Abstract: Provided herein are delivery particle systems (XDP) useful for the delivery of payloads of any type. In some embodiments, a XDP particle system with tropism for target cells of interest is used to deliver CRISPR/Cas polypeptides (e.g., CasX proteins) and guide nucleic acids (gNA), for the modification of nucleic acids in target cells. Also provided are methods of making and using such XDP to modify the nucleic acids in such cells.

Abstract: In one or more implementations, body features of a subject can be tracked and a pose estimation produced using an unconstrained video sequence. The video sequence constitutes a physical record of the body features of the subject. A set of 2D coordinates corresponding to the body features of the subject can be received from a first neural network and 2D Keypoints are detected and transmitted to a second neural network. The second neural network can return corresponding depth estimation values, which can be processed to generate a set of 3D coordinates that correspond to the body features of the subject in camera space. Furthermore, one or more non-linear optimizations can be applied to fit a predetermined skeleton to the generate 3D Keypoints, and produce the pose estimation corresponding to the body features of the subject.

Abstract: A method for locating and tracking facial features in an unconstrained video sequence includes: in a face-detecting process, delineating, with region-bounding coordinates, the face of the subject within an image selected from the sequence; detecting, in the selected image, a small set of landmarks, corresponding to the face of the subject, using a convolutional neural network, trained to take as input an image region corresponding to the face of the—subject and to return a set of coordinates at computational speeds approximating real time; projectively fitting a three-dimensional character model to the detected landmarks, and using the fitted model to estimate physical locations of additional landmarks, so as to provide a complete hypothesized set of facial landmarks; and in a feature tracker process, updating the hypothesized set of facial landmarks to improve convergence between predicted feature locations and their actual physical locations based on data sampled from the selected image.

Abstract: A method for locating and tracking facial features in an unconstrained video sequence includes: in a face-detecting process, delineating, with region-bounding coordinates, the face of the subject within an image selected from the sequence; detecting, in the selected image, a small set of landmarks, corresponding to the face of the subject, using a convolutional neural network, trained to take as input an image region corresponding to the face of the—subject and to return a set of coordinates at computational speeds approximating real time; projectively fitting a three-dimensional character model to the detected landmarks, and using the fitted model to estimate physical locations of additional landmarks, so as to provide a complete hypothesized set of facial landmarks; and in a feature tracker process, updating the hypothesized set of facial landmarks to improve convergence between predicted feature locations and their actual physical locations based on data sampled from the selected image.

Abstract: Turbine assemblies and related turbocharger systems having direct turbine interfaces are provided. One exemplary turbine assembly includes a first turbine housing having an outlet portion defining a fluid outlet of a first turbine and a second turbine housing having an inlet portion defining a fluid inlet of a second turbine, wherein at least a portion of the inlet portion radially surrounds at least a portion of the outlet portion to provide a direct interface from the fluid outlet of the first turbine to the fluid inlet of the second turbine in an axial direction.

Abstract: Turbine assemblies and related turbocharger systems having direct turbine interfaces are provided. One exemplary turbine assembly includes a first turbine housing having an outlet portion defining a fluid outlet of a first turbine and a second turbine housing having an inlet portion defining a fluid inlet of a second turbine, wherein at least a portion of the inlet portion radially surrounds at least a portion of the outlet portion to provide a direct interface from the fluid outlet of the first turbine to the fluid inlet of the second turbine in an axial direction.

Abstract: A variable geometry turbocharger assembly comprises a unison ring which rotates about a longitudinal axis defined by a shaft on which a turbine wheel and compressor wheel are attached in order to pivot a plurality of vanes and thereby control a flow of exhaust gas to the turbine wheel. The unison ring rotates on at least one guide pin which is secured to the turbocharger. The guide pins may be press-fit into apertures in a center housing at an outer pilot surface. In other embodiments the guide pins may be press-fit into a different part of the turbocharger such as a nozzle ring. The guide pins may each have a wear surface which defines a circumferential radius of curvature which is substantially equal to a circumferential radius of curvature of a radially inner surface of the unison ring.

Abstract: A variable geometry turbocharger assembly comprises a unison ring which rotates about a longitudinal axis defined by a shaft on which a turbine wheel and compressor wheel are attached in order to pivot a plurality of vanes and thereby control a flow of exhaust gas to the turbine wheel. The unison ring rotates on at least one guide pin which is secured to the turbocharger. The guide pins may be press-fit into apertures in a center housing at an outer pilot surface. In other embodiments the guide pins may be press-fit into a different part of the turbocharger such as a nozzle ring. The guide pins may each have a wear surface which defines a circumferential radius of curvature which is substantially equal to a circumferential radius of curvature of a radially inner surface of the unison ring.