Abstract: The use of matrix-assisted laser desorption ionization (MALDI) mass spectrometry imaging (MSI) to assay feline tissue samples is described. Methods of analyzing a tissue sample may generally comprise generating sample ions directly from the tissue sample using a MALDI ionization source, receiving the ions into a mass spectrometer, identifying at least one inflammatory bowel disease and/or lymphoma related compound in the sample from results from the mass spectrometer, comparing the at least one identified related compound in the sample to one or more known inflammatory bowel disease and/or lymphoma profiles, and identifying at least one condition related to the sample from the comparison of the at least one identified related compound to the one or more known inflammatory bowel disease and/or lymphoma profiles. The mass spectrometer may be a quadrupole mass spectrometer, a time of flight mass spectrometer, an Orbitrap mass spectrometer, or an ion trap mass spectrometer.

Abstract: A compound may generally comprise the formula: wherein R1 is independently selected from C2-C10 alkyl or substituted alkyl, R2 is independently selected from the group consisting of —H and C1-C6 alkyl or substituted alkyl, X is selected from the group consisting of —NH— and —O—, Y is a carbohydrate, and m is an integer from 1 to 8. The compound may comprise a non-ionic acid labile surfactant. The compound may be used to facilitate solubilization of proteins and other molecules in an aqueous environment.

Abstract: A compound may generally comprise the formula: wherein R1 is independently selected from C2-C10 alkyl or substituted alkyl, R2 is independently selected from the group consisting of —H and C1-C6 alkyl or substituted alkyl, X is selected from the group consisting of —NH— and —O—, Y is a carbohydrate, and m is an integer from 1 to 8. The compound may comprise a non-ionic acid labile surfactant. The compound may be used to facilitate solubilization of proteins and other molecules in an aqueous environment.

Abstract: A compound may generally comprise the formula: wherein R1 is independently selected from C2-C10 alkyl or substituted alkyl, R2 is independently selected from the group consisting of —H and C1-C6 alkyl or substituted alkyl, X is selected from the group consisting of —NH— and —O—, Y is a carbohydrate, and m is an integer from 1 to 8. The compound may comprise a non-ionic acid labile surfactant. The compound may be used to facilitate solubilization of proteins and other molecules in an aqueous environment.

Abstract: Anionic acid-labile surfactants may generally comprise compounds represented by the formula: wherein R1 is independently selected from —(CH2)0-9CH3, R2 is selected from the group consisting of —H and —(CH2)0-5CH3, Y is an anion, X is a cation, and n is an integer from 1 to 8. Methods of making and using the anionic acid-labile surfactants are also described. The anionic acid-labile surfactants may be used to facilitate the solubilization of proteins and other molecules in an aqueous environment.

Abstract: Anionic acid-labile surfactants may generally comprise compounds represented by the formula: wherein R1 is independently selected from —(CH2)0-9CH3, R2 is selected from the group consisting of —H and —(CH2)0-5CH3, Y is an anion, X is a cation, and n is an integer from 1 to 8. Methods of making and using the anionic acid-labile surfactants are also described. The anionic acid-labile surfactants may be used to facilitate the solubilization of proteins and other molecules in an aqueous environment.

Abstract: A compound may generally comprise the formula: wherein R1 is independently selected from C2-C10 alkyl or substituted alkyl, R2 is independently selected from the group consisting of —H and C1-C6 alkyl or substituted alkyl, X is selected from the group consisting of —NH— and —O—, Y is a carbohydrate, and m is an integer from 1 to 8. The compound may comprise a non-ionic acid labile surfactant. The compound may be used to facilitate solubilization of proteins and other molecules in an aqueous environment.

Abstract: Anionic acid-labile surfactants may generally comprise compounds represented by the formula: wherein R1 is independently selected from —(CH2)0-9CH3, R2 is selected from the group consisting of —H and —(CH2)0-5CH3, Y is an anion, X is a cation, and n is an integer from 1 to 8. Methods of making and using the anionic acid-labile surfactants are also described. The anionic acid-labile surfactants may be used to facilitate the solubilization of proteins and other molecules in an aqueous environment.

