Patents by Inventor Matti Siika-Aho

Matti Siika-Aho has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20210388398
    Abstract: The present invention is directed to cellulytic host cells. The host cells of the invention expressing heterologous cellulases and are able to produce ethanol from cellulose. According to the invention, host cells expressing a combination of heterologous cellulases can be used to produce ethanol from cellulose. In addition, multiple host cells expressing different heterologous cellulases can be co-cultured together and used to produce ethanol from cellulose. Furthermore, the invention demonstrates for the first time the ability of Kluyveromyces to produce ethanol from cellulose. The yeast strains and co-cultures of yeast strains of the invention can be used to produce ethanol on their own, or can also be used in combination with externally added cellulases to increase the efficiency of saccharification and fermentation processes.
    Type: Application
    Filed: December 22, 2020
    Publication date: December 16, 2021
    Inventors: John McBride, Elena Brevnova, Mark Mellon, Allan Froehlich, Kristen Deleault, Vineet Rajgarhia, Riaan Den Haan, Merja Penttila, Marja Ilmen, Matti Siika-Aho, Jaana Uusitalo, Emily A. Stonehouse, Alan Gilbert, Haowen Xu, Deidre Willies, John Bardsley, Anu Koivula, Sanni Voutilainen
  • Patent number: 10266610
    Abstract: The present invention relates to a method of treating biomass, comprising providing a lignocellulosic biomass feedstock; contacting the biomass feedstock in a mixture, which is formed by the biomass, water and an alkaline agent, with an oxidizing agent at an elevated temperature; and continuing the contacting of the biomass feedstock with the oxidizing agent until a notable part of the lignin is solubilized. The step of providing the biomass feedstock comprises contacting a biomass raw-material containing cellulose, hemicellulose and lignin in an aqueous phase with an alkaline agent; continuing the contacting of the biomass with the alkaline agent until a significant portion of the hemicellulose is dissolved in the aqueous phase to provide a modified biomass; recovering the modified biomass; optionally opening the structure of the modified biomass by mechanical treatment; and using the modified biomass as a lignocellulosic biomass feedstock.
    Type: Grant
    Filed: October 19, 2015
    Date of Patent: April 23, 2019
    Assignee: TEKNOLOGIAN TUTKIMUSKESKUS VTT OY
    Inventors: Antero Varhimo, Matti Siika-Aho, Jari Sirviö, Sari Liukkonen, Marjo Määttänen
  • Publication number: 20180258449
    Abstract: The present invention is directed to cellulytic host cells. The host cells of the invention expressing heterologous cellulases and are able to produce ethanol from cellulose. According to the invention, host cells expressing a combination of heterologous cellulases can be used to produce ethanol from cellulose. In addition, multiple host cells expressing different heterologous cellulases can be co-cultured together and used to produce ethanol from cellulose. Furthermore, the invention demonstrates for the first time the ability of Kluyveromyces to produce ethanol from cellulose. The yeast strains and co-cultures of yeast strains of the invention can be used to produce ethanol on their own, or can also be used in combination with externally added cellulases to increase the efficiency of saccharification and fermentation processes.
    Type: Application
    Filed: May 21, 2018
    Publication date: September 13, 2018
    Inventors: John McBride, Elena Brevnova, Mark Mellon, Allan Froehlich, Kristen Deleault, Vineet Rajgarhia, Riaan Den Haan, Merja Penttila, Marja Ilmen, Matti Siika-Aho, Jaana Uusitalo, Emily A. Stonehouse, Alan Gilbert, Haowen Xu, Deidre Willies, John Bardsley, Anu Koivula, Sanni Voutilainen
  • Patent number: 9988652
    Abstract: The present invention is directed to cellulytic host cells. The host cells of the invention expressing heterologous cellulases and are able to produce ethanol from cellulose. According to the invention, host cells expressing a combination of heterologous cellulases can be used to produce ethanol from cellulose. In addition, multiple host cells expressing different heterologous cellulases can be co-cultured together and used to produce ethanol from cellulose. Furthermore, the invention demonstrates for the first time the ability of Kluveryomyces to produce ethanol from cellulose. The yeast strains and co-cultures of yeast strains of the invention can be used to produce ethanol on their own, or can also be used in combination with externally added cellulases to increase the efficiency of saccharification and fermentation processes.
