Abstract: Various systems and methods are provided for surgical and interventional planning, support, post-operative follow-up, and functional recovery tracking. In general, a patient can be tracked throughout medical treatment including through initial onset of symptoms, diagnosis, non-surgical treatment, surgical treatment, and recovery from the surgical treatment. In one embodiment, a patient and one or more medical professionals involved with treating the patient can electronically access a comprehensive treatment planning, support, and review system. The system can provide recommendations regarding diagnosis, non-surgical treatment, surgical treatment, and recovery from the surgical treatment based on data gathered from the patient and the medical professional(s).

Abstract: The present disclosure provides variant OmpG polypeptides, compositions comprising the OmpG variant polypeptides, and methods for using the variant OmpG polypeptides as nanopores for determining the sequence of single stranded nucleic acids. The variant OmpG nanopores reduce the ionic current noise versus the parental OmpG polypeptide from which they are derived and thereby enable sequencing of polynucleotides with single nucleotide resolution. The reduced ionic current noise also provides for the use of these OmpG nanopore variants in other single molecule sensing applications, e.g., protein sequencing.

Abstract: Described herein are variants of alpha-hemolysin having at least one amino acid substitution at H35G, E111N, M113A, and/or K147N in the mature, wild-type alpha-hemolysin amino acid sequence. In certain examples, the variant may have a substitution at E111S, M113S, T145S, K147S, or L135I in the mature alpha-hemolysin amino acid sequence. The ?-hemolysin variants may also include a substitution at H144A and/or a series of glycine residues spanning residues 127 to 131 of the mature, wild-type alpha hemolysin. Also provided are nanopore assemblies including the alpha-hemolysin variants, the assembly having an increased nanopore lifetime. Further, provided are variants that, in addition to providing increased lifetime, provide a decreased time-to-thread. Hence, the variants provided herein both increase nanopore lifetime and improve efficiency and accuracy of DNA sequencing reactions using nanopores comprising the variants.

Abstract: The present disclosure provides variant OmpG polypeptides, compositions comprising the OmpG variant polypeptides, and methods for using the variant OmpG polypeptides as nanopores for determining the sequence of single stranded nucleic acids. The variant OmpG nanopores reduce the ionic current noise versus the parental OmpG polypeptide from which they are derived and thereby enable sequencing of polynucleotides with single nucleotide resolution. The reduced ionic current noise also provides for the use of these OmpG nanopore variants in other single molecule sensing applications, e.g., protein sequencing.

Abstract: Described herein are variants of alpha-hemolysin having at least one amino acid substitution at H35G, E111N, M113A, and/or K147N in the mature, wild-type alpha-hemolysin amino acid sequence. In certain examples, the variant may have a substitution at E111S, M113S, T145S, K147S, or L135I in the mature alpha-hemolysin amino acid sequence. The ?-hemolysin variants may also include a substitution at H144A and/or a series of glycine residues spanning residues 127 to 131 of the mature, wild-type alpha hemolysin. Also provided are nanopore assemblies including the alpha-hemolysin variants, the assembly having an increased nanopore lifetime. Further, provided are variants that, in addition to providing increased lifetime, provide a decreased time-to-thread. Hence, the variants provided herein both increase nanopore lifetime and improve efficiency and accuracy of DNA sequencing reactions using nanopores comprising the variants.

Abstract: A consumable device used in a nanopore based sequencing system can include a nanopore chip, a flow cell with one or more flow channels, and a flow cell cover. A fluidic interface can be used to deliver fluid to the flow cell. The fluid interface can include a flow cell boss and the flow cell cover can include a receptacle for receiving the flow cell boss. A dispense tip can be used to introduce fluid into the flow cell through the flow cell boss.

