Abstract: Provided herein are methods of single-cell polypeptide and/or polynucleic acid sequencing, which facilitate the direct sequencing of a single cell without amplification. Also provided herein are compositions, kits and devices useful for the same.

Abstract: A compound represented by one of the formulae: BaaMobOc??(1), MOdPeOf??(2) or BagMohPiOj??(3) wherein for formula (1) the ratio of a:b is greater than 1:1, wherein for formula (2) the ratio of d:e is from 1:100 to 0.45:1 or from 0.55:1 to 100:1, wherein for formula (3) the ratio of g:h is from 1:7 to 1:2 and the ratio of g:i is from 1:3 to 1:1, or the ratio of g:h is from 0.6:1 to 100:1 and the ratio of g:i is from 2.2:1 to 100:1, and wherein the molybdenum present within the compound is in the 4+ oxidation state.

Abstract: A compound represented by one of the formulae: BaaMobOc??(1), MOdPeOf??(2) or BagMohPiOj??(3) wherein for formula (1) the ratio of a:b is from 1:100 to 100:1, wherein for formula (2) the ratio of d:e is from 1:100 to less than 1:1 or from greater than 1:1 to 100:1, wherein for formula (3) the ratio of g:h is from 1:100 to less than 1.5:1, wherein for formula (3) the ratio of g:i is from 1:100 to 100:1, wherein for formula (3) the ratio of h:i is from 1:100 to less than 1:2, or from greater than 1:2 to less than 2:1, or from greater than 2:1 to 100:1, and wherein the molybdenum present within the compound is in the 5+ oxidation state.

Abstract: A solid crystalline material of formula (I): AzDY4Xx, wherein each A is independently selected from Li, Na, K and Mg; D is selected from Si, Al, P, B, Ga, Ge, S, Mo, W, V, Sn, Sb, Nb and Ta, or a mixture thereof; each Y is independently selected from O, S, F, Cl, Br or a mixture thereof; each X is independently selected from F, Cl, Br, I, O, S, BH4 or a mixture thereof; z is from 2 to 8; and x is from 1 to 3. The solid crystalline material suitably provides a solid ionic conductor for use in a solid-state battery. A mixed solid crystalline material comprising the solid crystalline material, a solid-state battery comprising the solid crystalline material and a method of preparing the solid crystalline are also disclosed.

Abstract: The present disclosure relates to compounds and methods for selective C-terminal functionalization of peptides. In certain embodiments, the compounds have improved water-solubility, and are suitable for use in connection with peptide sequencing methodologies.

Abstract: A rapid diagnostic test apparatus includes: a housing; a sample chamber movably coupled to the housing; and a housing blister compartment arranged in the housing and configured to rupture when the housing is moved relative to the sample chamber from a first position to a second position. The sample chamber may include a vial portion. A vial blister compartment may seal an opening of the vial portion. The sample chamber may include a tube portion disposed in the housing. The vial portion may extend from the housing. The sample chamber may be rotatably coupled to the housing. The housing blister compartment may rupture when the housing is rotated relative to the sample chamber from the first position to the second position. The housing blister compartment may contain a fluid that may be released into the housing when in the second position.

Abstract: Diagnostic devices for performing diagnostic tests are provided, as well as methods that utilize the diagnostic devices, methods for manufacturing the diagnostic devices, and test kits for performing the diagnostic tests. The diagnostic devices may include a sample chamber; a fluid chamber connected to the sample chamber by a conduit; and a test chamber separated from the sample chamber by a breakable seal. A movable liner may form a portion of an inner surface of the sample chamber. The sample chamber may be configured to receive a sample swab, which may move in a first direction when being inserted in the sample chamber to an inserted position, and which may to move in a second direction to cause the breakable seal to break. Movement of the sample swab in the second direction may cause the liner to move in the second direction to break the breakable seal.

Abstract: Diagnostic devices for performing diagnostic tests are provided, as well as methods that utilize the diagnostic devices, methods for manufacturing the diagnostic devices, and test kits for performing the diagnostic tests. The diagnostic devices include a sample chamber with an opening for receiving a sample; a fluid chamber containing a fluid; and a test and readout chamber containing a lateral-flow assay (LFA) strip. The fluid chamber and/or the test and readout chamber is/are burstable and is/are configured to be in fluid connection with the sample chamber upon bursting. The fluid chamber may be flexible and configured to burst at a seal separating the sample chamber from the fluid chamber. The seal maybe configured to break when a bursting force is applied to the fluid chamber.