Abstract: Anionic acid-labile surfactants may generally comprise compounds represented by the formula: wherein R1 is independently selected from —(CH2)0-9CH3, R2 is selected from the group consisting of —H and —(CH2)0-5CH3, Y is an anion, X is a cation, and n is an integer from 1 to 8. Methods of making and using the anionic acid-labile surfactants are also described. The anionic acid-labile surfactants may be used to facilitate the solubilization of proteins and other molecules in an aqueous environment.

Abstract: Anionic acid-labile surfactants may generally comprise compounds represented by the formula: wherein R1 is independently selected from —(CH2)0-9CH3, R2 is selected from the group consisting of —H and —(CH2)0-5CH3, Y is an anion, X is a cation, and n is an integer from 1 to 8. Methods of making and using the anionic acid-labile surfactants are also described. The anionic acid-labile surfactants may be used to facilitate the solubilization of proteins and other molecules in an aqueous environment.

Abstract: Anionic acid-labile surfactants may generally comprise compounds represented by the formula: wherein R1 is independently selected from —(CH2)0-9CH3, R2 is selected from the group consisting of —H and —(CH2)0-5CH3, Y is an anion, X is a cation, and n is an integer from 1 to 8. Methods of making and using the anionic acid-labile surfactants are also described. The anionic acid-labile surfactants may be used to facilitate the solubilisation of proteins and other molecules in an aqueous environment.

Abstract: A compound of the formula: wherein R1 is independently selected from C1-C10 alkyl or substituted alkyl, R2 is independently selected from the group consisting of —H and C1-C6 alkyl or substituted alkyl, Y? is an anion, m is an integer from 1 to 8 and n is an integer from 1 to 8 is described. Methods of making and using the compound are also described. The compound may comprise a zwitterionic acid-labile surfactant suitable for purification and identification techniques used in proteomics.

Abstract: Anionic acid-labile surfactants may generally comprise compounds represented by the formulas: and wherein R1 is independently selected from —(CH2)0-9CH3, R2 is selected from the group consisting of —H and —(CH2)0-5CH3, Y is an anion, X is a cation, and n is an integer from 1 to 8. Methods of making and using the anionic acid-labile surfactants are also disclosed.

Abstract: Anionic acid-labile surfactants may generally comprise compounds represented by the formula: wherein R1 is independently selected from —(CH2)0-9CH3, R2 is selected from the group consisting of —H and —(CH2)0-5CH3, Y is an anion, X is a cation, and n is an integer from 1 to 8.

Abstract: A microfluidic device for electroelution with sample collection decoupled from the electrophoretic field can generally comprise a channel having a first fluid pathway in fluid communication with a second fluid pathway, the first fluid pathway can comprise a first port in fluid communication with a second port, and a receptacle intermediate the ports, the second fluid pathway can comprise an inlet in fluid communication with an outlet, the first and second ports can be associated with first and second electrodes, respectively, such that the electrodes can create an electrophoretic field across the receptacle, and the channel can be configured to create a pressure drop from the first fluid pathway towards the second fluid pathway that encourages the electroeluted sample to flow towards the second fluid pathway.

Abstract: A microfluidic device for collecting a sample during electrokinetic transport may generally comprise a first channel intersecting a second channel to form a junction; a receptacle in fluid communication with the first channel to receive therein a sample comprising at least one analyte; a pair of electrodes associated with the first channel to create an electrophoretic field effective to electrokinetically transport the at least one analyte, wherein the second channel is substantially field-free of the electrophoretic field; and a first reservoir and a second reservoir in fluid communication with the first channel to create a pressure gradient between the channels effective to transport the at least one analyte from the first channel to the second channel when fluid is present in at least one of the reservoirs and the voltage is substantially simultaneously applied along the first channel.