Abstract: The embodiments disclosed herein generally relate to identifying and removing background noise in spectroscopic imaging of a sample. Because white-light has essentially constant intensity at every wavelength, background noise caused by white light can be identified and removed from spectroscopic measurements including Raman spectroscopy. Thus, once the Raman spectrum for a sample is obtained, it may be corrected to remove the white-light dispersive spectrum in accordance with the embodiments disclosed herein.

Abstract: A fiberscope device is disclosed which is suitable for video imaging, laser Raman spectroscopy and laser Raman spectroscopic (i.e. chemical) imaging. The fiberscope design minimizes fiber background interference arising from the laser delivery fiber optic and the coherent fiber optic light gathering bundle while maintaining high light throughput efficiency through the use of integrated spectral filters. In the fiberscope design, the laser delivery fiber optic is offset from the coherent fiber optic light gathering bundle. The laser delivery field is captured entirely by the light gathering field of view of the coherent fiber bundle. The fiberscope incorporates spectral filter optical elements that provide environmental insensitivity, particularly to temperature and moisture.

Abstract: Pathogenic microorganisms are detected and classified by spectral imaging of the Raman light scattered by the organisms.

Abstract: Pathogenic microorganisms are detected in a wide field of view and classified by Raman light scattered light from these organisms together with digital pattern recognition of their spectral patterns.

Abstract: Apparatus and methods for spatially resolved Raman detection of molecules indicative of the borders of lesions with normal tissue are disclosed. A region of biological tissue was illuminated with monochromatic light. A Raman shifted light signal is detected from endogenous molecules in the region, the molecules being spatially organized in a localized first area of the region. These molecules are indicative of a border between normal tissue and a lesion. The Raman shifted light signal is then spatially resolved in at least one direction.

Abstract: Apparatus and methods for spatially resolved Raman detection of calcification in tissues are disclosed. A region of soft non-arterial biological tissue is illuminated with monochromatic light. A spatially organized first area of calcified tissue is then detected in the region by detecting a Raman shifted light signal. The Raman shifted light signal is spatially resolved in at least one direction.

Abstract: Pathogenic microorganisms are detected and classified by spectral imaging of the Raman light scattered by the organisms.

Abstract: Pathogenic microorganisms are detected and classified by spectral imaging of the Raman light scattered by the organisms.

Abstract: A fiberscope device is disclosed which is suitable for video imaging, laser Raman spectroscopy and laser Raman spectroscopic (i.e. chemical) imaging. The fiberscope design minimizes fiber background interference arising from the laser delivery fiber optic and the coherent fiber optic light gathering bundle while maintaining high light throughput efficiency through the use of integrated spectral filters. In the fiberscope design, the laser delivery fiber optic is offset from the coherent fiber optic light gathering bundle. The laser delivery field is captured entirely by the light gathering field of view of the coherent fiber bundle. The fiberscope incorporates spectral filter optical elements that provide environmental insensitivity, particularly to temperature and moisture.

Abstract: Pathogenic microorganisms are detected and classified by spectral imaging of the Raman light scattered by the organisms.

Abstract: An apparatus incorporated within a spectroscopic imaging system, typically a microscope, but also applicable to other image gathering platforms, namely fiberscopes, macrolens imaging systems and telescopes employing a polarizing beam splitting element is disclosed. The apparatus allows simultaneous spectroscopic (i.e. chemical) imaging and rapid acquisition spectroscopy to be performed without the need for moving mechanical parts or time sequenced sampling and without introducing significant optical signal loss or degradation to the spectroscopic imaging capability. In addition, the apparatus affords a more compact design, an improved angular field of view and an improved overall ruggedness of optical design at a lower manufacturing and maintenance cost compared to previous devices.

Abstract: Pathogenic microorganisms are detected and classified by spectral imaging of the Raman light scattered by the organisms.

Abstract: Apparatus and methods for spatially resolved Raman detection of calcification in tissues are disclosed. A region of soft non-arterial biological tissue is illuminated with monochromatic light. A spatially organized first area of calcified tissue is then detected in the region by detecting a Raman shifted light signal. The Raman shifted light signal is spatially resolved in at least one direction.

Abstract: Apparatus and methods for spatially resolved Raman detection of molecules indicative of the borders of lesions with normal tissue are disclosed. A region of biological tissue was illuminated with monochromatic light. A Raman shifted light signal is detected from endogenous molecules in the region, the molecules being spatially organized in a localized first area of the region. These molecules are indicative of a border between normal tissue and a lesion. The Raman shifted light signal is then spatially resolved in at least one direction.

Abstract: An apparatus incorporated within a spectroscopic imaging system, typically a microscope, but also applicable to other image gathering platforms, namely fiberscopes, macrolens imaging systems and telescopes employing a polarizing beam splitting element is disclosed. The apparatus allows simultaneous spectroscopic (i.e. chemical) imaging and rapid acquisition spectroscopy to be performed without the need for moving mechanical parts or time sequenced sampling and without introducing significant optical signal loss or degradation to the spectroscopic imaging capability. In addition, the apparatus affords a more compact design, an improved angular field of view and an improved overall ruggedness of optical design at a lower manufacturing and maintenance cost compared to previous devices.

Abstract: An optical analysis system includes an optical filter mechanism disposed to receive light from a light source and configured to optically compress data carried by the light into at least one orthogonal component of the light. A detector mechanism in operative communication with the optical filter mechanism measures a property of the at least one orthogonal component to measure the data.