Patents by Inventor Matthieu Stettler
Matthieu Stettler has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11254964Abstract: The present invention relates to a method of maintaining cell viability at a level of at least 80% for up to 14 days in a fed-batch culture of cells producing a recombinant protein, the method comprising maintaining the cell culture medium at a pH of 6.5 to 7.0 and at a temperature of 31° C. to 35° C. for at least 5 consecutive days of the duration of the cell culture method.Type: GrantFiled: March 13, 2017Date of Patent: February 22, 2022Assignee: FRESENIUS KABI DEUTSCHLAND GMBHInventors: Matthieu Stettler, Hervé Broly, Thomas Solacroup, Yolande Rouiller
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Patent number: 11186858Abstract: The present invention relates to a method of increasing biosimilarity of a TNF ? binding protein to a reference TNF ? binding protein by modifying the charge profile of the binding protein produced by cell culture in a cell culture medium, by making an adjustment to a cell culture condition The adjustment is an increase in the concentration of a metal ion in the cell culture medium and the adjustment occurs on one or more days during the cell culture method, whereby the adjustment in the cell culture condition results in the modification of the charge profile of the binding protein. The present invention also relates to a biosimilar protein obtainable by such a process.Type: GrantFiled: August 6, 2019Date of Patent: November 30, 2021Assignee: FRESENIUS KABI DEUTSCHLAND GMBHInventors: Thomas Solacroup, Matthieu Stettler, Martin Jordan, Hervé Broly
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Publication number: 20210147532Abstract: The present invention relates to a method for controlling the afucosylation level of a glycoprotein composition. The method comprises the control of the afucosylation level by selecting the appropriate temperature and/or pH. The invention also relates to glycoprotein compositions produced according to the method of the invention.Type: ApplicationFiled: May 23, 2019Publication date: May 20, 2021Inventors: MARTIN JORDAN, HERVÉ BROLY, MATTHIEU STETTLER, ELODIE CHARBAUT TALAND
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Patent number: 10767160Abstract: The invention is in the field of cell culture. Particularly the invention relates to methods of culturing a host cell expressing a recombinant protein in a cell culture medium comprising an effective amount of a triazine dye or supplemented with an effective amount of a triazine dye, whereby production of said protein is increased relative to cells grown without said triazine dye or any other inducer.Type: GrantFiled: July 7, 2016Date of Patent: September 8, 2020Assignee: ARES TRADING S.A.Inventors: Anais Muhr, David Bruhlmann, Martin Jordan, Herve Broly, Matthieu Stettler
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Patent number: 10745663Abstract: The invention is in the field of cell culture. Particularly the invention relates to methods of culturing a host cell expressing a recombinant protein in a cell culture medium comprising an effective amount of 4,4?diisothiocyanostilbene-2,2?-disulfonic acid (DIDS) or supplemented with an effective amount of DIDS, whereby production of said protein is increased relative to cells grown without DIDS.Type: GrantFiled: July 7, 2016Date of Patent: August 18, 2020Assignee: ARES TRADING S.A.Inventors: Anais Muhr, David Bruhlmann, Martin Jordan, Herve Broly, Matthieu Stettler
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Patent number: 10526631Abstract: The present invention relates to a method of reducing serine for asparagine misincorporation in a protein produced by a culture of cells in a cell culture method, the method comprising the addition of asparagine and iron to the cell culture medium.Type: GrantFiled: March 13, 2017Date of Patent: January 7, 2020Assignee: ARES TRADING S.A.Inventors: Thomas Solacroup, Matthieu Stettler, Hervé Broly
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Patent number: 10519479Abstract: The present invention relates to a cell culture method for adjusting the proportion of G0, G1, G2, and high-mannose galactosylation variants in a population of a recombinant protein produced by a culture of cells in a cell culture medium by supplementing the cell culture medium with manganese at one or more days of the cell culture method duration.Type: GrantFiled: March 13, 2017Date of Patent: December 31, 2019Assignee: ARES TRADING S.A.Inventors: Thomas Solacroup, Matthieu Stettler, Martin Jordan, Hervé Broly
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Patent number: 10138467Abstract: The present invention relates to new serum- and protein-free culture media. These media are high performance culture media, which notably improve mammalian fed-batch cultures. The present invention also relates to methods for preparing and/or designing the medium, and methods of use thereof.Type: GrantFiled: April 3, 2014Date of Patent: November 27, 2018Assignee: ARES TRADING S.A.Inventors: Herve Broly, Matthieu Stettler, Martin Jordan, Arnaud Perilleux, Yolande Rouiller
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Publication number: 20180201897Abstract: The invention is in the field of cell culture. Particularly the invention relates to methods of culturing a host cell expressing a recombinant protein in a cell culture medium comprising an effective amount of a triazine dye or supplemented with an effective amount of a triazine dye, whereby production of said protein is increased relative to cells grown without said triazine dye or any other inducer.Type: ApplicationFiled: July 7, 2016Publication date: July 19, 2018Inventors: ANAIS MUHR, DAVID BRUHLMANN, MARTIN JORDAN, HERVE BROLY, MATTHIEU STETTLER
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Publication number: 20180201896Abstract: The invention is in the field of cell culture. Particularly the invention relates to methods of culturing a host cell expressing a recombinant protein in a cell culture medium comprising an effective amount of 4,4?-diisothiocyanostilbene-2,2?-disulfonic acid (DIDS) or supplemented with an effective amount of DIDS, whereby production of said protein is increased relative to cells grown without DIDS.Type: ApplicationFiled: July 7, 2016Publication date: July 19, 2018Inventors: ANAIS MUHR, DAVID BRUHLMANN, MARTIN JORDAN, HERVE BROLY, MATTHIEU STETTLER
