Abstract: The invention provides a method for obtaining a broadly neutralizing antibody (bNab), including screening memory B cell cultures from a donor PBMC sample for neutralization activity against a plurality of HIV-1 species, cloning a memory B cell that exhibits broad neutralization activity; and rescuing a monoclonal antibody from that memory B cell culture. The resultant monoclonal antibodies may be characterized by their ability to selectively bind epitopes from the Env proteins in native or monomeric form, as well as to inhibit infection of HIV-1 species from a plurality of clades. Compositions containing human monoclonal anti-HIV antibodies used for prophylaxis, diagnosis and treatment of HIV infection are provided. Methods for generating such antibodies by immunization using epitopes from conserved regions within the variable loops of gp120 are provided. Immunogens for generating anti-HIV1 bNAbs are also provided. Furthermore, methods for vaccination using suitable epitopes are provided.

Abstract: Novel tools and techniques are provided for implementing network experience shifting, and, in particular embodiments, using either a roaming or portable hypervisor associated with a user or a local hypervisor unassociated with the user. In some embodiments, a network node in a first network might receive, via a first network access device in a second network, a request from a user device to establish roaming network access, and might authenticate a user associated with the user device, the user being unassociated with the first network access device. Based on a determination that the user is authorized to access data, content, profiles, and/or software applications that are accessible via a second network access device, the network node might establish a secure private connection through a hypervisor or container communicatively coupled to the first network access device to provide the user with access to her data, content, profiles, and/or software applications.

Abstract: An engine component seal assembly and method of sealing a coolant passage from an engine component are provided. The seal assembly includes a sealing sleeve sized and dimensioned to slip fit into an engine component mounting bore and a retaining ring sized and dimensioned to be axially inserted into the sleeve. The ring contacts the sleeve and applies a radial force sufficient to create an interference fit and to move or yield an interface portion of the sleeve radially outward into sealing abutment against a wall forming the mounting bore to create a secure and reliable annular fluid seal.

Abstract: An engine component seal assembly and method of sealing a coolant passage from an engine component are provided. The seal assembly includes a sealing sleeve sized and dimensioned to slip fit into an engine component mounting bore and a retaining ring sized and dimensioned to be axially inserted into the sleeve. The ring contacts the sleeve and applies a radial force sufficient to create an interference fit and to move or yield an interface portion of the sleeve radially outward into sealing abutment against a wall forming the mounting bore to create a secure and reliable annular fluid seal.

Abstract: An injector seal assembly and method of sealing a coolant passage from an injector are provided. The seal assembly includes a sealing sleeve sized and dimensioned to slip fit into an injector mounting bore and a retaining ring sized and dimensioned to be axially inserted into the sleeve. The ring contacts the sleeve and applies a radial force sufficient to create an interference fit and to move or yield an interface portion of the sleeve radially outward into sealing abutment against a wall forming the injector mounting bore to create a secure and reliable annular fluid seal.

Abstract: An injector seal assembly and method of sealing a coolant passage from an injector are provided. The seal assembly includes a sealing sleeve sized and dimensioned to slip fit into an injector mounting bore and a retaining ring sized and dimensioned to be axially inserted into the sleeve. The ring contacts the sleeve and applies a radial force sufficient to create an interference fit and to move or yield an interface portion of the sleeve radially outward into sealing abutment against a wall forming the injector mounting bore to create a secure and reliable annular fluid seal.

Abstract: A method and apparatus for pattern dyeing of textile substrates in which the dyeing is done in two stages. In the first stage, a dye is uniformly applied in a manner that allows the dye to fix on contact. The uniformly dyed substrate is then moved under a series of spaced arrays, each array being associated with a separate color and being comprised of a plurality of individually controlled liquid dye applicators. Liquid dye of a given color is selectively applied through one or more dye applicators as the substrate passes under the array associated with that color. The applied dye remains unfixed until the substrate passes under all the arrays and the patterning is complete, whereupon all dye applied by the dye applicators is fixed by steaming or other appropriate application of energy to the substrate.

Abstract: A liquid transfer device of disposable one-time use form to conduct a biochemical diagnostic or other assay comprises two liquid flow channels (10,110,210; 20,120,220) leading from a respective pair of channel ends to a common site (30,130,230) and operable to deliver liquid to this site in sequentially timed manner following simultaneous application of such liquid to the channel ends. Typically respectively different reagents (11,111,211; 21,121,221) are incorporated in the two channels, and a third reagent (31,131,231) at the common site, whereby a sample to be assayed in respect of a particular content can be applied to the site, immobilized by the third reagent, subsequently subjected to one channel reagent to detect any of the content of interest, and finally subjected to the other channel reagent to exhibit for the user any detected content.

Abstract: A liquid transfer device of disposable one-time use form to conduct a biochemical diagnostic or other assay comprises two liquid flow channels (10,110,210; 20,120,220) leading from a respective pair of channel ends to a common site (30,130,230) and operable to deliver liquid to this site in sequentially timed manner following simultaneous application of such liquid to the channel ends. Typically respectively different reagents (11,111,211; 21,121,221) are incorporated in the two channels, and a third reagent (31,131,231) at the common site, whereby a sample to be assayed in respect of a particular content can be applied to the site, immobilized by the third reagent, subsequently subjected to one channel reagent to detect any of the content of interest, and finally subjected to the other channel reagent to exhibit for the user any detected content.