescence data with a phase correction component. In certain embodiments, methods include generating an image of the particle in the flow stream based on the phase-corrected spatial data. Systems having a processor with memory operably coupled to the processor having instructions stored thereon, which when executed by the processor, cause the processor to calculate phase-corrected spatial data from frequency-encoded fluorescence data of a particle a flow stream are also described. Integrated circuit devices (e.g., field programmable gate arrays) having programming for practicing the subject methods are also provided.Type: ApplicationFiled: September 27, 2023Publication date: January 18, 2024Inventors: Jonathan Lin, Keegan Owsley, Matthew Bahr
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Patent number: 11808690Abstract: Aspects of the present disclosure include methods for characterizing particles of a sample in a flow stream. Methods according to certain embodiments include generating frequency-encoded fluorescence data from a particle of a sample in a flow stream; and calculating phase-corrected spatial data of the particle by performing a transform of the frequency-encoded fluorescence data with a phase correction component. In certain embodiments, methods include generating an image of the particle in the flow stream based on the phase-corrected spatial data. Systems having a processor with memory operably coupled to the processor having instructions stored thereon, which when executed by the processor, cause the processor to calculate phase-corrected spatial data from frequency-encoded fluorescence data of a particle a flow stream are also described. Integrated circuit devices (e.g., field programmable gate arrays) having programming for practicing the subject methods are also provided.Type: GrantFiled: January 11, 2023Date of Patent: November 7, 2023Assignee: BECTON, DICKINSON AND COMPANYInventors: Jonathan Lin, Keegan Owsley, Matthew Bahr
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Publication number: 20230349812Abstract: In one aspect, a system for performing flow cytometry is disclosed, which comprises a laser for generating laser radiation for illuminating a sample, at least one detector for detecting at least a portion of a radiation emanating from the sample in response to said illumination so as to generate a temporal signal corresponding to said detected radiation, and an analysis module for receiving said temporal signal and performing a statistical analysis of said signal based on a forward model to reconstruct an image of said sample.Type: ApplicationFiled: June 26, 2023Publication date: November 2, 2023Inventors: Eric Diebold, Keegan Owsley, Jonathan Lin, Matthew Bahr
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Publication number: 20230296494Abstract: Aspects of the present disclosure include methods for producing an output laser beam having two or more angularly deflected laser beams (e.g., for irradiating a sample in a flow stream) with a predetermined intensity profile. Systems for practicing the subject methods having a laser, an acousto-optic device, a radiofrequency generator and a controller for adjusting the amplitude of the radiofrequency drive signals to produce an output laser beam of angularly deflected laser beams with a predetermined intensity profile are also described.Type: ApplicationFiled: May 19, 2023Publication date: September 21, 2023Inventors: Eric D. Diebold, Keegan Owsley, Matthew Bahr
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Publication number: 20230228668Abstract: Disclosed herein include embodiments of a system, a device, and a method for sorting a plurality cells of a sample. A plurality of raw images comprising pixels of complex values in a frequency space can be generated from a plurality of channels of fluorescence intensity data of fluorescence emissions of fluorophores, the fluorescence emissions being elicited by fluorescence imaging using radiofrequency-multiplexed excitation in a temporal space. Spectral unmixing can be performed on the raw images prior to a sorting decision being made.Type: ApplicationFiled: February 24, 2023Publication date: July 20, 2023Inventors: Matthew Bahr, Keegan Owsley
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Patent number: 11698334Abstract: Aspects of the present disclosure include methods for producing an output laser beam having two or more angularly deflected laser beams (e.g., for irradiating a sample in a flow stream) with a predetermined intensity profile. Systems for practicing the subject methods having a laser, an acousto-optic device, a radiofrequency generator and a controller for adjusting the amplitude of the radiofrequency drive signals to produce an output laser beam of angularly deflected laser beams with a predetermined intensity profile are also described.Type: GrantFiled: August 26, 2020Date of Patent: July 11, 2023Assignee: BECTON, DICKINSON AND COMPANYInventors: Eric D. Diebold, Keegan Owsley, Matthew Bahr
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Publication number: 20230144214Abstract: Aspects of the present disclosure include methods for characterizing particles of a sample in a flow stream. Methods according to certain embodiments include generating frequency-encoded fluorescence data from a particle of a sample in a flow stream; and calculating phase-corrected spatial data of the particle by performing a transform of the frequency-encoded fluorescence data with a phase correction component. In certain embodiments, methods include generating an image of the particle in the flow stream based on the phase-corrected spatial data. Systems having a processor with memory operably coupled to the processor having instructions stored thereon, which when executed by the processor, cause the processor to calculate phase-corrected spatial data from frequency-encoded fluorescence data of a particle a flow stream are also described. Integrated circuit devices (e.g., field programmable gate arrays) having programming for practicing the subject methods are also provided.Type: ApplicationFiled: January 11, 2023Publication date: May 11, 2023Inventors: Jonathan Lin, Keegan Owsley, Matthew Bahr
