Abstract: The present invention generally relates to the field of biopolymers. In one aspect. the invention is directed to a method of preparing lyophilized and reconstituted gellan gum compositions. In further aspects. the invention relates to lyophilized compositions comprising gellan gum, reconstituted gellan gum compositions. dosage-unit formulations comprising lyophilized gellan gum, and their use in medicine or other sterile applications.

Abstract: The present solution includes a 3D printer designed to meet clean room requirements. The printer can be configured for 3D bioprinting. The printer can be used to create cellular scaffolds, tissue grafts, and organs. The printer can include a sterile (or clean room-like) environment for the printing of items. The printer can include an enclosure that isolates the manufacturing processes from the external environment and that can be sterilized between printing runs. The solution also includes a printing kit that can be independently sterilized and passed into the system's enclosure.