    Type: Grant
    Filed: July 1, 2015
    Date of Patent: June 5, 2018
    Assignees: Lallemand Hungary Liquidity Management LLC, Stellenbosch University
    Inventors: John McBride, Elena Brevnova, Mark Mellon, Allan Froehlich, Kristen Deleault, Vineet Rajgarhia, Riaan Den Haan, Merja Penttila, Marja Ilmen, Matti Siika-Aho, Jaana Uusitalo, Emily A. Stonehouse, Alan Gilbert, Haowen Xu, Deidre Willes, John Bardsley, Anu Koivula, Sanni Voutilainen
  • Publication number: 20180044656
    Abstract: The present invention relates to the production of sugar hydrolysates from cellulosic material. The method may be used e.g. for producing fermentable sugars for the production of bioethanol from lignocellulosic material. Cellulolytic enzymes and their production by recombinant technology is described, as well as uses of the enzymes and enzyme preparations.
    Type: Application
    Filed: August 30, 2017
    Publication date: February 15, 2018
    Inventors: Jari Vehmaanpera, Marika Alapuranen, Terhi Puranen, Matti Siika-Aho, Jarno Kallio, Satu Jamsa, Sanni Voutilainen, Teemu Halonen, Liisa Viikari
  • Publication number: 20170298152
    Abstract: The present invention relates to a method of treating biomass, comprising providing a lignocellulosic biomass feedstock; contacting the biomass feedstock in a mixture, which is formed by the biomass, water and an alkaline agent, with an oxidizing agent at an elevated temperature; and continuing the contacting of the biomass feedstock with the oxidizing agent until a notable part of the lignin is solubilised. The step of providing the biomass feedstock comprises contacting a biomass raw-material containing cellulose, hemicellulose and lignin in an aqueous phase with an alkaline agent; continuing the contacting of the biomass with the alkaline agent until a significant portion of the hemicellulose is dissolved in the aqueous phase to provide a modified biomass; recovering the modified biomass; optionally opening the structure of the modified biomass by mechanical treatment; and using the modified biomass as a lignocellulosic biomass feedstock.
    Type: Application
    Filed: October 19, 2015
    Publication date: October 19, 2017
    Inventors: Antero Varhimo, Matti Siika-Aho, Jari Sirviö, Sari Liukkonen, Marjo Määttänen
  • Patent number: 9758777
    Abstract: The present invention relates to the production of sugar hydrolysates from cellulosic material. The method may be used e.g. for producing fermentable sugars for the production of bioethanol from lignocellulosic material. Cellulolytic enzymes and their production by recombinant technology is described, as well as uses of the enzymes and enzyme preparations.
    Type: Grant
    Filed: February 22, 2013
    Date of Patent: September 12, 2017
    Assignee: Roal OY
    Inventors: Jari Vehmaanperä, Marika Alapuranen, Terhi Puranen, Matti Siika-Aho, Jarno Kallio, Satu Hooman, Sanni Voutilainen, Teemu Halonen, Liisa Viikari
  • Patent number: 9593324
    Abstract: The present invention relates to the production of sugar hydrolysates from cellulosic material. The method may be used e.g. for producing fermentable sugars for the production of bioethanol from lignocellulosic material. Cellulolytic enzymes and their production by recombinant technology is described, as well as uses of the enzymes and enzyme preparations.
    Type: Grant
    Filed: October 3, 2013
    Date of Patent: March 14, 2017
    Assignee: Roal Oy
    Inventors: Jari Vehmaanperä, Marika Alapuranen, Terhi Puranen, Matti Siika-Aho, Jarno Kallio, Satu Jamsa, Sanni Voutilainen, Teemu Halonen, Liisa Viikari
  • Publication number: 20160010117
    Abstract: The present invention is directed to cellulytic host cells. The host cells of the invention expressing heterologous cellulases and are able to produce ethanol from cellulose. According to the invention, host cells expressing a combination of heterologous cellulases can be used to produce ethanol from cellulose. In addition, multiple host cells expressing different heterlogous cellulases can be co-cultured together and used to produce ethanol from cellulose. Furthermore, the invention demonstrates for the first time the ability of Kluveryomyces to produce ethanol from cellulose. The yeast strains and co-cultures of yeast strains of the invention can be used to produce ethanol on their own, or can also be used in combination with externally added cellulases to increase the efficiency of saccharification and fermentation processes.