Abstract: Provided herein, in some embodiments, are rapid diagnostic tests to detect one or more target nucleic acid sequences (e.g., a nucleic acid sequence of one or more pathogens). In some embodiments, the pathogens are viral, bacterial, fungal, parasitic, or protozoan pathogens, such as SARS-CoV-2 or an influenza virus. Further embodiments provide methods of detecting genetic abnormalities. Diagnostic tests comprising a sample-collecting component, one or more reagents (e.g., lysis reagents, nucleic acid amplification reagents), and a detection component (e.g., a component comprising a lateral flow assay strip and/or a colorimetric assay) are provided.

Abstract: Methods and devices for preparing target molecules (e.g., target nucleic acids or target proteins) from a biological sample are provided herein. In some embodiments, methods and devices involve sample lysis, sample fragmentation, enrichment of target molecule(s), and/or functionalization of target molecule(s).

Abstract: Methods and devices for preparing target molecules (e.g., target nucleic acids or target proteins) from a biological sample are provided herein. In some embodiments, methods and devices involve sample lysis, sample fragmentation, enrichment of target molecule(s), and/or functionalization of target molecule(s).

Abstract: Methods and devices for preparing target molecules (e.g., target nucleic acids or target proteins) from a biological sample are provided herein. In some embodiments, methods and devices involve sample lysis, sample fragmentation, enrichment of target molecule(s), and/or functionalization of target molecule(s).

Abstract: Methods and devices for preparing target molecules (e.g., target nucleic acids or target proteins) from a biological sample are provided herein. In some embodiments, methods and devices involve sample lysis, sample fragmentation, enrichment of target molecule(s), and/or functionalization of target molecule(s).

Abstract: The present invention discloses liquid compositions that include an active compound, water, and means for inhibiting crystal formation or precipitation of the active compound in the liquid composition.

Abstract: A reagent carrier includes: a cap configured to fit on an opening of a reaction vessel; and a reagent confined by the cap. The cap may be configured to release the reagent when a user manipulates the cap while the cap is fitted on the opening of the reaction vessel. For example, the reagent may be released when the user twists the cap from a first position to a second position or when the user pushes on a surface of the cap. The cap may be configured to seal the opening of the reaction vessel when the cap is fitted on the opening of the reaction vessel. In some implementations, the cap may be a blister cap containing the reagent. In some implementations, the cap may include a cage containing the reagent. In some implementations, the cap may include a deformable structure containing the reagent.

Abstract: Provided herein, in some embodiments, are rapid diagnostic tests to detect one or more target nucleic acid sequences (e.g., a nucleic acid sequence of one or more pathogens). In some embodiments, the pathogens are viral, bacterial, fungal, parasitic, or protozoan pathogens, such as SARS-CoV-2 or an influenza virus. Further embodiments provide methods of detecting genetic abnormalities. Diagnostic tests comprising a sample-collecting component, one or more reagents (e.g., lysis reagents, nucleic acid amplification reagents), and a detection component (e.g., a component comprising a lateral flow assay strip and/or a colorimetric assay) are provided.

Abstract: Provided herein are rapid diagnostic tests, systems, and methods for detecting one or more target nucleic acid sequences (e.g., a nucleic acid sequence of one or more pathogens, such as SARS-CoV-2 or an influenza virus) using isothermal nucleic acid amplification. The tests, systems, and methods described herein may be performed in a point-of-care setting or a home setting without specialized equipment.

Abstract: The present disclosure relates to compounds and methods for selective C-terminal functionalization of peptides. In certain embodiments, the compounds have improved water-solubility, and are suitable for use in connection with peptide sequencing methodologies.

Abstract: Disclosed herein are aspects of a pulsed laser light source for producing excitation light in an integrated bioanalytical system. In some embodiments, the light source comprises one or more laser diodes that produces pulsed light signals synchronized with a common clock source for excitation of samples within reaction chambers on at least one chip. The light source may be used to provide excitation for a system with a large sensor array with reduced cost, size and electrical power requirements.

Abstract: Aspects of the application provide methods of preparing a multiplexed sample for polypeptide sequencing, and compositions, kits and devices useful for the same.