Abstract: Method and apparatus for a pest control device mounting system configured for mounting pest control devices in a building and using them to control pests. The pest control device mounting system including a mounting bracket, which may have a circumferential portion defining a portal. In some examples, the mounting bracket includes a flange portion suitable for attaching to the perimeter of a cavity in a building surface. The pest control device mounting system including a plug configured to be removably attached to the mounting bracket and to substantially close the portal when attached to the mounting bracket, wherein the plug is configured to accommodate a pest control device.

Abstract: Method and apparatus for a pest control device mounting system configured for mounting pest control devices in a building and using them to control pests. The pest control device mounting system including a mounting bracket, which may have a circumferential portion defining a portal. In some examples, the mounting bracket includes a flange portion suitable for attaching to the perimeter of a cavity in a building surface. The pest control device mounting system including a plug configured to be removably attached to the mounting bracket and to substantially close the portal when attached to the mounting bracket, wherein the plug is configured to accommodate a pest control device.

Abstract: There is provided a method of computing resource allocation. The method comprises allocating a first bounded amount of computing resources forming a first set of computing resources; exclusively assigning the first set of computing resources to a first process of a computer program; receiving a request from the first process for additional computing resources; in response to the request from the first process, allocating a second bounded amount of computing resources forming a second set of computing resources; and spawning a second process from the first process and exclusively assigning the second set of computing resources to the second process; wherein this method may be repeated indefinitely by the first process, second process, or any other process created according to this method. By following this method, a process does not control the amount of computing resources allocated to that process (i.e., itself), but instead controls the amount of computing resources allocated to its child processes.

Abstract: Described herein are methods of modifying a target gene in a patient or in a patient-derived cell, wherein the patient has an autosomal recessive disorder with compound heterozygous mutations, the methods including delivering a first modified guide RNA, a second modified guide RNA, a Cas9 polypeptide, a biotin-binding molecule, a first biotinylated donor polynucleotide, and a second biotinylated donor polynucleotide. The first modified guide RNA and the first biotinylated donor polynucleotide correct a first diseased allele, and the second modified guide RNA and the second biotinylated donor polynucleotide correct a second diseased allele.

Abstract: Described herein are guide RNAs and modified guide RNAs suitable for biallelic correction of Pompe disease. Also included are methods of modifying a target gene in a patient or in a patient-derived cell, wherein the patient has an autosomal recessive disorder with compound heterozygous mutations, the methods including delivering a first modified guide RNA, a second modified guide RNA, a Cas9 polypeptide, a biotin-binding molecule, a first biotinylated donor polynucleotide, and a second biotinylated donor polynucleotide. The first modified guide RNA and the first biotinylated donor polynucleotide correct a first diseased allele, and the second modified guide RNA and the second biotinylated donor polynucleotide correct a second diseased allele.

Abstract: Method and apparatus for a pest control device mounting system configured for mounting pest control devices in a building and using them to control pests. The pest control device mounting system including a mounting bracket, which may have a circumferential portion defining a portal. In some examples, the mounting bracket includes a flange portion suitable for attaching to the perimeter of a cavity in a building surface. The pest control device mounting system including a plug configured to be removably attached to the mounting bracket and to substantially close the portal when attached to the mounting bracket, wherein the plug is configured to accommodate a pest control device.

Abstract: Method and apparatus for a pest control device mounting system configured for mounting pest control devices in a building and using them to control pests. The pest control device mounting system including a mounting bracket, which may have a circumferential portion defining a portal. In some examples, the mounting bracket includes a flange portion suitable for attaching to the perimeter of a cavity in a building surface. The pest control device mounting system including a plug configured to be removably attached to the mounting bracket and to substantially close the portal when attached to the mounting bracket, wherein the plug is configured to accommodate a pest control device.

Abstract: There is provided a method of computing resource allocation. The method comprises allocating a first bounded amount of computing resources forming a first set of computing resources; exclusively assigning the first set of computing resources to a first process of a computer program; receiving a request from the first process for additional computing resources; in response to the request from the first process, allocating a second bounded amount of computing resources forming a second set of computing resources; and spawning a second process from the first process and exclusively assigning the second set of computing resources to the second process; wherein this method may be repeated indefinitely by the first process, second process, or any other process created according to this method. By following this method, a process does not control the amount of computing resources allocated to that process (i.e., itself), but instead controls the amount of computing resources allocated to its child processes.

