Abstract: Described herein are non-viral, ex vivo methods of site-specifically inserting a transgene containing a chimeric antigen receptor (CAR) gene into a T cell genome by introducing into a population of unmodified T cells a Cas9 ribonucleoprotein (RNP) and a non-viral double-stranded homology-directed repair (HDR) template, to provide genome-edited T cells. The Cas9 ribonucleoprotein includes a Cas9 protein and a guide RNA that directs double stranded DNA cleavage of a cleavage site in a T cell expressed gene. The non-viral double-stranded HDR template comprises the synthetic DNA sequence flanked by homology arms that are complementary to sequences on both sides of the cleavage site in the T cell expressed gene. The transgene is specifically integrated into the cleavage site of the T cell expressed gene created by the Cas9 RNP in the genome-edited T cells, and the cells are then cultured.

Abstract: Advertisement vendors can be dynamically selected in response to a request from a wireless communication device. A mediation router can receive an ad request from a portal interfaced with a wireless device. Specifically, the mediation router analyzes the request and dynamically selects an ad vendor to service the request, based in part on the analysis. The selected ad vendor supplies the ad and the mediation router ensures that the portal receives the ad. The mediation router selects an ad vendor in a variety of ways including, but not limited to, business decision rules or artificial intelligence and/or a combination thereof. The mediation router further employs customer profiles that help vendors target advertisements to specific consumers and wireless devices.

Abstract: Advertisement vendors can be dynamically selected in response to a request from a wireless communication device. A mediation router can receive an ad request from a portal interfaced with a wireless device. Specifically, the mediation router analyzes the request and dynamically selects an ad vendor to service the request, based in part on the analysis. The selected ad vendor supplies the ad and the mediation router ensures that the portal receives the ad. The mediation router selects an ad vendor in a variety of ways including, but not limited to, business decision rules or artificial intelligence and/or a combination thereof. The mediation router further employs customer profiles that help vendors target advertisements to specific consumers and wireless devices.

Abstract: Advertisement vendors can be dynamically selected in response to a request from a wireless communication device. A mediation router can receive an ad request from a portal interfaced with a wireless device. Specifically, the mediation router analyzes the request and dynamically selects an ad vendor to service the request, based in part on the analysis. The selected ad vendor supplies the ad and the mediation router ensures that the portal receives the ad. The mediation router selects an ad vendor in a variety of ways including, but not limited to, business decision rules or artificial intelligence and/or a combination thereof. The mediation router further employs customer profiles that help vendors target advertisements to specific consumers and wireless devices.

Abstract: Systems and methods for providing direct shipping to mobile subscribers are disclosed. A customer ordering system receives an order request, retrieves subscriber information requested by an advertiser, and populates an order form with the retrieved information. The information can be reviewed, edited, and/or confirmed, and the order can be passed to an order fulfillment entity. Networks and devices for providing the disclosed systems and methods are also disclosed. Exemplary computer-readable media storing corresponding instructions are also disclosed. A device that executes the instructions can perform the disclosed methods and/or provide the functionality of the disclosed systems.

Abstract: Advertisement vendors can be dynamically selected in response to a request from a wireless communication device. A mediation router can receive an ad request from a portal interfaced with a wireless device. Specifically, the mediation router analyzes the request and dynamically selects an ad vendor to service the request, based in part on the analysis. The selected ad vendor supplies the ad and the mediation router ensures that the portal receives the ad. The mediation router selects an ad vendor in a variety of ways including, but not limited to, business decision rules or artificial intelligence and/or a combination thereof. The mediation router further employs customer profiles that help vendors target advertisements to specific consumers and wireless devices.

Abstract: Advertisement vendors can be dynamically selected in response to a request from a wireless communication device. A mediation router can receive an ad request from a portal interfaced with a wireless device. Specifically, the mediation router analyzes the request and dynamically selects an ad vendor to service the request, based in part on the analysis. The selected ad vendor supplies the ad and the mediation router ensures that the portal receives the ad. The mediation router selects an ad vendor in a variety of ways including, but not limited to, business decision rules or artificial intelligence and/or a combination thereof. The mediation router further employs customer profiles that help vendors target advertisements to specific consumers and wireless devices.

