Patents by Inventor Mattias Vangbo
Mattias Vangbo has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230152274Abstract: A system for performing capillary electrophoresis of multiple samples comprises a capillary containing a separation medium and having inlet and distal ends and an interrogation region; a power source configured to apply voltages between inlet and distal ends; and logic to cause execution of: applying a first substantially constant forward polarity electrophoresis voltage to the capillary; before all of the first DNA fragments have passed the interrogation region, applying a reverse polarity voltage pulse to the capillary, thereby transporting at least some of the first DNA fragments in the capillary toward the capillary inlet; introducing a second sample to the capillary inlet, the second sample comprising second DNA fragments having a plurality of different sizes; and applying a second substantially constant forward polarity electrophoresis voltage to the capillary to simultaneously perform electrophoresis on the second DNA fragments and the first DNA fragments.Type: ApplicationFiled: January 20, 2023Publication date: May 18, 2023Applicant: IntegenX, Inc.Inventors: Mattias Vangbo, David King, Arnaldo Barican
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Patent number: 11592421Abstract: Disclosed are methods for performing capillary electrophoresis on two or more nucleic acid samples. The methods employ a forward voltage to move a first sample forward from an inlet to an interrogation region in the capillary, then a backward voltage to move the first sample backward, and then a forward voltage again to move the first sample and a second sample forward. Systems and apparatuses for performing capillary electrophoresis are also provided.Type: GrantFiled: May 22, 2018Date of Patent: February 28, 2023Assignee: INTEGENX, INC.Inventors: Mattias Vangbo, David King, Arnaldo Barican
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Publication number: 20220051754Abstract: A method of testing a biological sample comprising deoxyribonucleic acid (DNA) molecules for presence of a plurality of alleles is described, wherein DNA fragments obtained using the biological sample and corresponding to different alleles have different fragment sizes. A capillary electrophoresis (CE) instrument is used to obtain test fragment sizing data for the biological sample. A pre-computed model is used to dynamically determine one or more synthetic allelic ladders, where the pre-computed model is derived via analysis of a plurality of fragment sizing data sets obtained from a plurality of previous allelic ladder sample runs conducted using CE instruments. The one or more synthetic or experimentally derived allelic ladders are used to find a sufficient fit to the test fragment sizing data to identify which of the plurality of alleles are present in the biological sample. The statistical analysis may comprise a principal component analysis including two principal components.Type: ApplicationFiled: August 13, 2021Publication date: February 17, 2022Applicant: Life Technologies CorporationInventor: Mattias Vangbo
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Publication number: 20220016632Abstract: Performing sample quantitation and sample amplification may be performed in a sample cartridge or sample cartridges. Sample quantitation using qPCR may be performed during STR PCR on the sample. Samples need not be normalized prior to performing STR PCR. In certain embodiments, qPCR and STR PCR are performed on the same cartridge, optionally at the same time (or in real-time, or overlapping in time) and optionally using some or all of the same PCR apparatus. In other embodiments, qPCR and STR PCR are performed on different cartridges. Quantitation of the STR PCR sample may be performed without substantially delaying the STR PCR process.Type: ApplicationFiled: December 4, 2019Publication date: January 20, 2022Inventors: Mattias Vangbo, David King
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Publication number: 20210003530Abstract: The present disclosure provides systems and methods for sample preparation, processing and analysis. Also provided in the present disclosure is a fully-integrated electrophoresis cartridge which has a small footprint and configured to removably engage with the system.Type: ApplicationFiled: May 11, 2020Publication date: January 7, 2021Inventors: Alexander Kindwall, Chungsoo Charles Park, Fedor IIkov, Stevan Jovanovich, David King, Tristan Robert Littlehale, David Grant Nilson, Rick Pittaro, Robert A. Schueren, Mattias Vangbo, Philip Justus Wunderle
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Patent number: 10690627Abstract: The present disclosure provides systems and methods for sample preparation, processing and analysis. Also provided in the present disclosure is a fully-integrated electrophoresis cartridge which has a small footprint and configured to removably engage with the system.Type: GrantFiled: October 21, 2015Date of Patent: June 23, 2020Assignee: IntegenX, Inc.Inventors: Alexander Kindwall, Chungsoo Charles Park, Fedor Ilkov, Stevan Jovanovich, David King, Tristan Robert Littlehale, David Grant Nilson, Rick Pittaro, Robert A. Schueren, Mattias Vangbo, Philip Justus Wunderle
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Publication number: 20200158685Abstract: Disclosed are methods for performing capillary electrophoresis on two or more nucleic acid samples. The methods employ a forward voltage to move a first sample forward from an inlet to an interrogation region in the capillary, then a backward voltage to move the first sample backward, and then a forward voltage again to move the first sample and a second sample forward. Systems and apparatuses for performing capillary electrophoresis are also provided.Type: ApplicationFiled: May 22, 2018Publication date: May 21, 2020Inventors: Mattias Vangbo, David King, Arnaldo Barican
