Abstract: Described herein are fusion proteins along with compositions and systems comprising a fusion protein. Also described herein are methods of using and/or producing a fusion protein such as methods of using a fusion protein for modifying or editing a target nucleic acid.

Abstract: A system for detecting and warning a user of an object in a path of a vehicle includes a detection module configured to detect an object in a path of a vehicle, and a control module in communication with the detection module. The control module is configured to receive vehicle data from one or more vehicle sensors, determine a height of the detected object, determine a ground clearance of the vehicle based on the received vehicle data, and in response to the height of the detected object being greater than a threshold based on the ground clearance of the vehicle, generate a signal to notify the user of the detected object. Other examples systems and methods for detecting and warning a user of an object in a path of a vehicle are also disclosed.

Abstract: A golf vehicle including a golf bag support; wherein the golf vehicle is selected from a golf trolley and a golf cart; wherein the golf bag support includes a lower support member; wherein the lower support member includes one of an upwardly projecting locating member and a downwardly extending recess defined by a recess body portion; and wherein the upwardly projecting locating member or the recess body portion comprises a ferromagnetic material.

Abstract: A brace that includes a body and a sensor device disposed in the body, where the sensor device includes a sensor that is configured to measure at least one parameter associated with a position of the housing. The brace also includes a controller communicably coupled to the sensor device. The controller can be configured to receive a plurality of measurements of the at least one parameter taken by the sensor device. The controller can also be configured to evaluate each measurement of the plurality of measurements.

Abstract: The invention relates to peptide linkers and fusion proteins comprising linkers designed for optimizing the activity of the proteins comprised therein, and methods for using the same. The invention further relates to newly designed Cas12a-based adenine base editors.

Abstract: Described herein are engineered proteins and methods of use of such proteins. Also described herein are complexes, compositions, and systems including engineered proteins of the present invention, each of which may be used for modifying and/or editing a target nucleic acid. An engineered protein of the present invention may be an enzyme and/or may be an RNA-guided DNA-binding protein.

Abstract: Described herein are polypeptides and methods of use of such polypeptides. Also described herein are complexes, compositions, and systems including polypeptides of the present invention, each of which may be used for modifying and/or editing a target nucleic acid. A polypeptide of the present invention may be an enzyme that generates DNA (e.g., cDNA) from RNA.

Abstract: This invention relates to recombinant nucleic constructs comprising Type V CRISPR-Cas effector proteins, reverse transcriptases and extended guide nucleic acids and methods of use thereof for modifying nucleic acids in plants.

Abstract: This invention relates to recombinant nucleic constructs comprising Type V CRISPR-Cas effector proteins, reverse transcriptases and extended guide nucleic acids and methods of use thereof for modifying nucleic acids in plants.

Abstract: This invention relates to recombinant nucleic constructs comprising Type V CRISPR-Cas effector proteins, reverse transcriptases and extended guide nucleic acids and methods of use thereof for modifying nucleic acids in plants.

Abstract: This invention relates to variants of Cas12a nucleases having altered protospacer adjacent motif recognition specificity. The invention further relates to methods of making CRISPR-CAS nuclease variants and methods of modifying nucleic acids using the variants.

Abstract: This invention relates to variants of Cas12a nucleases having altered protospacer adjacent motif recognition specificity. The invention further relates to methods of making CRISPR-CAS nuclease variants and methods of modifying nucleic acids using the variants.

Abstract: The invention relates to peptide linkers and fusion proteins comprising linkers designed for optimizing the activity of the proteins comprised therein, and methods for using the same. The invention further relates to newly designed Cas12a-based adenine base editors.

Abstract: Described herein are engineered proteins and methods of use of such proteins. Also described herein are complexes, compositions, and systems including engineered proteins of the present invention, each of which may be used for modifying and/or editing a target nucleic acid. An engineered protein of the present invention may be an enzyme and/or may be an RNA-guided DNA-binding protein.

Abstract: This invention relates to compositions and methods for modifying an endogenous nucleic acid encoding a Phytochrome A (PHYA) polypeptide for suppression of the shade avoidance response in plants. The invention further relates to plants produced using the methods and compositions of the invention.

Abstract: The invention relates to peptide linkers and fusion proteins comprising linkers designed for optimizing the activity of the proteins comprised therein, and methods for using the same. The invention further relates to newly designed Cas12a-based adenine base editors.

Abstract: The invention relates to peptide linkers and fusion proteins comprising linkers designed for optimizing the activity of the proteins comprised therein, and methods for using the same. The invention further relates to newly designed Cas12a-based cytosine base editors.

Abstract: This invention relates to CRISPR-Cas nucleases codon optimized for expression in plants and nucleic acid constructs encoding base editors comprising a CRISPR-Cas nuclease and a deaminase domain, wherein the nucleic acid constructs are optimized for expression in a plant. The invention further relates to methods of modifying nucleic acids using the nucleic acid constructs.

Abstract: This invention relates to CRISPR-Cas nucleases codon optimized for expression in plants and nucleic acid constructs encoding base editors comprising a CRISPR-Cas nuclease and a deaminase domain, wherein the nucleic acid constructs are optimized for expression in a plant. The invention further relates to methods of modifying nucleic acids using the nucleic acid constructs.

Abstract: This invention relates to CRISPR-Cas nucleases codon optimized for expression in plants and nucleic acid constructs encoding base editors comprising a CRISPR-Cas nuclease and a deaminase domain, wherein the nucleic acid constructs are optimized for expression in a plant. The invention further relates to methods of modifying nucleic acids using the nucleic acid constructs.