Abstract: Disclosed herein are compositions and methods for isolating DNA, such as cell-free DNA (cfDNA). In some embodiments, the cell-free DNA is from a subject having or suspected of having cancer and/or the cell-free DNA comprises DNA produced by a tumor. In some embodiments, the DNA isolated by the method is captured using a sequence-variable target region set and an epigenetic target region set, wherein the sequence-variable target region set is captured with a greater capture yield than the epigenetic target region set. In some embodiments, captured cfDNA of the sequence-variable target region set is sequenced to a greater depth of sequencing than captured cfDNA of the epigenetic target region set.

Abstract: In an aspect, a method for evaluating the partitioning of nucleic acid molecules in a sample of polynucleotides based on epigenetic state, comprising: (a) adding a set of epigenetic-control nucleic acid molecules to the nucleic acid molecules in the sample of polynucleotides, whereby producing a spiked-in sample; (b) partitioning nucleic acid molecules of the spiked-in sample into plurality of partitioned sets; (c) enriching a subset of molecules from the plurality of partitioned sets to generate enriched molecules, wherein the enriched molecules comprises a group of epigenetic-control nucleic acid molecules and a group of nucleic acid molecules from the sample of polynucleotides; (d) sequencing the enriched molecules to produce sequencing reads; (e) analyzing the sequencing reads to generate one or more epigenetic partition scores of the epigenetic-control nucleic acid molecules; and (f) comparing the one or more epigenetic partition scores with one or more epigenetic partition cut-offs.

Abstract: An oil collection device for use with an engine having an oil filter mounting structure and an oil filter mounted thereto is provided. The oil collection device comprises a tray including a concavity that is open from above. The oil collection device further comprises a first connector formed on the tray, in which the first connector includes an attachment structure configured to removably attach to a portion of the engine below the oil filter. The oil collection device further comprises a second connector formed on the tray, in which the second connector has a drain hole formed therein, the drain hole extending through the tray and opening to the concavity, the second connector being configured to attach to a drain hose.

Abstract: Systems and methods for targeted advertising to specific consumers are disclosed. A system may include a memory storing instructions; and at least one processor configured to execute the instructions to: receive, over a network, consumer data from a client device; identify a plurality of client-provided consumers from the consumer data; obtain a plurality of unique consumer identifiers corresponding to the plurality of client-provided consumers; and identify at least one first overlapping unique consumer identifier by matching at least one of the plurality of client-provided consumers with at least one publisher-provided consumer provided by a first publisher device of a plurality of publisher devices, the first publisher device having a highest priority among the plurality of publisher devices.

Abstract: A central routing function (CRF) comprises back-to-back user agent application that receives a request for a call route list from a network element, wherein the request comprises a destination telephone number, a list generation application that obtains the call route list from the prioritized call route lists stored in the non-transitory memory based on the destination telephone number, wherein the call route list comprises a plurality of addresses, a pseudo domain name service (DNS) application that translates each address in the call route list into an Internet Protocol (IP) address, in which the back-to-back user agent application attempts to terminate a call using the IP address in the call route list, and in which the CRF platform is implemented in a software container.

Abstract: Disclosed herein are compositions and methods for isolating DNA, such as cell-free DNA (cfDNA). In some embodiments, the cell-free DNA is from a subject having or suspected of having cancer and/or the cell-free DNA comprises DNA produced by a tumor. In some embodiments, the DNA isolated by the method is captured using a sequence-variable target region set and an epigenetic target region set, wherein the sequence-variable target region set is captured with a greater capture yield than the epigenetic target region set. In some embodiments, captured cfDNA of the sequence-variable target region set is sequenced to a greater depth of sequencing than captured cfDNA of the epigenetic target region set.

Abstract: Systems and methods for targeted advertising to specific consumers are disclosed. A system may include a memory storing instructions; and at least one processor configured to execute the instructions to: receive, over a network, consumer data from a client device; identify a plurality of client-provided consumers from the consumer data; obtain a plurality of unique consumer identifiers corresponding to the plurality of client-provided consumers; and identify at least one first overlapping unique consumer identifier by matching at least one of the plurality of client-provided consumers with at least one publisher-provided consumer provided by a first publisher device of a plurality of publisher devices, the first publisher device having a highest priority among the plurality of publisher devices.

Abstract: Disclosed herein are compositions and methods for isolating DNA, such as cell-free DNA (cfDNA). In some embodiments, the cell-free DNA is from a subject having or suspected of having cancer and/or the cell-free DNA comprises DNA produced by a tumor. In some embodiments, the DNA isolated by the method is captured using a sequence-variable target region set and an epigenetic target region set, wherein the sequence-variable target region set is captured with a greater capture yield than the epigenetic target region set. In some embodiments, captured cfDNA of the sequence-variable target region set is sequenced to a greater depth of sequencing than captured cfDNA of the epigenetic target region set.

Abstract: Systems and methods for targeted advertising to specific consumers are disclosed. A system may include a memory storing instructions; and at least one processor configured to execute the instructions to: receive, over a network, consumer data from a client device; identify a plurality of client-provided consumers from the consumer data; obtain a plurality of unique consumer identifiers corresponding to the plurality of client-provided consumers; and identify at least one first overlapping unique consumer identifier by matching at least one of the plurality of client-provided consumers with at least one publisher-provided consumer provided by a first publisher device of a plurality of publisher devices, the first publisher device having a highest priority among the plurality of publisher devices.

