Abstract: The present disclosure provides engineered adenylate kinases, and recombinant polynucleotides encoding the engineered adenylate kinases. The present disclosure further provides uses of the engineered adenylate kinases for converting NMP to NDP.

Abstract: A method for remotely signalling a downhole assembly comprising one or more downhole tools includes operating a choke arrangement configured to control production fluid flow from a wellbore to produce a first pressure signature in the fluid flow, the first pressure signature defining a trigger signal for the one or more downhole tools of the downhole assembly. The first pressure signature comprises at least a first pressure signal and a second pressure signal in the production fluid flow in the downhole assembly. Operating the choke arrangement to produce a second pressure signature in the production fluid flow in the downhole assembly at a predetermined time period following the first pressure signature, the second pressure signature defining a command signal for initiating operation of a predetermined one or more of the downhole tools of the downhole assembly.

Abstract: The present disclosure relates to engineered RNA polymerase polypeptides and compositions thereof, as well as polynucleotides encoding the engineered RNA polymerase polypeptides. The present disclosure also provides methods of using the engineered RNA polymerase polypeptides or compositions thereof for producing RNA.

Abstract: The present invention provides improved P450-BM3 variants with improved activity. In some embodiments, the P450-BM3 variants exhibit improved activity on a 1-tert-Butoxycarbonylaminocyclopentanoic acid substrate.

Abstract: The present disclosure relates to engineered DNA ligase polypeptides and compositions thereof, as well as polynucleotides encoding the engineered DNA ligase polypeptides. The present disclosure also provides methods of using the engineered DNA ligase polypeptides or compositions thereof in diagnostics and as a molecular biological tool.

Abstract: The present invention provides engineered 3?O-kinase polypeptides useful for construction of materials used in template-independent polynucleotide synthesis, as well as compositions and methods of utilizing these engineered polypeptides. The present disclosure also describes one-pot methods for conversion of a natural or modified nucleoside to a nucleoside tetraphosphate or NQP.

Abstract: The present disclosure provides enzymatic methods for the production of 3?-phosphate-nucleoside-5?-triphosphate (NQP). The present disclosure further provides enzymatic methods for the production of nucleotide-5?-triphosphates.

Abstract: The present invention provides improved P450-BM3 variants with improved activity. In some embodiments, the P450-BM3 variants exhibit improved activity on indanone substrates.

Abstract: The present invention provides engineered uridine phosphorylase (UP) enzymes, polypeptides having UP activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. Methods for producing UP enzymes are also provided. The present invention further provides compositions comprising the UP enzymes and methods of using the engineered UP enzymes. The present invention finds particular use in the production of pharmaceutical compounds.

Abstract: A robotic surgical instrument includes an articulation drivable by at least one driving element. Movement of an instrument interface element is transferred to the at least one driving element. A driving element securing member includes at least one tapered side wall and is configured to be coupled to the at least one driving element. A holding member includes an opening configured to receive a fixing member and at least one wall defining a recess having a shape complementary to the shape of the at least one tapered side wall. The fixing member is configured to be received in the opening and apply force to secure the driving element securing member within the recess of the holding member so that the at least one wall of the holding member is in frictional contact with the tapered side wall of the driving element securing member.

Abstract: The present invention provides engineered uridine phosphorylase (UP) enzymes, polypeptides having UP activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. Methods for producing UP enzymes are also provided. The present invention further provides compositions comprising the UP enzymes and methods of using the engineered UP enzymes. The present invention finds particular use in the production of pharmaceutical compounds.

Abstract: The present invention relates to plant-produced angiogenins, to related plant cells, plant calli, plants, seeds and other plant parts and products derived therefrom and to uses of plant-produced angiogenins. The present invention also relates to expression of angiogenin genes in plants and to related nucleic acids, constructs and methods.

Abstract: A system has data capture devices collecting data from different points in a network. The captured data is written to a data store and is directed to an output. The data from the different data capture devices can be delivered to a data analytics device. As long as the data analytics device is able to keep pace with the data that is directed to the output, that data is used by the analytics device. If the analytics device is not able to keep pace, the data written to the data store is retrieved and is used until the analytics device has caught up.

Abstract: A method for remotely signalling a downhole assembly comprising one or more downhole tools includes operating a choke arrangement configured to control production fluid flow from a wellbore to produce a first pressure signature in the fluid flow, the first pressure signature defining a trigger signal for the one or more downhole tools of the downhole assembly. The first pressure signature comprises at least a first pressure signal and a second pressure signal in the production fluid flow in the downhole assembly. Operating the choke arrangement to produce a second pressure signature in the production fluid flow in the downhole assembly at a predetermined time period following the first pressure signature, the second pressure signature defining a command signal for initiating operation of a predetermined one or more of the downhole tools of the downhole assembly.

Abstract: The present invention relates to plant-produced angiogenins, to related plant cells, plant calli, plants, seeds and other plant parts and products derived therefrom and to uses of plant-produced angiogenins. The present invention also relates to expression of angiogenin genes in plants and to related nucleic acids, constructs and methods.

Abstract: The present invention relates to plant-produced angiogenins, to related plant cells, plant calli, plants, seeds and other plant parts and products derived therefrom and to uses of plant-produced angiogenins. The present invention also relates to expression of angiogenin genes in plants and to related nucleic acids, constructs and methods.

Abstract: The present invention provides engineered terminal deoxynucleotidyl transferase (TdT) polypeptides useful in template-independent polynucleotide synthesis using a nucleoside triphosphate-3?-O-removable blocking group (NTP-3?-O-RBG), as well as compositions, methods of utilizing these engineered polypeptides, and polynucleotides encoding the engineered terminal deoxynucleotidyl transferases.

Abstract: A surgical robot system comprising: a surgical robot, the surgical robot comprising a base, and an arm extending from the base to an attachment for an instrument the arm comprising a plurality of joints whereby the configuration of the arm can be altered; an instrument attached to the arm of the surgical robot; and a control unit configured to receive one or more inputs from an operator of the surgical robot in relation to movement of the instrument and translate the one or more inputs into one or more control signals in accordance with a set of control parameters to control the movement of the instrument, the set of control parameters being one of one or more sets of control parameters associated with the operator.

Abstract: There is provided a flavour system for use in a tobacco industry product. The flavour system comprises lyophilised botanical material, an activator capable of releasing flavourant from the lyophilised botanical material through contact of the activator with the lyophilised botanical material, and a removable barrier preventing contact between the lyophilised botanical material and the activator. The barrier is removable to permit the activator to contact the lyophilised botanical material, thereby to cause release of the flavourant.

Abstract: The present invention provides engineered uridine phosphorylase (UP) enzymes, polypeptides having UP activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. Methods for producing UP enzymes are also provided. The present invention further provides compositions comprising the UP enzymes and methods of using the engineered UP enzymes. The present invention finds particular use in the production of pharmaceutical compounds.