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Publication number: 20160264940Abstract: The present invention relates to new serum- and protein-free culture media. These media are high performance culture media, which notably improve mammalian fed-batch cultures. The present invention also relates to methods for preparing and/or designing the medium, and methods of use thereof.Type: ApplicationFiled: April 3, 2014Publication date: September 15, 2016Inventors: HERVE BROLY, MATTHIEU STETTLER, MARTIN JORDAN, ARNAUD PERILLEUX, YOLANDE ROUILLER
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Patent number: 8107715Abstract: The invention relates to a device for detecting a sample in a longitudinal sample container (10), comprising a sample container holder (30) for holding the sample container in a housing. Said sample container holder comprises a side viewing window in the region of at least one longitudinal side of the sample container, and a front viewing window (35) in the region at least one front side of the sample container. Said device also comprises a first illumination arrangement for illuminating the sample container through the front viewing window, a second illumination arrangement for illuminating the sample container through the side viewing window, and an imaging photodetector for detecting a first image of the sample illuminated by means of the first illumination arrangement, and a second image of the sample illuminated by means of the second illumination arrangement. Said detection takes place through the side viewing window.Type: GrantFiled: April 24, 2007Date of Patent: January 31, 2012Assignee: Sartorius Stedim Biotech GmbHInventors: Reinhard Baumfalk, Oscar-Werner Reif, Florian Wurm, Maria de Jesus, Martin Jordan, Matthieu Stettler, Stefan Obermann
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Patent number: 8093015Abstract: The invention relates to a method for determining the viability of cells in cell cultures, said method comprising the steps of: coloring the sample with a dye which can penetrate into individual cells depending on the viability of the latter, determining the proportions of cells which are colored to differing extents. The invention is distinguished by virtue of the fact that a cell suspension of the colored cells is centrifuged as a sample in a sample vessel (10) until the solid content present in the sample has settled as a compressed cell cake (13), and, in order to determine the proportions, the volumes of differently colored sections (131, 132, 133) of the compressed cell cake (13) are determined as a measure of the proportions of cells of different viability.Type: GrantFiled: April 24, 2007Date of Patent: January 10, 2012Assignee: Sartorius Stedim Biotech GmbHInventors: Stefan Obermann, Reinhard Baumfalk, Oscar-Werner Reif, Florian Wurm, Maria de Jesus, Matthieu Stettler, Martin Jordan
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Publication number: 20090263848Abstract: The invention relates to a method for determining the viability of cells in cell cultures, said method comprising the steps of: colouring the sample with a dye which can penetrate into individual cells depending on the viability of the latter, determining the proportions of cells which are coloured to differing extents. The invention is distinguished by virtue of the fact that a cell suspension of the coloured cells is centrifuged as a sample in a sample vessel (10) until the solid content present in the sample has settled as a compressed cell cake (13), and, in order to determine the proportions, the volumes of differently coloured sections (131, 132, 133) of the compressed cell cake (13) are determined as a measure of the proportions of cells of different viability.Type: ApplicationFiled: April 24, 2007Publication date: October 22, 2009Inventors: Stefan Obermann, Reinhard Baumfalk, Oscar-Werner Reif, Florian Wurm, Maria De Jesus, Matthieu Stettler, Martin Jordan
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Publication number: 20090233334Abstract: The present invention provides a novel method for culturing cells as well as a novel method for producing a recombinant protein by culturing cells at large scale (up to 1,500 L nominal volume and 750 L working volume), whereby an inflated bag provides a sterile, disposable cultivation chamber. The inflated bag is partially filled with liquid cultivation media and cells, and placed into a containment vessel. The containment vessel is positioned onto an orbitally shaken platform. The orbital shaking moves the containment vessel and thus the bag and induces thereby motion to the liquid contained therein (“shake mixing”). This motion (caused by orbital shaking) induces a dynamic force field that ensures cell suspension, bulk mixing, and oxygen transfer from the liquid surface to the respiring cells without damaging shear or foam generation.Type: ApplicationFiled: March 11, 2008Publication date: September 17, 2009Applicant: EXCELLGENE SAInventors: Markus Hildinger, Florian Wurm, Matthieu Stettler, Maria DeJesus
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Publication number: 20090103772Abstract: The invention relates to a device for detecting a sample in a longitudinal sample container (10), comprising a sample container holder (30) for holding the sample container in a housing. Said sample container holder comprises a side viewing window in the region of at least one longitudinal side of the sample container, and a front viewing window (35) in the region at least one front side of the sample container. Said device also comprises a first illumination arrangement for illuminating the sample container through the front viewing window, a second illumination arrangement for illuminating the sample container through the side viewing window, and an imaging photodetector for detecting a first image of the sample illuminated by means of the first illumination arrangement, and a second image of the sample illuminated by means of the second illumination arrangement. Said detection takes place through the side viewing window.Type: ApplicationFiled: April 24, 2007Publication date: April 23, 2009Applicant: Sartorius Stedim Biotech GmbHInventors: Reinhard Baumfalk, Oscar-Werner Reif, Florian Wurm, Maria De Jesus, Martin Jordan, Matthieu Stettler, Stefan Obermann