Abstract: Methods and compositions are provided for rapidly identifying novel structure-switching aptamers.

Abstract: Methods and compositions are provided for identifying novel ligands for a protein target.

Abstract: Aptamers targeted to a human transferrin receptor which do not compete with transferrin for binding are provided. Compositions and methods for aptamer-targeted liposomal drug delivery are also provided.

Abstract: The present invention is a method, system and apparatus for flexible image sharing. In a system for flexibly distributing imagery in an image sharing session, an image cache can be disposed between an application sharing host and a plurality of application sharing viewers. Additionally, a multipoint communications server can be disposed between the image cache and the application sharing viewers and can be communicatively linked to the application sharing host. Importantly, a pull-based subscription model can be implemented in an image cache server hosting the image cache. In particular, the model can have a configuration for registering respective ones of the application sharing viewers to receive notifications of available updated imagery.

Abstract: A coating process is provided. The coating process includes positioning a component within an enclosed coating system, the enclosed coating system including a coating apparatus, applying a first coating over at least a portion of the component, then robotically applying a mask to the component, then applying a second coating over at least the portion of the component. The component remains within the enclosed coating system throughout the process. Another process also includes removing a first coating head portion of the coating apparatus after applying the first coating and replacing the first coating head portion with a masking head portion, then removing the masking head portion after robotically applying the mask to the component and replacing the masking head portion with a second coating head portion.

Abstract: Turbine component internal heating systems include at least one turbine component support platform that supports a turbine component having one or more internal cavities by temporarily engaging at least a side wall of the turbine component, and, at least one heat source that extends from the at least one turbine component support platform, wherein when the at least one turbine component support platform supports the turbine component, the at least one heat source is at least partially disposed within at least one of the one or more internal cavities such that it can heat the turbine component from the inside.

Abstract: A composition is provided comprising an oligonucleotide aptamer conjugated to an antigen, wherein the aptamer is directed against a cell-surface target of an antigen-presenting cell. Also provided are methods of delivering an antigen to a dendritic cell and of eliciting an immune response in a subject.

Abstract: A method of delivering a cargo agent into cytosol of a cell can include: providing the delivery system of one of the embodiments described herein having the first and second delivery platforms; and administering the delivery system to a cell so as to cause targeting of two features on the cell so as to: cause endocytosis of the first and second delivery platforms of the delivery system into a common endosome, destabilize the endosome of the cell having the delivery system, release the cargo agent from the second linker; and release the cargo agent from the destabilized endosome into cytosol of the cell. A method of treating a disease can include: performing the method of method of delivering a cargo agent into cytosol of a cell in a subject having a disease, wherein the cargo agent is a therapeutic agent for the disease.

Abstract: Aptamers targeted to a human transferrin receptor which do not compete with transferrin for binding are provided. Compositions and methods for aptamer-targeted liposomal drug delivery are also provided.

Abstract: The present invention addresses a need for improved vehicles for delivering small interfering RNAs (siRNAs).

Abstract: The present application provides a turbine component for use in a hot gas path of a gas turbine. The turbine component may include an outer surface, an internal cooling circuit, a number of cooling pathways in communication with the internal cooling circuit and extending through the outer surface, and a number of adaptive cooling pathways in communication with the internal cooling circuit and extending through the outer surface. The adaptive cooling pathways may include a high temperature compound therein.

Abstract: The present invention is a method, system and apparatus for flexible image sharing. In a system for flexibly distributing imagery in an image sharing session, an image cache can be disposed between an application sharing host and a plurality of application sharing viewers. Additionally, a multipoint communications server can be disposed between the image cache and the application sharing viewers and can be communicatively linked to the application sharing host. Importantly, a pull-based subscription model can be implemented in an image cache server hosting the image cache. In particular, the model can have a configuration for registering respective ones of the application sharing viewers to receive notifications of available updated imagery.

