Abstract: The present invention relates to the long-term preservation of biological material. More specifically, it concerns a preservation container comprising: a biological container for containing biological material, a first shield configured for absorbing gamma-rays, a second shield configured for absorbing ambient neutrons, said second shield surrounding the biological container, the preservation container being of low-radioactivity background materials, and a method for preserving a biological material, comprising: a) providing a biological material in a confinement container, b) providing the preservation container of the invention, c) placing the confinement container containing the biological material into said preservation container, d) storing said preservation container containing the biological material in a room located under a material attenuating cosmic rays and induced particles, said material having a thickness equivalent to 1 m to 7000 m of water, for attenuating cosmic rays.