Patents by Inventor Matthew Ebersole
Matthew Ebersole has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11753620Abstract: A method and related apparatus for confirming whether a kill laser successfully destroys an undesired population of cells includes introducing fluorescent dye into cells, exciting the cells with a detection laser or a light emitting diode to cause the cell to fluoresce for a first time, measuring the amount of fluorescence in the cells with a detector capable of emitting a detection pulse, classifying the cells via embedded processing as undesired or desired cells based on the amount of fluorescence, firing a kill beam with a kill laser at any undesired cells, measuring the amount of fluorescence in the cells a second time to determine whether a fluorescent event was generated from the kill beam striking the cells, and providing feedback to an operator of the kill laser as to whether any fluorescent events were generated from the kill beam striking the cells.Type: GrantFiled: July 13, 2022Date of Patent: September 12, 2023Assignee: ABS Global, Inc.Inventors: Frederick Savage, David Appleyard, Zheng Xia, Matthew Ebersole, Daniel McAda
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Patent number: 11513114Abstract: A method of choosing which undesired cell to destroy in a multi-cell fluorescent event includes detecting fluorescence of cells, converting photons detected in the fluorescence into an analog voltage output signal, and identifying at least two discernable peaks associated with the cells. By looking solely at properties measured within the multi-cell fluorescent event, a decision of which cell to target for elimination can be made. Using this method with large population sizes can result in an effective sex skewed product. The sex skewed product can, for example, be formed from bull semen which is then later used to inseminate cows which results in an increased likelihood of giving birth to female cattle.Type: GrantFiled: May 29, 2020Date of Patent: November 29, 2022Assignee: ABS Global, Inc.Inventors: Frederick Savage, Matthew Ebersole, Daniel McAda, David Appleyard, Zheng Xia
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Publication number: 20220348865Abstract: A method and related apparatus for confirming whether a kill laser successfully destroys an undesired population of cells includes introducing fluorescent dye into cells, exciting the cells with a detection laser or a light emitting diode to cause the cell to fluoresce for a first time, measuring the amount of fluorescence in the cells with a detector capable of emitting a detection pulse, classifying the cells via embedded processing as undesired or desired cells based on the amount of fluorescence, firing a kill beam with a kill laser at any undesired cells, measuring the amount of fluorescence in the cells a second time to determine whether a fluorescent event was generated from the kill beam striking the cells, and providing feedback to an operator of the kill laser as to whether any fluorescent events were generated from the kill beam striking the cells.Type: ApplicationFiled: July 13, 2022Publication date: November 3, 2022Inventors: Frederick Savage, David Appleyard, Zheng Xia, Matthew Ebersole, Daniel McAda
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Patent number: 11427804Abstract: A method and related apparatus for confirming whether a kill laser successfully destroys an undesired population of cells includes introducing fluorescent dye into cells, exciting the cells with a detection laser or a light emitting diode to cause the cell to fluoresce for a first time, measuring the amount of fluorescence in the cells with a detector capable of emitting a detection pulse, classifying the cells via embedded processing as undesired or desired cells based on the amount of fluorescence, firing a kill beam with a kill laser at any undesired cells, measuring the amount of fluorescence in the cells a second time to determine whether a fluorescent event was generated from the kill beam striking the cells, and providing feedback to an operator of the kill laser as to whether any fluorescent events were generated from the kill beam striking the cells.Type: GrantFiled: June 14, 2019Date of Patent: August 30, 2022Assignee: ABS Global, Inc.Inventors: Frederick Hershel Savage, David Appleyard, Zheng Xia, Matthew Ebersole, Daniel McAda
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Publication number: 20220163438Abstract: Modular flow cytometry systems and methods for processing samples are described herein. The systems include automated or semi-automated modules that are replaceable and removable. A sample pathway module may be removed and placed in a microfluidic device cleaning module for cleaning, and then reinstalled or stored for later use. The systems further include optical modules, electronics modules, and mixing and collection modules. The optical module includes a photo-damaging assembly and detection laser assembly that may be on the same side relative to a plane or surface of a flow cytometry device and opposite of a detection assembly. The laser beam may have a beam waist that is wider in a direction perpendicular to a flow direction than in the flow direction. The mixing and collection module can automatically mix a sample being collected in a sample tube and switch to another sample tube when the other tube is full.Type: ApplicationFiled: November 12, 2021Publication date: May 26, 2022Inventors: Gary Klas, Daniel McAda, Zheng Xia, Matthew Ebersole, Brian Lena, David Appleyard, Richard Lu, Scott Buckley, Mark Abermoske