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Patent number: 9663819Abstract: The invention provides a system that can process a raw biological sample, perform a biochemical reaction and provide an analysis readout. For example, the system can extract DNA from a swab, amplify STR loci from the DNA, and analyze the amplified loci and STR markers in the sample. The system integrates these functions by using microfluidic components to connect what can be macrofluidic functions. In one embodiment the system includes a sample purification module, a reaction module, a post-reaction clean-up module, a capillary electrophoresis module and a computer. In certain embodiments, the system includes a disposable cartridge for performing analyte capture. The cartridge can comprise a fluidic manifold having macrofluidic chambers mated with microfluidic chips that route the liquids between chambers. The system fits within an enclosure of no more than 10 ft3. and can be a closed, portable, and/or a battery operated system. The system can be used to go from raw sample to analysis in less than 4 hours.Type: GrantFiled: November 24, 2014Date of Patent: May 30, 2017Assignee: INTEGENX INC.Inventors: Stevan B. Jovanovich, William D. Nielsen, Mattias Vangbo, Ezra Van Gelder, Omar El-Sissi
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Patent number: 9341284Abstract: This invention provides a fluidic device comprising a diaphragm valve having a fluidics layer, an actuation layer and an elastic layer between the fluidics layer and the actuation layer, the elastic layer having a diaphragm that is mechanically sealed against the fluidics layer and the actuation layer by a sealing ring in the actuation layer.Type: GrantFiled: June 30, 2014Date of Patent: May 17, 2016Assignee: IntegenX Inc.Inventor: Mattias Vangbo
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Publication number: 20160116439Abstract: The present disclosure provides systems and methods for sample preparation, processing and analysis. Also provided in the present disclosure is a fully-integrated electrophoresis cartridge which has a small footprint and configured to removably engage with the system.Type: ApplicationFiled: October 21, 2015Publication date: April 28, 2016Inventors: Alexander Kindwall, Chungsoo Charles Park, Fedor Ilkov, Stevan Jovanovich, David King, Tristan Robert Littlehale, David Grant Nilson, Rick Pittaro, Robert A. Schueren, Mattias Vangbo, Philip Justus Wunderle
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Publication number: 20160016140Abstract: The invention provides systems, devices, methods, and kits for performing an integrated analysis. The integrated analysis can include sample processing, library construction, amplification, and sequencing. The integrated analysis can be performed within one or more modules that are fluidically connected to each other. The one or more modules can be controlled and/or automated by a computer. The integrated analysis can be performed on a tissue sample, a clinical sample, or an environmental sample. The integrated analysis system can have a compact format and return results within a designated period of time.Type: ApplicationFiled: July 21, 2015Publication date: January 21, 2016Applicant: INTEGENX INC.Inventors: Stevan B. Jovanovich, Seth Stern, Ezra Van Gelder, Mattias Vangbo
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Publication number: 20150136602Abstract: The invention provides a system that can process a raw biological sample, perform a biochemical reaction and provide an analysis readout. For example, the system can extract DNA from a swab, amplify STR loci from the DNA, and analyze the amplified loci and STR markers in the sample. The system integrates these functions by using microfluidic components to connect what can be macrofluidic functions. In one embodiment the system includes a sample purification module, a reaction module, a post-reaction clean-up module, a capillary electrophoresis module and a computer. In certain embodiments, the system includes a disposable cartridge for performing analyte capture. The cartridge can comprise a fluidic manifold having macrofluidic chambers mated with microfluidic chips that route the liquids between chambers. The system fits within an enclosure of no more than 10 ft3. and can be a closed, portable, and/or a battery operated system. The system can be used to go from raw sample to analysis in less than 4 hours.Type: ApplicationFiled: November 24, 2014Publication date: May 21, 2015Inventors: Stevan B. Jovanovich, William D. Nielsen, David S. Cohen, Michael Recknor, Mattias Vangbo, Ezra Van Gelder, Lars Majlof, Omar El-Sissi
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Patent number: 9012236Abstract: The invention provides a system that can process a raw biological sample, perform a biochemical reaction and provide an analysis readout. For example, the system can extract DNA from a swab, amplify STR loci from the DNA, and analyze the amplified loci and STR markers in the sample. The system integrates these functions by using microfluidic components to connect what can be macrofluidic functions. In one embodiment the system includes a sample purification module, a reaction module, a post-reaction clean-up module, a capillary electrophoresis module and a computer. In certain embodiments, the system includes a disposable cartridge for performing analyte capture. The cartridge can comprise a fluidic manifold having macrofluidic chambers mated with microfluidic chips that route the liquids between chambers. The system fits within an enclosure of no more than 10 ft3. and can be a closed, portable, and/or a battery operated system. The system can be used to go from raw sample to analysis in less than 4 hours.Type: GrantFiled: August 15, 2013Date of Patent: April 21, 2015Assignee: IntegenX Inc.Inventors: Stevan B. Jovanovich, William D. Nielsen, David S. Cohen, Michael Recknor, Mattias Vangbo, Ezra Van Gelder, Omar El-Sissi
