Abstract: A bonnet and stuffing box assembly includes a bonnet defining a bonnet bore; a stuffing box connected to the bonnet, the stuffing box defining a stuffing box bore, the stuffing box bore and bonnet bore defining an assembly bore; a bushing assembly received in the assembly bore and comprising a stuffing box bushing and a bonnet bushing, the stuffing box bushing defining a narrow portion disposed in the stuffing box bore and a wide portion at least partially disposed in the bonnet bore, the bonnet bushing disposed in the bonnet bore and confronting the wide portion of the stuffing box bushing, the bushing assembly defining a bushing bore extending through the stuffing box bushing and the bonnet bushing; and a stem extending through the bushing bore.

Abstract: A polymerizable composition for 3D printing includes a photocurable polymer resin and metal diboride nanosheets. The resulting polymer nanocomposite includes a polymer matrix and metal diboride nanosheets dispersed throughout the polymer matrix. A method of synthesizing a nanomaterial-containing resin for 3D printing includes preparing a dispersion of metal diboride nanosheets in a solvent, and combining the dispersion with a liquid polymer resin to yield the nanomaterial-containing resin. A method of fabricating a nanocomposite structure from the nanomaterial-containing resin includes providing the nanomaterial-containing resin to a three-dimensional printer, forming a three-dimensional structure with the three-dimensional printer, and processing the three-dimensional structure to yield the nanocomposite structure.

Abstract: Example aspects of a method of using a bonnet and stuffing box assembly and a method of manufacturing a bonnet and stuffing box assembly are disclosed. The method of using a bonnet and stuffing box assembly can comprise providing a stuffing box, a bonnet, a bushing assembly, and a stem, the stuffing box and the bonnet defining an assembly bore, the bushing assembly received in the assembly bore, the bushing assembly defining a bushing bore, and the stem received through the bushing bore; sealing the stem relative to the bushing assembly to prohibit fluid flow through the bushing bore; sealing the bushing assembly relative to the bonnet to prohibit fluid flow through the assembly bore; and turning the stem relative to the bushing assembly, bonnet, and stuffing box to actuate a valve.

Abstract: Integrated diagnostic devices comprising peptide-DNA conjugates for analyte detection, an embedded biomolecular computing system for sample analysis, and a layered device architecture are provided herein. In particular, provided herein are devices comprising a layered architecture that enables diagnostic reagents, sample components, and reaction products to flow through the system with minimal user intervention.

Abstract: Example aspects of a method of using a bonnet and stuffing box assembly and a method of manufacturing a bonnet and stuffing box assembly are disclosed. The method of using a bonnet and stuffing box assembly can comprise providing a stuffing box, a bonnet, a bushing assembly, and a stem, the stuffing box and the bonnet defining an assembly bore, the bushing assembly received in the assembly bore, the bushing assembly defining a bushing bore, and the stem received through the bushing bore; sealing the stem relative to the bushing assembly to prohibit fluid flow through the bushing bore; sealing the bushing assembly relative to the bonnet to prohibit fluid flow through the assembly bore; and turning the stem relative to the bushing assembly, bonnet, and stuffing box to actuate a valve.

Abstract: Example aspects of a bushing assembly for a bonnet and stuffing box assembly, a bonnet and stuffing box assembly, and a method for using a bonnet and stuffing box assembly are disclosed. The bushing assembly for a bonnet and stuffing box assembly can comprise a stuffing box bushing, the stuffing box bushing defining an outer stuffing box bushing surface and a stuffing box bushing bore wall; and a bonnet bushing, the bonnet bushing defining an outer bonnet bushing surface and a bonnet bushing bore wall, the bonnet bushing bore wall and the stuffing box bushing bore wall together defining a bushing bore configured to receive a stem therethrough.

Abstract: Integrated diagnostic devices comprising peptide-DNA conjugates for analyte detection, an embedded biomolecular computing system for sample analysis, and a layered device architecture are provided herein. In particular, provided herein are devices comprising a layered architecture that enables diagnostic reagents, sample components, and reaction products to flow through the system with minimal user intervention.

Abstract: A polymerizable composition for 3D printing includes a photocurable polymer resin and metal diboride nanosheets. The resulting polymer nanocomposite includes a polymer matrix and metal diboride nanosheets dispersed throughout the polymer matrix. A method of synthesizing a nanomaterial-containing resin for 3D printing includes preparing a dispersion of metal diboride nanosheets in a solvent, and combining the dispersion with a liquid polymer resin to yield the nanomaterial-containing resin. A method of fabricating a nanocomposite structure from the nanomaterial-containing resin includes providing the nanomaterial-containing resin to a three-dimensional printer, forming a three-dimensional structure with the three-dimensional printer, and processing the three-dimensional structure to yield the nanocomposite structure.

Abstract: Example aspects of a bushing assembly for a bonnet and stuffing box assembly, a bonnet and stuffing box assembly, and a method for using a bonnet and stuffing box assembly are disclosed. The bushing assembly for a bonnet and stuffing box assembly can comprise a stuffing box bushing, the stuffing box bushing defining an outer stuffing box bushing surface and a stuffing box bushing bore wall; and a bonnet bushing, the bonnet bushing defining an outer bonnet bushing surface and a bonnet bushing bore wall, the bonnet bushing bore wall and the stuffing box bushing bore wall together defining a bushing bore configured to receive a stem therethrough.

Abstract: Removing heavy metal ions from an aqueous composition includes contacting an aqueous composition including a heavy metal with nanoflakes comprising MoS2 for a length of time sufficient to form nanoclusters of the heavy metal on the nanoflakes. A composite may include a porous polymeric matrix and MoS2 nanoflakes coupled to the porous polymeric matrix. Making a porous MoS2-polymer composite may include combining a solution phase dispersion of MoS2 with a polymer precursor solution to yield a mixture, treating the polymer precursor solution to yield a composite precursor, and drying the composite precursor to yield a porous MoS2-polymer composite.