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Patent number: 11625829Abstract: Disclosed herein include embodiments of a system, a device, and a method for sorting a plurality cells of a sample. A plurality of raw images comprising pixels of complex values in a frequency space can be generated from a plurality of channels of fluorescence intensity data of fluorescence emissions of fluorophores, the fluorescence emissions being elicited by fluorescence imaging using radiofrequency-multiplexed excitation in a temporal space. Spectral unmixing can be performed on the raw images prior to a sorting decision being made.Type: GrantFiled: April 6, 2022Date of Patent: April 11, 2023Assignee: Becton, Dickinson and CompanyInventors: Matthew Bahr, Keegan Owsley
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Publication number: 20230057188Abstract: Aspects of the present disclosure include a method for sorting cells of a sample based on an image of a cell in a flow stream. Methods according to certain embodiments include detecting light from a sample having cells in a flow stream, generating an image mask of a cell from the sample and sorting the cell based on the generated image mask. Systems having a processor with memory operably coupled to the processor having instructions stored thereon, which when executed by the processor, cause the processor to generate an image mask of a cell in a sample in a flow stream and to sort the cell based on the generated image mask are also described. Integrated circuit devices (e.g., field programmable gate arrays) having programming for generating an image mask and for determining one or more features of the cell are also provided.Type: ApplicationFiled: September 28, 2022Publication date: February 23, 2023Inventors: Jonathan Lin, Matthew Bahr, Keegan Owsley
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Publication number: 20230053275Abstract: Aspects of the present disclosure include methods for modulating an intensity profile of a laser beam. Methods according to certain embodiments include irradiating an acousto-optic device with a laser to generate an output laser beam having a plurality of angularly deflected laser beams, capturing an image of the output laser beam, determining an intensity profile of the output laser beam along a horizontal axis from the captured image and adjusting one or more parameters of a waveform inputted into the acousto-optic device in response to the determined intensity profile to generate an output laser beam having a modulated intensity profile. Systems having a laser, an acousto-optic device, an imaging sensor and a waveform generator as well as non-transitory computer readable storage medium with instructions for practicing the subject methods are also described.Type: ApplicationFiled: October 27, 2022Publication date: February 16, 2023Inventors: Matthew Bahr, Keegan Owsley, Jizuo Zou
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Patent number: 11579071Abstract: Aspects of the present disclosure include methods for characterizing particles of a sample in a flow stream. Methods according to certain embodiments include generating frequency-encoded fluorescence data from a particle of a sample in a flow stream; and calculating phase-corrected spatial data of the particle by performing a transform of the frequency-encoded fluorescence data with a phase correction component. In certain embodiments, methods include generating an image of the particle in the flow stream based on the phase-corrected spatial data. Systems having a processor with memory operably coupled to the processor having instructions stored thereon, which when executed by the processor, cause the processor to calculate phase-corrected spatial data from frequency-encoded fluorescence data of a particle a flow stream are also described. Integrated circuit devices (e.g., field programmable gate arrays) having programming for practicing the subject methods are also provided.Type: GrantFiled: May 29, 2020Date of Patent: February 14, 2023Assignee: BECTON, DICKINSON AND COMPANYInventors: Jonathan Lin, Keegan Owsley, Matthew Bahr
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Publication number: 20230022846Abstract: Aspects of the present disclosure include methods for determining a photodetector gain correction factor for application to flow cytometer data. Methods according to certain embodiments include detecting light with a light detection system across a horizontal axis of a flow stream, generating data signals in a photodetector channel (e.g., an imaging photodetector channel) of the light detection system at a plurality of positions across the flow stream and calculating a detector gain correction factor for each position across the flow stream in response to the generated data signals. Methods also include applying a detector gain correction factor to data signals from a photodetector channel (e.g., non-imaging photodetector channels) to generate adjusted signal intensities. Systems (e.g., particle analyzers) having a light source and a light detection system that includes a photodetector (e.g., an imaging photodetector) for practicing the subject methods are also described.Type: ApplicationFiled: June 24, 2022Publication date: January 26, 2023Inventors: Jizuo Zou, Keegan Owsley, Matthew Bahr