Abstract: Disclosed herein are compositions and methods for isolating DNA, such as cell-free DNA (cfDNA). In some embodiments, the cell-free DNA is from a subject having or suspected of having cancer and/or the cell-free DNA comprises DNA produced by a tumor. In some embodiments, the DNA isolated by the method is captured using a sequence-variable target region set and an epigenetic target region set, wherein the sequence-variable target region set is captured with a greater capture yield than the epigenetic target region set. In some embodiments, captured cfDNA of the sequence-variable target region set is sequenced to a greater depth of sequencing than captured cfDNA of the epigenetic target region set.

Abstract: In an aspect, a method for evaluating the partitioning of nucleic acid molecules in a sample of polynucleotides based on epigenetic state, comprising: (a) adding a set of epigenetic-control nucleic acid molecules to the nucleic acid molecules in the sample of polynucleotides, whereby producing a spiked-in sample; (b) partitioning nucleic acid molecules of the spiked-in sample into plurality of partitioned sets; (c) enriching a subset of molecules from the plurality of partitioned sets to generate enriched molecules, wherein the enriched molecules comprises a group of epigenetic-control nucleic acid molecules and a group of nucleic acid molecules from the sample of polynucleotides; (d) sequencing the enriched molecules to produce sequencing reads; (e) analyzing the sequencing reads to generate one or more epigenetic partition scores of the epigenetic-control nucleic acid molecules; and (f) comparing the one or more epigenetic partition scores with one or more epigenetic partition cut-offs.

Abstract: The disclosure provides methods for processing nucleic acid populations containing different forms (e.g., RNA and DNA, single-stranded or double-stranded) and/or extents of modification (e.g., cytosine methylation, association with proteins). These methods accommodate multiple forms and/or modifications of nucleic acid in a sample, such that sequence information can be obtained for multiple forms. The methods also preserve the identity of multiple forms or modified states through processing and analysis, such that analysis of sequence can be combined with epigenetic analysis.

Abstract: A selectable detent assembly that has a housing with a plurality of passageways. Within the housing is a selector spool that is disposed within a selector passageway and is rotatable to provide a plurality of rotational positions including a no detent position, a kick down position, and a continuous detent position. A pilot check valve is disposed within the housing and provides a fluid flow path from a pilot passageway to the selector passageway and a detent passageway. A detent arming piston is disposed within the detent passageway and is connected to a directional control valve spool. Thus, depending on the rotational position of the selector spool, the selector spool can either provide a continuous flow path through the selector passageway to a tank, a flow path that is controlled by the axial position of the selector spool or prevent flow to the tank in order to operate the detent arming piston.

Abstract: A knowledge Management (KM) tool that allows persons in an organization to more rapidly locate the people and information they need to answer their questions. Identification and visualization of information aggregate founders, including category creators, helps in locating expertise, and additionally helps to reveal and magnify hidden knowledge dynamics within the organization.

Abstract: A selectable detent assembly that has a housing with a plurality of passageways. Within the housing is a selector spool that is disposed within a selector passageway and is rotatable to provide a plurality of rotational positions including a no detent position, a kick down position, and a continuous detent position. A pilot check valve is disposed within the housing and provides a fluid flow path from a pilot passageway to the selector passageway and a detent passageway. A detent arming piston is disposed within the detent passageway and is connected to a directional control valve spool. Thus, depending on the rotational position of the selector spool, the selector spool can either provide a continuous flow path through the selector passageway to a tank, a flow path that is controlled by the axial position of the selector spool or prevent flow to the tank in order to operate the detent arming piston.

Abstract: A system and method for evaluating an information aggregate. A metrics database stores document indicia including document attributes and associated person. A query engine responsive to a user request and the metrics database aggregates documents having same, unique attributes in a plurality of information aggregates, and determines the intersections of said information aggregates. A visualization engine displays these intersections selectively with reference to people, documents, usage patterns or categories.

Abstract: A system and method for determining a social network. A metrics database stores virtual object indicia including document attributes, activities, and associated persons. A query engine responsive to a user request and the metrics database infers individual connections to the virtual object by individuals executing the activities. A visualization engine visualizes membership in the social network as including these individuals and the activities connecting them. The query engine further collects a plurality of documents having non-unique values on a first shared attribute into an aggregate, and the visualization engine further filters the social network to include only individuals having membership in the aggregate.

Abstract: System and method for evaluating an information aggregate. A metrics database stores document indicia including document attributes, associated persons and age of creation. A query engine responsive to a user request and the metrics database aggregates documents having same, unique attributes and calculates therefrom, or from meta data defining the aggregate, an age value of the aggregate. A visualization engine visualizes age values for a plurality of aggregates at a client display.

Abstract: System and method for evaluating an information aggregate. A metrics database stores document indicia including document attributes, associated persons and usage metrics. A query engine collects a plurality of documents having non-unique values on a shared attribute into an information aggregate and tracks usage metrics with respect to the documents. A visualization engine visualizes the usage metrics with respect to a single aggregate, or as a composite or comparison of usage metrics for a plurality of aggregates.

Abstract: Information in a database collection of knowledge resources is evaluated by collecting a plurality of documents having non-unique values on a shared attribute into an information aggregate; assigning to each document an usefulness value; and calculating and visualizing the acceleration of the aggregate as a ratio of a sum of the usefulness values for documents recently included in the information aggregate to a sum of the usefulness values for all documents in the aggregate.