    Type: Application
    Filed: July 1, 2015
    Publication date: January 14, 2016
    Inventors: John McBride, Elena Brevnova, Mark Mellon, Allan Froehlich, Kristen Deleault, Vineet Rajgarhia, Riaan Den Haan, Merja Penttila, Marja Ilmen, Matti Siika-Aho, Jaana Uusitalo, Emily A. Stonehouse, Alan Gilbert, Haowen Xu, Deidre Willes, John Bardsley, Anu Koivula, Sanni Voutilainen
  • Patent number: 9102955
    Abstract: Host cells, comprising Kluveryomyces expressing heterologous cellulases produce ethanol from cellulose In addition, multiple host cells expressing different heterlogous cellulases can be co-cultured together and used to produce ethanol from cellulose The recombinant yeast strains and co-cultures of the yeast strains can be used to produce ethanol on their own, or can also be used in combination with externally added cellulases to increase the efficiency of sacchanfication and fermentation processes.
    Type: Grant
    Filed: November 23, 2009
    Date of Patent: August 11, 2015
    Assignee: Lallemand Hungary Liquidity Management LLC
    Inventors: John McBride, Elena Brevnova, Mark Mellon, Allan Froehlich, Kristen Deleault, Vineet Rajgarhia, Riaan Den Haan, Merja Penttila, Marja Ilmen, Matti Siika-Aho, Jaana Uusitalo, Emily A. Stonehouse, Alan Gilbert, Haowen Xu, Deidre Willes, John Bardsley, Anu Koivula, Sanni Voutilainen
  • Publication number: 20140322762
    Abstract: The present invention relates to the production of sugar hydrolysates from cellulosic material. The method may be used e.g. for producing fermentable sugars for the production of bioethanol from lignocellulosic material. Cellulolytic enzymes and their production by recombinant technology is described, as well as uses of the enzymes and enzyme preparations.
    Type: Application
    Filed: October 3, 2013
    Publication date: October 30, 2014
    Applicant: ROAL OY
    Inventors: Jari VEHMAANPERÄ, Marika ALAPURANEN, Terhi PURANEN, Matti SIIKA-AHO, Jarno KALLIO, Satu HOOMAN, Sanni VOUTILAINEN, Teemu HALONEN, Liisa VIIKARI
  • Publication number: 20130327319
    Abstract: The present invention concerns a process for the mechanical or mechano-chemical treatment of biomass, wherein a mixture containing the biomass and optional further chemicals is pressed through the openings of one or more compactor one or more times. The process can be operated in a continuous manner by using more than one compactor and more than one compacting cycle, whereby the multiple compactors are operated sequentially.
    Type: Application
    Filed: February 23, 2012
    Publication date: December 12, 2013
    Applicant: TEKNOLOGIAN TUTKIMUSKESKUS VTT
    Inventors: Kirsi Immonen, Eino Sivonen, Kyosti Valta, Anne Kallioinen, Matti Siika-Aho
  • Publication number: 20130224801
    Abstract: The present invention relates to the production of sugar hydrolysates from cellulosic material. The method may be used e.g. for producing fermentable sugars for the production of bioethanol from lignocellulosic material. Cellulolytic enzymes and their production by recombinant technology is described, as well as uses of the enzymes and enzyme preparations.
    Type: Application
    Filed: February 22, 2013
    Publication date: August 29, 2013
    Applicant: ROAL OY
    Inventors: Jari VEHMAANPERÄ, Marika ALAPURANEN, Terhi PURANEN, Matti SIIKA-AHO, Jarno KALLIO, Satu HOOMAN, Sanni VOUTILAINEN, Teemu HALONEN, Liisa VIIKARI
  • Patent number: 8409836
    Abstract: The present invention relates to the production of sugar hydrolysates from cellulosic material. The method may be used e.g. for producing fermentable sugars for the production of bioethanol from lignocellulosic material. Cellulolytic enzymes and their production by recombinant technology is described, as well as uses of the enzymes and enzyme preparations.