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Publication number: 20220391929Abstract: Systems and methods to provide a question series are disclosed. Exemplary implementations may: receive and store in electronic storage questions, parameters, criteria to the parameters, and events from a user; generate, based on the parameters and the questions, a question series for presentation to individual ones of the consumers; effectuate presentation of a set of the questions of the question series via individual client computing platforms associated with the consumers; obtain responses to the set of the questions from the consumers; obtain indications of the occurrence of the events related to the individual consumers; adjust the ensuing questions for presentation of the question series based on the occurrence of the events and/or the responses; and effectuate presentation of a subsequent set of the questions of the question series via individual client computing platforms of the consumers based on the adjustment to the ensuing questions.Type: ApplicationFiled: June 4, 2021Publication date: December 8, 2022Inventors: Matthew Bahr, Curt Hasselschwert
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Patent number: 11513058Abstract: Aspects of the present disclosure include methods for modulating an intensity profile of a laser beam. Methods according to certain embodiments include irradiating an acousto-optic device with a laser to generate an output laser beam having a plurality of angularly deflected laser beams, capturing an image of the output laser beam, determining an intensity profile of the output laser beam along a horizontal axis from the captured image and adjusting one or more parameters of a waveform inputted into the acousto-optic device in response to the determined intensity profile to generate an output laser beam having a modulated intensity profile. Systems having a laser, an acousto-optic device, an imaging sensor and a waveform generator as well as non-transitory computer readable storage medium with instructions for practicing the subject methods are also described.Type: GrantFiled: May 6, 2021Date of Patent: November 29, 2022Assignee: BECTON, DICKINSON AND COMPANYInventors: Matthew Bahr, Keegan Owsley, Jizuo Zou
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Patent number: 11493427Abstract: Aspects of the present disclosure include a method for sorting cells of a sample based on an image of a cell in a flow stream. Methods according to certain embodiments include detecting light from a sample having cells in a flow stream, generating an image mask of a cell from the sample and sorting the cell based on the generated image mask. Systems having a processor with memory operably coupled to the processor having instructions stored thereon, which when executed by the processor, cause the processor to generate an image mask of a cell in a sample in a flow stream and to sort the cell based on the generated image mask are also described. Integrated circuit devices (e.g., field programmable gate arrays) having programming for generating an image mask and for determining one or more features of the cell are also provided.Type: GrantFiled: March 12, 2020Date of Patent: November 8, 2022Assignee: BECTON, DICKINSON AND COMPANYInventors: Jonathan Lin, Matthew Bahr, Keegan Owsley
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Publication number: 20220236164Abstract: Aspects of the present disclosure include methods for determining drop delay of a particle in a flow stream (e.g., in a particle analyzer). Methods according to certain embodiments include irradiating the particle in the flow stream with two or more spatially separated lasers, detecting light from the particle in a first photodetector channel and a second photodetector channel calculating a velocity of the particle in the flow stream based on the detected light in the first photodetector channel and the second photodetector channel and determining the drop delay of the particle based on the calculated velocity. Systems (e.g., particle analyzers) having a light source with two or more spatially separated lasers and a light detection system for practicing the subject methods are also described. Integrated circuits and non-transitory computer readable storage medium having instructions stored thereon for determining drop delay according to the subject methods are also provided.Type: ApplicationFiled: December 8, 2021Publication date: July 28, 2022Inventors: Matthew Bahr, Christopher Ghazi, Keegan Owsley
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Publication number: 20220230317Abstract: Disclosed herein include embodiments of a system, a device, and a method for sorting a plurality cells of a sample. A plurality of raw images comprising pixels of complex values in a frequency space can be generated from a plurality of channels of fluorescence intensity data of fluorescence emissions of fluorophores, the fluorescence emissions being elicited by fluorescence imaging using radiofrequency-multiplexed excitation in a temporal space. Spectral unmixing can be performed on the raw images prior to a sorting decision being made.Type: ApplicationFiled: April 6, 2022Publication date: July 21, 2022Inventors: Matthew Bahr, Keegan Owsley
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Publication number: 20220187182Abstract: Aspects of the present disclosure include methods for characterizing particles of a sample in a flow stream. Methods according to certain embodiments include detecting light from a sample having cells in a flow stream, generating an image of an object in the flow stream in an interrogation region and determining whether the object in the flow stream is an aggregate based on the generated image. Systems having a processor with memory operably coupled to the processor having instructions stored thereon, which when executed by the processor, cause the processor to generate an image of an object in a flow stream and to determine whether the object is an aggregate are also described. Integrated circuit devices (e.g., field programmable gate arrays) having programming for practicing the subject methods are also provided.Type: ApplicationFiled: January 28, 2022Publication date: June 16, 2022Inventors: Matthew Bahr, Eric D. Diebold, Jonathan Lin, Keegan Owsley