Abstract: Described herein are variants of alpha-hemolysin having at least one amino acid substitution at H35G, E111N, M113A, and/or K147N in the mature, wild-type alpha-hemolysin amino acid sequence. In certain examples, the variant may have a substitution at E111S, M113S, T145S, K147S, or L1351 in the mature alpha-hemolysin amino acid sequence. The ?-hemolysin variants may also include a substitution at H144A and/or a series of glycine residues spanning residues 127 to 131 of the mature, wild-type alpha hemolysin. Also provided are nanopore assemblies including the alpha-hemolysin variants, the assembly having an increased nanopore lifetime. Further, provided are variants that, in addition to providing increased lifetime, provide a decreased time-to-thread. Hence, the variants provided herein both increase nanopore lifetime and improve efficiency and accuracy of DNA sequencing reactions using nanopores comprising the variants.

Abstract: Described herein are variants of alpha-hemolysin having at least one amino acid substitution at H35G, E111N, M113A, and/or K147N in the mature, wild-type alpha-hemolysin amino acid sequence. In certain examples, the variant may have a substitution at E111S, M113S, T145S, K147S, or L1351 in the mature alpha-hemolysin amino acid sequence. The ?-hemolysin variants may also include a substitution at H144A and/or a series of glycine residues spanning residues 127 to 131 of the mature, wild-type alpha hemolysin. Also provided are nanopore assemblies including the alpha-hemolysin variants, the assembly having an increased nanopore lifetime. Further, provided are variants that, in addition to providing increased lifetime, provide a decreased time-to-thread. Hence, the variants provided herein both increase nanopore lifetime and improve efficiency and accuracy of DNA sequencing reactions using nanopores comprising the variants.

Abstract: The present disclosure provides variant OmpG polypeptides, compositions comprising the OmpG variant polypeptides, and methods for using the variant OmpG polypeptides as nanopores for determining the sequence of single stranded nucleic acids. The variant OmpG nanopores reduce the ionic current noise versus the parental OmpG polypeptide from which they are derived and thereby enable sequencing of polynucleotides with single nucleotide resolution. The reduced ionic current noise also provides for the use of these OmpG nanopore variants in other single molecule sensing applications, e.g., protein sequencing.

Abstract: The present disclosure provides variant OmpG polypeptides, compositions comprising the OmpG variant polypeptides, and methods for using the variant OmpG polypeptides as nanopores for determining the sequence of single stranded nucleic acids. The variant OmpG nanopores reduce the ionic current noise versus the parental OmpG polypeptide from which they are derived and thereby enable sequencing of polynucleotides with single nucleotide resolution. The reduced ionic current noise also provides for the use of these OmpG nanopore variants in other single molecule sensing applications, e.g., protein sequencing.

Abstract: Described herein are variants of alpha-hemolysin having at least one amino acid substitution at H35G, E111N, M113A, and/or K147N in the mature, wild-type alpha-hemolysin amino acid sequence. In certain examples, the variant may have a substitution at E111S, M113S, T145S, K147S, or L135I in the mature alpha-hemolysin amino acid sequence. The ?-hemolysin variants may also include a substitution at H144A and/or a series of glycine residues spanning residues 127 to 131 of the mature, wild-type alpha hemolysin. Also provided are nanopore assemblies including the alpha-hemolysin variants, the assembly having an increased nanopore lifetime. Further, provided are variants that, in addition to providing increased lifetime, provide a decreased time-to-thread. Hence, the variants provided herein both increase nanopore lifetime and improve efficiency and accuracy of DNA sequencing reactions using nanopores comprising the variants.

Abstract: A supply magazine for use in an apparatus for removing individual film that has been placed within a light-tight pouch having a leader secured to the leading edge. The magazine has a base which receives and retains a stack of sheet film within the light-tight pouch, and a cover secured to the base which can be rotated between a closed position and an open position. A take-up spindle is rotatably secured within the base for winding of the pouch thereon. The improvement comprises a lever arm for biasing the leader toward the spindle which cooperates with hook provided on the spindle when the cover is in the closed position. The lever arm includes a clamp pad which has a configuration which forms a resistive wall section in the leader when the magazine is in the closed position such that when the spindle is rotated, the hooks will engage the leader at the resistive wall section allowing the pouch to be wound around the spindle.