    Type: Grant
    Filed: November 2, 2010
    Date of Patent: April 2, 2013
    Assignee: Roal Oy
    Inventors: Jari Vehmaanperä, Marika Alapuranen, Terhi Puranen, Matti Siika-Aho, Jarno Kallio, Satu Hooman, Sanni Voutilainen, Teemu Halonen, Liisa Viikari
  • Publication number: 20120129229
    Abstract: Host cells, comprising Kluveryomyces expressing heterologous cellulases produce ethanol from cellulose In addition, multiple host cells expressing different heterlogous cellulases can be co-cultured together and used to produce ethanol from cellulose The recombinant yeast strains and co-cultures of the yeast strains can be used to produce ethanol on their own, or can also be used in combination with externally added cellulases to increase the efficiency of sacchanfication and fermentation processes
    Type: Application
    Filed: November 23, 2009
    Publication date: May 24, 2012
    Inventors: John McBride, Elena Brevnova, Mark Mellon, Allan Froehlich, Kristen Deleault, Vineet Rajgarhia, Riaan Den Haan, Merja Penttila, Marja Ilmen, Matti Siika-Aho, Jaana Uusitalo, Emily A. Stonehouse, Alan Gilbert, Haowen Xu, Deidre Willes, John Bardsley, Anu Koivula, Sanni Voutilainen
  • Publication number: 20110045544
    Abstract: The present invention relates to the production of sugar hydrolysates from cellulosic material. The method may be used e.g. for producing fermentable sugars for the production of bioethanol from lignocellulosic material. Cellulolytic enzymes and their production by recombinant technology is described, as well as uses of the enzymes and enzyme preparations.
    Type: Application
    Filed: November 2, 2010
    Publication date: February 24, 2011
    Applicant: Roal OY
    Inventors: Jari Vehmaanperä, Marika Alapuranen, Terhi Puranen, Matti Siika-Aho, Jarno Kallio, Satu Hooman, Sanni Voutilainen, Teemu Halonen, Liisa Viikari
  • Patent number: 7754456
    Abstract: A process for producing ethanol from a fibrous lignocellulosic raw material. After pre-treatment of the raw material, the fibrous fraction is first hydrolysed at high consistency and then the modified material is subjected simultaneously to continued hydrolysis with a cellulase and to ethanol fermentation in a fermentation mixture. Fermentation is continued to convert an essential portion of the available cellulose into ethanol, then a liquid fraction containing solubilized hemicelluloses is added to the fermentation mixture and fermentation continued. By means of the invention, high fermentation rates, high ethanol concentrations and low ethanol production costs can be attained.
    Type: Grant
    Filed: June 6, 2005
    Date of Patent: July 13, 2010
    Assignee: Valtion Teknillinen Tutkimuskeskus
    Inventors: Merja Penttilä, Matti Siika-Aho, Jaana Uusitalo, Liisa Viikari
  • Publication number: 20090042266
    Abstract: The present invention relates to the production of sugar hydrolysates from cellulosic material. The method may be used e.g. for producing fermentable sugars for the production of bioethanol from lignocellulosic material. Cellulolytic enzymes and their production by recombinant technology is described, as well as uses of the enzymes and enzyme preparations.
    Type: Application
    Filed: June 19, 2008
    Publication date: February 12, 2009
    Applicant: Roal Oy
    Inventors: Jari VEHMAANPERA, Marika Alapuranen, Terhi Puranen, Matti Siika-Aho, Jarno Kallio, Satu Hooman, Sanni Voutilainen, Teemu Halonen, Liisa Viikari
  • Publication number: 20080044877
    Abstract: A process for producing ethanol from a fibrous lignocellulosic raw material. After pre-treatment of the raw material, the fibrous fraction is first hydrolysed at high consistency and then the modified material is subjected simultaneously to continued hydrolysis with a cellulase and to ethanol fermentation in a fermentation mixture. Fermentation is continued to convert an essential portion of the available cellulose into ethanol, then a liquid fraction containing solubilized hemicelluloses is added to the fermentation mixture and fermentation continued. By means of the invention, high fermentation rates, high ethanol concentrations and low ethanol production costs can be attained.
    Type: Application
    Filed: June 6, 2005
    Publication date: February 21, 2008
    Inventors: Merja Penttila, Matti Siika-Aho, Jaana Uusitalo, Liisa Viikari
  • Patent number: 7256032
    Abstract: The present invention relates to novel cellulase enzymes, especially novel endoglucanases including endoglucanase fusion proteins, preparations and compositions containing these endoglucanase enzymes and fusion proteins, expression vectors, host cells and methods for their preparation and uses of the cellulases, preparations and compositions in the textile, detergent and pulp and paper industries.
    Type: Grant
    Filed: December 22, 2005
    Date of Patent: August 14, 2007
    Assignee: AB Enzymes Oy
    Inventors: Leena Valtakari, Marika Alapuranen, Satu Hooman, Matti Siika-Aho, Jarno Kallio, Liisa Viikari, Pentti Ojapalo, Jari Vehmaanperä