Abstract: A sustainable, prefabricated building system, including prefabricated wall panels, load bearing beams, and prefabricated roof panels, and methods of assembly are presented herein. In one aspect, a prefabricated building assembly includes a number of prefabricated wall panel subassemblies mounted to a concrete foundation with a desired offset distance between the bottom of each prefabricated wall panel assembly and the foundation. Each prefabricated wall panel includes a metal base angle subframe coupled to structural wood subassembly. In another aspect, a metal base angle subframe includes end caps welded to each end of the structural angle to facilitate weather proofing and isolation of the wall panel assembly from the ground. In some embodiments, a wall panel subassembly includes any of an interior facing finishing layer, one or more utility chases, a weather resistive membrane, an insulation layer, one or more flashing elements, and an exterior finishing layer.

Abstract: Compositions and methods are provided for amplifying nucleic acids, including cell free nucleic acid fragments, in preparation for sequencing. Methods are provided for making circularized nucleic acid templates having the structure [T]-[PS1]-[L]-[PS2] or [PS1]-[L]-[PS2]-[T?], where (a) T is a target nucleic acid and T? is a complement to a target nucleic acid; (b) each of PS1 and PS2 is a nucleic acid primer site; (c) L is a linker having a primer extension reaction terminating organic molecule; and the structure is circularized by binding a 5? end thereof to a 3? end thereof. Target sequences in the circularized templates are amplified by binding to PS1 a primer complimentary to PS1 and binding to PS2 a primer complimentary to PS2 and copying the target sequences by a primer extension reaction. Advantages include a reduction in ligation steps, which can result in fewer clean up steps and improved library conversion efficiency.

Abstract: In various aspects, the present disclosure provides methods, compositions, reactions mixtures, kits, and systems for analysis of cell-free nucleic acid molecules (e.g., cfRNA and/or cfDNA) from a urine sample. In some embodiments, the analysis is an analysis of methylation patterns in target genomic regions among cfDNA fragments in a urine sample. In some embodiments, compositions include a plurality of different bait oligonucleotides. Methods for the detection of cancer of various cancer types are also provided.

Abstract: Methods for measuring subpopulations of target molecules (e.g., polypeptides and/or cell-free ribonucleic acid) are provided. In some embodiments, methods of generating a sequencing library from a plurality of RNA molecules in a test sample obtained from a subject are provided, as well as methods for analyzing the sequencing library to detect, e.g., the presence or absence of a disease.

Abstract: Methods for measuring subpopulations of ribonucleic acid (RNA) molecules are provided. In some embodiments, methods of generating a sequencing library from a plurality of RNA molecules in a test sample obtained from a subject are provided, as well as methods for analyzing the sequencing library to detect, e.g., the presence or absence of a disease.

Abstract: Methods for measuring subpopulations of ribonucleic acid (RNA) molecules are provided. In some embodiments, methods of generating a sequencing library from a plurality of RNA molecules in a test sample obtained from a subject are provided, as well as methods for analyzing the sequencing library to detect, e.g., the presence or absence of a disease.

Abstract: In various aspects, the present disclosure provides methods, compositions, reactions mixtures, kits, and systems for sequencing both RNA and DNA from a single source sample. In some embodiments, RNA is treated so as to differentiate RNA sequences from DNA sequences derived from the same sample. In some embodiments, the RNA and DNA are cell-free polynucleotides.

Abstract: Methods for measuring subpopulations of cell-free ribonucleic acid (RNA) molecules are provided. In some embodiments, methods of generating a sequencing library from a plurality of RNA molecules in a test sample obtained from a subject are provided, as well as methods for analyzing the sequencing library to detect, e.g., the presence or absence of a disease.

Abstract: In various aspects, the present disclosure provides methods, compositions, reactions mixtures, kits, and systems for sequencing both RNA and DNA from a single source sample. In some embodiments, RNA is treated so as to differentiate RNA sequences from DNA sequences derived from the same sample. In some embodiments, the RNA and DNA are cell-free polynucleotides.

Abstract: Aspects of the invention include methods for preparing sequencing libraries comprising a plurality of RNA molecules. In some embodiments, the subject methods comprise sequencing at least a portion of a sequencing library to obtain sequencing data or sequence reads from a test sample (e.g., a biological sample from a subject).