Abstract: A magazine has a space for receiving a package containing a stack of film sheets enclosed within a bag. The bag is removable from the stack of sheets while the package is within the magazine by exerting a pulling force on the bag. Means are provided within the magazine for flexing the stack of film sheets in response to pulling of the bag from the stack, thus loosening adjacent sheets in the film stack that may be stuck together and facilitating removal of the sheets seriatim.

Abstract: A package for a stack of film sheets has a pouch that is sealed around the stack of sheets and a leader that is secured to the pouch and engageable with a spindle in a magazine so that rotation of the spindle can pull the pouch from around the stack of sheets. The pouch and leader are formed of different polyolefin materials with the pouch comprising a material having a low coefficient of friction to enable the pouch to be easily pulled around the stack of sheets while the leader comprises a material having a high coefficient of friction to facilitate engagement with the spindle and pulling the pouch uniformly.

Abstract: A carrier is provided for a package containing a stack of film sheets enclosed by a bag. The carrier has a first wall positionable on one side of the stack of sheets and a second wall positionable on the opposite side of the stack of sheets. The carrier has an edge wall connected to the first wall and the second wall. The edge wall has an opening at the center portion thereof, and two spaced end portions separated by the opening. The carrier allows the stack of film sheets and the carrier to flex in the area of the opening in the carrier edge wall to reduce the tendency of the sheets to stick together and thereby improve reliability of feeding of the sheets seriatim from the stack.

Abstract: A light lock is formed by a channel with spaced walls, with the channel being open at one side. A light absorbing material is positioned in the channel and has bristles projecting toward the opening. A flange projects through the opening toward the material but is spaced from the bristles. The channel can be formed on one portion of a magazine, cassette or the like, for light tight materials such as photographic film, and the flange can be formed on another portion of such a magazine or cassette.

Abstract: A supply magazine receives a package having a stack of film sheets within a light-tight bag. When the magazine is closed, a the bag is driven from the magazine so that the sheets can be removed for exposure and development. The bag is removed at the rear end of the magazine and fed toward the front of the magazine so that it is accessible to the operator and to avoid having the bag fall into an associated printer/processor and out of the reach of the operator.

Abstract: A carrier for a package containing a stack of sheets of x-ray film has a first wall located along one side of the stack of sheets and a second wall located along the opposite side of the stack of sheets. The dimensions of the walls relative to the film sheets is such that the first wall extends less than the full width of the sheets and the second wall extends less than the full length of the sheets. This enables a sheet removing device to engage and remove a sheet from either side of the stack of sheets. The package can be loaded in either of two kinds of equipment which require the package to be oriented in different ways when sheets are to be fed from the stack.

Abstract: A package has a light tight pouch that encloses a stack of rectangular sheets of film. The package can be loaded in either of two different kinds of equipment which have means for removing the pouch from the film stack so that the sheets can be fed seriatim from the stack. One kind of equipment requires the package to be oriented so that the pouch can be removed from a narrow side of the package while the second kind of equipment requires the package to be loaded therein so that the pouch can be removed from a long side of the stack of film. The pouch has flaps extending from one long side and one narrow side of the package, and the flaps are engageable by the pouch removing mechanism in the different kinds of equipment.

Abstract: A magazine receives a package having a stack of film sheets, such as x-ray film sheets, enclosed within a light-tight flexible bag. When the bag is positioned within the magazine and the magazine closed, the bag is cut and then removed from the magazine through is lightlock. A pair of stripper bars in the magazine are located between the lightlock and the stack of film sheets in the bag. The bars form a curved slot that enables the bag to be withdrawn through the slot while the bars block movement of the film sheets through the slot with the bag.