Abstract: Systems and methods for preparing enriched biological samples, and methods of use thereof are described herein. The systems can include a containment device connected to a first insert to collect tissue fragments in the containment device or to a second insert to incubate tissue fragments with a blood sample in the containment device. The methods can include methods for preparing a cytokine or growth factor-enriched biological sample, such as IL-1 Ra-enriched serum, methods of treating inflammation, inflammatory joint, or rheumatoid arthritis, and methods of preventing inflammation of a joint following arthroscopic surgery.

Abstract: An anti-rotation sleeve for an electric brake system having a shaft received in and movable relative to a passage of a housing includes a body extending along a centerline from a first end to a second end. An inner surface defines a passage for receiving the shaft and pockets extending radially from the passage configured to provide sliding interfaces with tabs on the shaft. Projections provided on an outer surface of the body and extending parallel to the centerline cooperate with the housing to prevent rotation of the sleeve relative to the housing. Each projection is aligned with a normal direction of one of the interfaces.