Abstract: A method for coating a component and forming at least one cooling hole in a component is provided. The method includes providing the component having a surface and including a plurality of apertures formed therein. The method includes masking at least one of the plurality of apertures with a plug comprising a removable material. The method includes applying at least one coating to the surface of the component. The method includes eliminating the plug including removable material leaving open apertures in the surface of the coated component. Also provided is a water-soluble aperture plug including water soluble high temperature resistant filler materials including aluminum oxide, zirconium oxide, magnesium oxide, silicon dioxide, zircon, graphite, tungsten carbide, silicon carbide, silicon nitride, boron nitride, aluminum nitrides and binding agents and dispersants including phosphates, silicates, sugar, salt, gum, resin, polyvinyl alcohol (PVA), polyethylene glycol, and combinations thereof.

Abstract: The present invention is a method, system and apparatus for flexible image sharing. In a system for flexibly distributing imagery in an image sharing session, an image cache can be disposed between an image sharing host and a plurality of image sharing viewers. Additionally, a multipoint communications server can be disposed between the image cache and the image sharing viewers and can be communicatively linked to the image sharing host. Importantly, a pull-based subscription model can be implemented in an image cache server hosting the image cache. In particular, the model can have a configuration for registering respective ones of the image sharing viewers to receive notifications of available updated imagery. In a preferred aspect of the present invention, the image sharing host can be an application sharing host hosting a shared application among the image sharing viewers.

Abstract: The present invention provides methods of preparing an oligonucleotide, nucleoside or nucleoside analog for selective introduction into a subject's cells, the method comprising (1) selecting a targeted aptamer, internalizing nucleic acid or tumor-homing nucleic acid via iterative rounds of selection, and (i) hybridizing it to an oligonucleotide, (ii) replacing one or more nucleotide with a nucleoside or nucleoside analog, or (iii) synthesizing the it with one or more nucleoside or nucleoside analogs; or (2) preparing a naive combinatorial aptamer, internalizing nucleic acid or tumor-homing nucleic acid prodrug library, and running iterative rounds of selection for the cells. The present invention also provides the agent, the pharmaceutical composition, and methods of treating or preventing cancer and/or viral infection, the method comprising administration of the oligonucleotide, nucleoside or nucleoside analog for selective introduction into a subject's cells.

Abstract: The present invention includes compositions and methods for contacting one or more cells with a random RNA-containing library, treating the contacted cells and with a denaturing agent or digestion with one or more nucleases, and extracting from the cells one or more internalized nucleic acids resistant to the nucleases or denaturants.

Abstract: The present invention includes compositions and methods for contacting one or more cells with a random RNA-containing library, treating the contacted cells and with a denaturing agent or digestion with one or more nucleases, and extracting from the cells one or more internalized nucleic acids resistant to the nucleases or denaturants.

Abstract: The present invention relates to compositions and methods for sensitive, rapid and convenient assays to detect and/or quantify one or more target using ribonucleic acid as probes, wherein the method includes binding a first and a second ribonucleic acid probe, each of which binds specifically to the target, wherein the first and second probes each comprise a ribonucleic acid tail; ligating the first and second ribonucleic acids tails thereby producing a ligated ribonucleic acid template; and performing amplification of the ribonucleic acid template across the first and second ribonucleic acids.

Abstract: The present invention includes compositions and methods for the detection of specific targets on a surface that includes one or more peptides and one or more oligonucleotides connected by a joint to a detectable marker, wherein the joint between the peptides, the oligonucleotides or both the peptides and oligonucleotides are immobilized.

Abstract: The present invention is a method, system and apparatus for flexible image sharing. In a system for flexibly distributing imagery in an image sharing session, an image cache can be disposed between an image sharing host and a plurality of image sharing viewers. Additionally, a multipoint communications server can be disposed between the image cache and the image sharing viewers and can be communicatively linked to the image sharing host. Importantly, a pull-based subscription model can be implemented in an image cache server hosting the image cache. In particular, the model can have a configuration for registering respective ones of the image sharing viewers to receive notifications of available updated imagery. In a preferred aspect of the present invention, the image sharing host can be an application sharing host hosting a shared application among the image sharing viewers.