Abstract: Disclosed herein are compositions and methods for detecting RNA binding sites and RNA interacting partners involving the use of a modified capture oligonucleotide having a dual toehold design.

Abstract: The invention provides methods and compositions for generating mutations in new nucleic acid molecules through incorporation of a transformable nucleoside into the nucleic acid and subsequent transformation of the nucleoside through oxidative-nucleophilic-aromatic-substitution chemistry, referred to as TimeLapse chemistry. The invention further provides methods for detecting the mutations, referred to as TimeLapse-seq.

Abstract: Disclosed herein are compositions and methods for detecting RNA binding sites and RNA interacting partners involving the use of a modified capture oligonucleotide having a dual toehold design.

Abstract: Methods and assays are provided for isolating factors including polypeptides, ribonucleic acids (RNAs) and polypeptide complexes that are associated with a target nucleic acid sequence. The target nucleic acid sequence may be comprised within chromatin. The methods are suitable for identification and characterisation of factors including non-coding RNAs (ncRNAs) that associate with specified genomic loci.

Abstract: The invention provides methods and compositions for generating mutations in new nucleic acid molecules through incorporation of a transformable nucleoside into the nucleic acid and subsequent transformation of the nucleoside through oxidative-nucleophilic-aromatic-substitution chemistry, referred to as TimeLapse chemistry. The invention further provides methods for detecting the mutations, referred to as TimeLapse-seq.

Abstract: Methods and assays are provided for isolating factors including polypeptides, ribonucleic acids (RNAs) and polypeptide complexes that are associated with a target nucleic acid sequence. The target nucleic acid sequence may be comprised within chromatin. The methods are suitable for identification and characterisation of factors including non-coding RNAs (ncRNAs) that associate with specified genomic loci.

Abstract: A motor vehicle 10 includes a hydromechanical powersplit transmission 11, a prime mover 13, an engine drive shaft 14, drive wheels 15, a differential 16, a differential drive shaft 17, and frame rails 18. The transmission 11 includes a connecting plate 25 having a hydraulic side 25a with a pressurized hydraulic chamber 36 filled with hydraulic fluid and a gear set side 25b with an atmospheric pressure gear side chamber 37 partially filled with lubricating oil. The connecting plate 25 provides a hydraulic manifold. A prime mover input shaft 41, 62 extends through the hydraulic chamber 36 and connecting plate 25 and is drivingly connected to one of the planetary gear components 71. Hydraulic pump motor units 42, 43 include hydraulic pump motor unit drive shafts 44, 45 that extend through connecting plate 25 and are drivingly connected to planetary gear components 71.

Abstract: Methods for isolating non-coding nucleic acids that are associated with chromatin at a target genomic locus are provided.

Abstract: A location database is maintained with information regarding each of a number of different geographical items in different locations. A user database is also maintained with user records for various users, each user record comprising the user's identification data. In addition, in at least one of the location database and the user database, an identification is maintained of different geographical items which each user has visited. Geographical-item-to-geographical-item similarities are defined between different geographical items based on the user database. User-to-user similarities between the users are defined based on the user database. A weighted combination of the defined geographical-item-to-geographical-item and user-to-user similarities is computed and particularly relevant geographical items in a given location are identified to a user based on the weighted combination.

Abstract: In a variable displacement type hydraulic pump/motor assembly including at least one internally movable component, such as a yoke, a swashplate, and the means for moving the swashplate, the improvement includes imparting on this component absolute location indicia markings and the addition of an optical/electronic sensor unit capable of reading such indicia markings fixedly located on a housing of the pump/motor assembly and including an optical read head extending into the housing interior and located at a spaced distance from the indicia markings for reading same, relative to a reference location, and producing a signal indicative of the absolute position of the movable component. The signal can then, in turn, be used for controlling the displacement of the pump/motor assembly. A method for determining the absolute component position is also set forth.

Abstract: Improvement, in hydraulic systems, such as for vehicle transmissions and the like, in the form of a hydraulic reservoir with integrated heat exchanger having fluid supply/return and fluid return ports, including a central section, having inner and outer shell portions with a first gap therebetween, the outer shell being provided with a plurality of radially-spaced, external cooling fins, longitudinally-directed for the length of the central section; the ends of the latter being closed off via respective bottom and top cap portions, thereby defining a central fluid cavity, the top cap portion having spaced outer and inner cap portions, with a second gap therebetween, with a fluid inlet port, in the outer cap portion, directing incoming fluid from the second gap into and through the entire length of the first gap before entering the central fluid cavity, thereby maximizing heat transfer from the fluid via the plurality of cooling fins.

Abstract: A Bulk Mail Container (BMC) unloader uses a simple crossed four bar-linkage to move a predetermined point on a BMC unloader in a substantially straight line while the overall BMC unloader rotates about that predetermined point so as to upend the BMC container. This takes advantage of the crossed four-bar's substantially straight-line motion over a limited range of its motion, as well as the rotational motion of the particular unloader link disclosed herein. This is advantageous for unloading. In use, a BMC container is simply rolled into the BMC unloader, and the entire combination of the BMC container and BMC unloader is then actuated to produce substantially straight line motion along a suitably selected “center” point of the BMC unloader while the overall combination rotates about the center point so as to upend the BMC for unloading.

Abstract: A Bulk Mail Container (BMC) unloader uses a simple crossed four bar-linkage to move a predetermined point on a BMC unloader in a substantially straight line while the overall BMC unloader rotates about that predetermined point so as to upend the BMC container. This takes advantage of the crossed four-bar's substantially straight-line motion over a limited range of its motion, as well as the rotational motion of the particular unloader link disclosed herein. This is advantageous for unloading. In use, a BMC container is simply rolled into the BMC unloader, and the entire combination of the BMC container and BMC unloader is then actuated to produce substantially straight line motion along a suitably selected “center” point of the BMC unloader while the overall combination rotates about the center point so as to upend the BMC for unloading.

Abstract: A Bulk Mail Container (BMC) unloader uses a simple crossed four bar-linkage to move a predetermined point on a BMC unloader in a substantially straight line while the overall BMC unloader rotates about that predetermined point so as to upend the BMC container. This takes advantage of the crossed four-bar's substantially straight-line motion over a limited range of its motion, as well as the rotational motion of the particular unloader link disclosed herein. This is advantageous for unloading. In use, a BMC container is simply rolled into the BMC unloader, and the entire combination of the BMC container and BMC unloader is then actuated to produce substantially straight line motion along a suitably selected “center” point of the BMC unloader while the overall combination rotates about the center point so as to upend the BMC for unloading.