Abstract: Methods for measuring subpopulations of ribonucleic acid (RNA) molecules are provided. In some embodiments, methods of generating a sequencing library from a plurality of RNA molecules in a test sample obtained from a subject are provided, as well as methods for analyzing the sequencing library to detect, e.g., the presence or absence of a disease.

Abstract: In various aspects, the present disclosure provides methods, compositions, reactions mixtures, kits, and systems for sequencing both RNA and DNA from a single source sample. In some embodiments, RNA is treated so as to differentiate RNA sequences from DNA sequences derived from the same sample. In some embodiments, the RNA and DNA are cell-free polynucleotides.

Abstract: In various aspects, the present disclosure provides methods, compositions, reactions mixtures, kits, and systems for sequencing both RNA and DNA from a single source sample. In some embodiments, RNA is treated so as to differentiate RNA sequences from DNA sequences derived from the same sample. In some embodiments, the RNA and DNA are cell-free polynucleotides.

Abstract: Aspects of the invention include methods for preparing sequencing libraries comprising a plurality of RNA molecules. In some embodiments, the subject methods comprise sequencing at least a portion of a sequencing library to obtain sequencing data or sequence reads from a test sample (e.g., a biological sample from a subject).

Abstract: In various aspects, the present disclosure provides methods, compositions, reactions mixtures, kits, and systems for sequencing both RNA and DNA from a single source sample. In some embodiments, RNA is treated so as to differentiate RNA sequences from DNA sequences derived from the same sample. In some embodiments, the RNA and DNA are cell-free polynucleotides.

Abstract: Embodiments relate to systems and methods for behavior-based automated malware analysis and classification. Aspects relate to platforms and techniques which access a set of samples of malware, and extract or capture a set of low-level behavioral artifacts produced by those samples. The low-level artifacts can be used to organize or identify a set of features, based upon which the sample can be classified and/or clustered into different labels, groups, or categories. The artifacts and/or features can be analyzed by one or more selectable algorithms, whose accuracy, efficiency, and other characteristics can be compared to one another for purposes of performing a classification or clustering task. The algorithm(s) can be selected by a user to achieve desired run times, accuracy levels, and/or other effects.

Abstract: Embodiments relate to systems and methods for behavior-based automated malware analysis and classification. Aspects relate to platforms and techniques which access a set of samples of malware, and extract or capture a set of low-level behavioral artifacts produced by those samples. The low-level artifacts can be used to organize or identify a set of features, based upon which the sample can be classified and/or clustered into different labels, groups, or categories. The artifacts and/or features can be analyzed by one or more selectable algorithms, whose accuracy, efficiency, and other characteristics can be compared to one another for purposes of performing a classification or clustering task. The algorithm(s) can be selected by a user to achieve desired run times, accuracy levels, and/or other effects.

Abstract: This is a security and identification system designed to obtain improved images for use by real-time facial recognition and identification systems for screening individuals passing through secure entry or checkpoints such as airport passenger terminals, government offices, and other secure locations. The system detects the presence of a subject at the checkpoint, interactively instructs the subject to stop and move into position for proper identification and recognition, analyzes the facial features of the subject as (s)he passes through the checkpoint, and compares the features of the subject with those in a database. The system then generates different signals depending upon whether or not the subject is recognized. In one aspect of the invention, different methods and apparatus are provided for compensating for low ambient light so as to improve the quality of the facial image of the subject that is obtained.

Abstract: The function polling system both uses the proprietary reporting Application Interface of the software application, as needed, while also bypassing the proprietary reporting Application Interface to directly poll the executing software application to perform a time-wise snapshot of the executing software application to thereby limit the amount of data generated, yet provide a more comprehensive view of the executing software application. The function polling system periodically checks the software application to determine which function is presently executing within the software application. The periodic polling of executing processes significantly reduces the amount of data collected, yet enables the application stakeholder to obtain a granularity of data relating to the executing software application not available with existing software monitoring systems.

Abstract: This is a security and identification system designed to obtain improved images for use by real-time facial recognition and identification systems for screening individuals passing through secure entry or checkpoints such as airport passenger terminals, government offices, and other secure locations. The system detects the presence of a subject at the checkpoint, interactively instructs the subject to stop and move into position for proper identification and recognition, analyzes the facial features of the subject as (s)he passes through the checkpoint, and compares the features of the subject with those in a database. The system then generates different signals depending upon whether or not the subject is recognized. In one aspect of the invention, different methods and apparatus are provided for compensating for low ambient light so as to improve the quality of the facial image of the subject that is obtained.