Abstract: Described herein are modified guide RNAs such as a single guide RNA including, from 5? to 3?, a single-stranded protospacer sequence, a first complementary strand of a binding region for the Cas9 polypeptide, an aptamer that binds a biotin-binding molecule, and a second complementary strand of the binding region for the Cas9 polypeptide. Also described is an RNP complex including the modified guide RNA and a Cas9 polypeptide or active fragment thereof. Also included are methods of modifying target genes in cells using the modified guide RNAs.

Abstract: Described herein are modified guide RNAs such as a single guide RNA including, from 5? to 3?, a single-stranded protospacer sequence, a first complementary strand of a binding region for the Cas9 polypeptide, an aptamer that binds a biotin-binding molecule, and a second complementary strand of the binding region for the Cas9 polypeptide. Also described is an RNP complex including the modified guide RNA and a Cas9 polypeptide or active fragment thereof. Also included are methods of modifying target genes in cells using the modified guide RNAs.

Abstract: Described herein are guide RNAs and modified guide RNAs suitable for biallelic correction of Pompe disease. Also included are methods of modifying a target gene in a patient or in a patient-derived cell, wherein the patient has an autosomal recessive disorder with compound heterozygous mutations, the methods including delivering a first modified guide RNA, a second modified guide RNA, a Cas9 polypeptide, a biotin-binding molecule, a first biotinylated donor polynucleotide, and a second biotinylated donor polynucleotide. The first modified guide RNA and the first biotinylated donor polynucleotide correct a first diseased allele, and the second modified guide RNA and the second biotinylated donor polynucleotide correct a second diseased allele.

Abstract: Method and apparatus for a pest control device mounting system configured for mounting pest control devices in a building and using them to control pests. The pest control device mounting system including a mounting bracket, which may have a circumferential portion defining a portal. In some examples, the mounting bracket includes a flange portion suitable for attaching to the perimeter of a cavity in a building surface. The pest control device mounting system including a plug configured to be removably attached to the mounting bracket and to substantially close the portal when attached to the mounting bracket, wherein the plug is configured to accommodate a pest control device.

Abstract: Described herein are modified guide RNAs such as a single guide RNA including, from 5? to 3?, a single-stranded protospacer sequence, a first complementary strand of a binding region for the Cas9 polypeptide, an aptamer that binds a biotin-binding molecule, and a second complementary strand of the binding region for the Cas9 polypeptide. Also described is an RNP complex including the modified guide RNA and a Cas9 polypeptide or active fragment thereof. Also included are methods of modifying target genes in cells using the modified guide RNAs.

Abstract: Disclosed are methods for cell transfection and regulating cellular behavior. More particularly, the present disclosure relates to methods of non-viral cell transfection and regulating cellular behavior using mineral coatings that allow for the enhanced transfection of cells with reduced cytotoxicity. The mineral coatings bind biomaterials and provide a source of calcium and phosphate ions to enhance transfection. The present disclosure also provides a high throughput platform for screening non-viral transfection of cells. The methods of the present disclosure also provide an advantageous biomaterial delivery platform because the mineral coatings may be deposited on various medical device materials after being specifically developed using the high throughput screening platform.

Abstract: Real time approval control systems and methods are provided. In at least some embodiments, a presence-based network is utilized to provide a parent or other adult who is in a position to approve or deny a request, more timely access to information to facilitate the decision-making process.

Abstract: Interactive robot (BOT) creation is described. A BOT is an automated computer program implemented on a presence-based network, such as an instant messaging (IM) network or voice over IP (VoIP) network. The BOT is implemented to interact with a user via a direct two-way communication to gather data. The data that is gathered is then used by the BOT to automatically create a new, user-defined BOT implemented on the same presence-based network.

Abstract: An interactive agent, or bot, is disclosed which is capable of formatting information for optimal presentation depending at least in part on the functionality of the endpoint device receiving the information. The bot may operate as part of an IM application interface which provides protocols for network communications between a user endpoint device and the bot.

Abstract: Advertisements are incorporated into alerts generated for a user. A server-side alert delivery system can be used by a partner server, client device, or other server to deliver an alert to a user about some event the user has requested. A relevant advertisement is selected based upon the content of the alert and other data. In some cases, the time of day of the alert and/or the location to which the alert is delivered may be considered in selecting the advertisement. The alert may be provided to a user through different services and devices, including an instant messaging service, mail service, or through a mobile device.