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Publication number: 20220117205Abstract: The present disclosure relates generally to methods for using porcine sex-sorted sperm cells for the efficient dissemination of desirable traits in multi-level swine production systems. The methods include using sex-sorted sperm cells for skewing offspring gender at the commercial farm level, producing porcine herds having improved growth performance traits, producing pathogen-resistant porcine herds, and disseminating desirable traits from a genetic nucleus to commercial farms using low dose artificial insemination techniques. The methods also provide a means for reducing costs at the production level by increasing the ratio of female offspring and improving animal welfare at all levels of production by reducing or eliminating male castration. In addition, the methods of the present technology may be employed to develop production flows for specialized pork products.Type: ApplicationFiled: October 14, 2021Publication date: April 21, 2022Inventors: Matthew CULBERTSON, Daniel HAMILTON, Matthew EBERSOLE, William HERRING
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Publication number: 20220064594Abstract: Systems and methods are provided for identification and discrimination of subpopulations within a mixture of particles. The systems and methods implement continuous calibration of the classification of particles within the mixture to provide consistency in operation and to reduce inter- and intra-batch processing variation. The systems and methods produce advantageously sorted particle products.Type: ApplicationFiled: December 21, 2018Publication date: March 3, 2022Inventors: Jim HARRINGTON, Dan ANTONUK, Fred SAVAGE, David APPLEYARD, Matthew EBERSOLE
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Publication number: 20200378953Abstract: A method of choosing which undesired cell to destroy in a multi-cell fluorescent event includes detecting fluorescence of cells, converting photons detected in the fluorescence into an analog voltage output signal, and identifying at least two discernable peaks associated with the cells. By looking solely at properties measured within the multi-cell fluorescent event, a decision of which cell to target for elimination can be made. Using this method with large population sizes can result in an effective sex skewed product. The sex skewed product can, for example, be formed from bull semen which is then later used to inseminate cows which results in an increased likelihood of giving birth to female cattle.Type: ApplicationFiled: May 29, 2020Publication date: December 3, 2020Inventors: Frederick Savage, Matthew Ebersole, Daniel McAda, David Appleyard, Zheng Xia
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Publication number: 20190382720Abstract: A method and related apparatus for confirming whether a kill laser successfully destroys an undesired population of cells includes introducing fluorescent dye into cells, exciting the cells with a detection laser or a light emitting diode to cause the cell to fluoresce for a first time, measuring the amount of fluorescence in the cells with a detector capable of emitting a detection pulse, classifying the cells via embedded processing as undesired or desired cells based on the amount of fluorescence, firing a kill beam with a kill laser at any undesired cells, measuring the amount of fluorescence in the cells a second time to determine whether a fluorescent event was generated from the kill beam striking the cells, and providing feedback to an operator of the kill laser as to whether any fluorescent events were generated from the kill beam striking the cells.Type: ApplicationFiled: June 14, 2019Publication date: December 19, 2019Inventors: Frederick Hershel Savage, David Appleyard, Zheng Xia, Matthew Ebersole, Daniel McAda
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Publication number: 20070103690Abstract: An optical probe for use in infrared, near infrared, Raman, and other spectrometers includes a probe outer surface with a cavity defined therein. The probe emits light into a sample via emission locations on the probe outer surface, at least one being in the cavity. The light emitted into the sample is then collected at collection locations which include at least two of (a) a reflectance collection location situated on the probe outer surface for collecting diffusely reflected light from any adjacent sample; (b) a transmittance collection location situated in the cavity and receiving light transmitted across the cavity from an emission location situated on an opposite side of the cavity; and (c) a transflectance collection location situated in the cavity and receiving transflected light emitted from an emission location in the cavity, with such light being reflected from a side of the cavity opposite the transflectance collection location.Type: ApplicationFiled: November 7, 2005Publication date: May 10, 2007Inventors: Matthew Ebersole, Carla Draper, Jeffrey Hirsch
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Publication number: 20070086006Abstract: A spectrometer (100) includes a light source (102) providing output light (106) to the bundled input ends (108) of multiple light pipes (110). The light pipes (110) branch into sets (118) between their input ends (108) and output ends (114), with each set (118) illuminating a sample detector (126) (via a sample chamber (122)) for measuring light scattered or emitted by a sample, or a reference detector (128) for obtaining a reference/datum measurement of the supplied light, so that comparison of measurements from the sample detector (126) and the reference detector (128) allows compensation of the sample detector measurements for drift. Efficient and accurate measurement is further assured by arraying the multiple light pipes (110) in each set (118) about the input bundle (116) so that each set receives at least substantially the same amount of light from the light source (102).Type: ApplicationFiled: October 18, 2005Publication date: April 19, 2007Inventors: Matthew Ebersole, John Iverson