Abstract: Advertisement vendors can be dynamically selected in response to a request from a wireless communication device. A mediation router can receive an ad request from a portal interfaced with a wireless device. Specifically, the mediation router analyzes the request and dynamically selects an ad vendor to service the request, based in part on the analysis. The selected ad vendor supplies the ad and the mediation router ensures that the portal receives the ad. The mediation router selects an ad vendor in a variety of ways including, but not limited to, business decision rules or artificial intelligence and/or a combination thereof. The mediation router further employs customer profiles that help vendors target advertisements to specific consumers and wireless devices.

Abstract: Advertisement vendors can be dynamically selected in response to a request from a wireless communication device. A mediation router can receive an ad request from a portal interfaced with a wireless device. Specifically, the mediation router analyzes the request and dynamically selects an ad vendor to service the request, based in part on the analysis. The selected ad vendor supplies the ad and the mediation router ensures that the portal receives the ad. The mediation router selects an ad vendor in a variety of ways including, but not limited to, business decision rules or artificial intelligence and/or a combination thereof. The mediation router further employs customer profiles that help vendors target advertisements to specific consumers and wireless devices.

Abstract: A system and methodology that can dynamically select advertisement vendors in response to a request from a wireless communication device is provided. The system employ a mediation router to receive an ad request from a portal interfaced with a wireless device. Specifically, the mediation router analyzes the request and dynamically selects an ad vendor to service the request, based in part on the analysis. The selected ad vendor supplies the ad and the mediation router ensures that the portal receives the ad. The mediation router selects an ad vendor in a variety of ways including, but not limited to, business decision rules or artificial intelligence and/or a combination thereof. The mediation router further employs customer profiles that help vendors target advertisements to specific consumers and wireless devices.

Abstract: A system and methodology that can dynamically select advertisement vendors in response to a request from a wireless communication device is provided. The system employ a mediation router to receive an ad request from a portal interfaced with a wireless device. Specifically, the mediation router analyzes the request and dynamically selects an ad vendor to service the request, based in part on the analysis. The selected ad vendor supplies the ad and the mediation router ensures that the portal receives the ad. The mediation router selects an ad vendor in a variety of ways including, but not limited to, business decision rules or artificial intelligence and/or a combination thereof. The mediation router further employs customer profiles that help vendors target advertisements to specific consumers and wireless devices.

Abstract: Systems and methods for providing direct shipping to mobile subscribers are disclosed. A customer ordering system (COS) can receive an order request, determine the information requested by an advertiser, retrieve the information requested, and populate an order form with the retrieved information. The information can be reviewed, edited, and/or confirmed, and the order can be passed to an order fulfillment entity. Networks and devices for providing the disclosed systems and methods are also disclosed. Exemplary computer readable media for storing instructions are also disclosed. A device that executes the instructions can perform the disclosed methods and/or provided the functionality of the disclosed systems.

Abstract: A device and method for measuring optical properties of a sample are provided. The device comprises a housing surrounding a flow-through flow-cell. The flow-cell has a sample inlet positioned proximate to a first end of the flow-cell and a sample outlet positioned proximate to a second end of the flow-cell. A sample chamber is positioned between the sample inlet and the sample outlet. A plurality of excitation sources are positioned on the housing in an operable relationship to the sample chamber. At least one excitation source has a wavelength that is different from the other excitation sources. Each excitation source is incident on a sample in the flow-cell. At least one fluorescence emission detector is positioned in an operable relationship to the flow-cell. The fluorescence emission detector detects a continuous broadband spectrum of emission wavelengths emitted from the sample upon excitation by each excitation source.

Abstract: A metal-air cell including (a) a container having a positive end and a negative end; (b) a cathode having an end adjacent to the positive end of the container and an end adjacent to the negative end of the container; (c) an anode; (d) a separator between the cathode and the anode; and (e) a seal between the end of the cathode adjacent to the positive end of the container and the positive end of the container is disclosed. The seal includes a hot melt material.