Abstract: A procedure and a device for the production of vascular systems, with cell cultures being applied to support structures, uses a liquid to perform a pressing. The liquid used is a nutrient medium adjusted to the cell culture at a tuned atmosphere; a culture support membrane is put on a negative mould; press needles are pressed into this layer. Subsequently, a preliminary pressing is performed using a positive mould; after this, two negative moulds with corresponding positive mould are applied and the positive moulds are removed. The press needles are removed from one mould; both negative moulds are joined to one another. The press needles are arrested in the mould; press core needles located within the press needles are removed. A circulation pump system is connected to the press and flush needles and liquid is pumped through these.