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Publication number: 20150021502Abstract: This invention provides a fluidic device comprising a diaphragm valve having a fluidics layer, an actuation layer and an elastic layer between the fluidics layer and the actuation layer, the elastic layer having a diaphragm that is mechanically sealed against the fluidics layer and the actuation layer by a sealing ring in the actuation layer.Type: ApplicationFiled: June 30, 2014Publication date: January 22, 2015Inventor: Mattias Vangbo
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Publication number: 20140370519Abstract: The invention provides for devices and methods for interfacing microchips to cartridges and pneumatic manifolds. The cartridges, microchips, and pneumatic manifolds can be integrated with downstream preparation devices, such as thermal regulating devices and separation and analysis devices.Type: ApplicationFiled: April 15, 2014Publication date: December 18, 2014Applicant: IntegenX Inc.Inventors: Mattias Vangbo, William D. Nielsen, Iuliu Iouan Blaga, Seth Stern, Joanne Horn, Michael Van Nguyen, Stevan Jovanovich
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Patent number: 8763642Abstract: This invention provides a fluidic device comprising a diaphragm valve having a fluidics layer, an actuation layer and an elastic layer between the fluidics layer and the actuation layer, the elastic layer having a diaphragm that is mechanically sealed against the fluidics layer and the actuation layer by a sealing ring in the actuation layer.Type: GrantFiled: August 20, 2011Date of Patent: July 1, 2014Assignee: IntegenX Inc.Inventor: Mattias Vangbo
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Publication number: 20140170645Abstract: The invention provides a system that can process a raw biological sample, perform a biochemical reaction and provide an analysis readout. For example, the system can extract DNA from a swab, amplify STR loci from the DNA, and analyze the amplified loci and STR markers in the sample. The system integrates these functions by using microfluidic components to connect what can be macrofluidic functions. In one embodiment the system includes a sample purification module, a reaction module, a post-reaction clean-up module, a capillary electrophoresis module and a computer. In certain embodiments, the system includes a disposable cartridge for performing analyte capture. The cartridge can comprise a fluidic manifold having macrofluidic chambers mated with microfluidic chips that route the liquids between chambers. The system fits within an enclosure of no more than 10 ft3. and can be a closed, portable, and/or a battery operated system. The system can be used to go from raw sample to analysis in less than 4 hours.Type: ApplicationFiled: August 15, 2013Publication date: June 19, 2014Applicant: IntegenX Inc.Inventors: Stevan B. Jovanovich, William D. Nielsen, David S. Cohen, Michael Recknor, Mattias Vangbo, Ezra Van Gelder, Lars Majlof, Omar El-Sissi
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Patent number: 8748165Abstract: This invention provides a method for generating short tandem repeat (STR) profiles on each of a plurality of samples comprising, for each sample: a) isolating DNA from the sample; b) amplifying STR markers in the isolated DNA and c) analyzing the amplification product by electrophoresis.Type: GrantFiled: August 21, 2012Date of Patent: June 10, 2014Assignee: IntegenX Inc.Inventors: Mattias Vangbo, William D. Nielsen, Iuliu I. Blaga, Michael Van Nguyen, Steven B. Jovanovich
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Publication number: 20140045704Abstract: The present invention discloses the integration of programmable microfluidic circuits to achieve practical applications to process biochemical and chemical reactions and to integrate these reactions. In some embodiments workflows for biochemical reactions or chemical workflows are combined. Microvalves such as programmable microfluidic circuit with Y valves and flow through valves are disclosed. In some embodiments microvalves of the present invention are used for mixing fluids, which may be part of an integrated process. These processes include mixing samples and moving reactions to an edge or reservoir for modular microfluidics, use of capture regions, and injection into analytical devices on separate devices. In some embodiments star and nested star designs, or bead capture by change of cross sectional area of a channel in a microvalve are used. Movement of samples between temperature zones are further disclosed using fixed temperature and movement of the samples by micropumps.Type: ApplicationFiled: May 2, 2013Publication date: February 13, 2014Applicant: IntegenX Inc.Inventors: Stevan Bogdan Jovanovich, luliu I. Balga, Allen R. Boronkay, Joanne Horn, Michael Van Nguyen, William D. Nielsen, Mattias Vangbo
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Patent number: 8562918Abstract: The invention provides a system that can process a raw biological sample, perform a biochemical reaction and provide an analysis readout. For example, the system can extract DNA from a swab, amplify STR loci from the DNA, and analyze the amplified loci and STR markers in the sample. The system integrates these functions by using microfluidic components to connect what can be macrofluidic functions. In one embodiment the system includes a sample purification module, a reaction module, a post-reaction clean-up module, a capillary electrophoresis module and a computer. In certain embodiments, the system includes a disposable cartridge for performing analyte capture. The cartridge can comprise a fluidic manifold having macrofluidic chambers mated with microfluidic chips that route the liquids between chambers. The system fits within an enclosure of no more than 10 ft3. and can be a closed, portable, and/or a battery operated system. The system can be used to go from raw sample to analysis in less than 4 hours.Type: GrantFiled: December 17, 2012Date of Patent: October 22, 2013Assignee: IntegenX Inc.Inventors: Stevan B. Jovanovich, William D. Nielsen, David S. Cohen, Michael Recknor, Mattias Vangbo, Ezra Van Gelder, Lars Majlof, Omar El-Sissi