Abstract: Devices for sorting cells in a fluid sample are provided. The device includes a microfluidic conduit configured to carry a flow of a fluid sample. The conduit includes a longitudinal divider extending from an interior surface of the conduit towards an opposing interior surface of the conduit, where the longitudinal divider defines a first longitudinal fluid flow path in fluid communication with a second longitudinal fluid flow path in the conduit. Also provided are methods of using the devices as well as systems and kits that include the devices. The devices, systems and methods find use in a variety of different applications, including diagnostic assays.