Abstract: A method used to detect endotoxin uses a system (10) having bidirectional pump (20), multiport valve (30), fluid section valve (170), fluid holding member (50), solenoid valve (60), air source (63) and detector (100). Sample, LAL reagent and chromogenic substrate fluid segments are introduced into the holding member (50) between air segments, the reagents are then mixed and introduced into the detector (100) for measurement The system can be washed with an alkaline solution and followed by cthanol, or washed with tricthylaminc followed by water.

Abstract: An apparatus and method for on-line testing for the presence of an endotoxin within a fluid sample from a fluid line. The apparatus is positioned in fluid communication with a fluid line to perform the on-line fluid testing for the presence of at least one endotoxin. The apparatus can include a housing and a fluid sampling system positioned in fluid communication with the fluid line. The fluid sampling system can comprise a valve for controlling the fluid flow from the fluid line into the fluid sampling system. A fluid flow well is positioned within the housing and in fluid communication with the fluid sampling system. A removable assembly can also be secured within the housing. The removable assembly comprises a plurality of wells for receiving used and unused fluid carrying members that can receive samples from the fluid flow well, a plurality of fluid sample receiving wells, and a plurality of vessel retention positions comprising recesses for securely receiving portions of respective fluid vessels.