Patents by Inventor Mauricio Alvira

Mauricio Alvira has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • SCALABLE PURIFICATION METHOD FOR AAV9
    Publication number: 20230416695
    Abstract: A two-step chromatography purification scheme is described which selectively captures and isolates the genome-containing rAAV vector particles from the clarified, concentrated supernatant of a rAAV production cell culture. The process utilizes an affinity capture method performed at a high salt concentration followed by an anion exchange resin method performed at high pH to provide rAAV vector particles which are substantially free of rAAV intermediates.
    Type: Application
    Filed: June 27, 2023
    Publication date: December 28, 2023
    Inventors: Martin Lock, Mauricio Alvira
  • SCALABLE PURIFICATION METHOD FOR AAV1
    Publication number: 20230323312
    Abstract: A two-step chromatography purification scheme is described which selectively captures and isolates the genome-containing rAAV vector particles from the clarified, concentrated supernatant of a rAAV production cell culture. The process utilizes an affinity capture method performed at a high salt concentration followed by an anion exchange resin method performed at high pH to provide rAAV vector particles which are substantially free of rAAV intermediates.
    Type: Application
    Filed: June 13, 2023
    Publication date: October 12, 2023
    Applicant: The Trustees of the University of Pennsylvania
    Inventors: Martin Lock, Mauricio Alvira
  • Scalable purification method for AAV9
    Patent number: 11732245
    Abstract: A two-step chromatography purification scheme is described which selectively captures and isolates the genome-containing rAAV vector particles from the clarified, concentrated supernatant of a rAAV production cell culture. The process utilizes an affinity capture method performed at a high salt concentration followed by an anion exchange resin method performed at high pH to provide rAAV vector particles which are substantially free of rAAV intermediates.
    Type: Grant
    Filed: July 19, 2021
    Date of Patent: August 22, 2023
    Assignee: The Trustees of the University of Pennsylvania
    Inventors: Martin Lock, Mauricio Alvira
  • Scalable purification method for AAV8
    Patent number: 11718835
    Abstract: A two-step chromatography purification scheme is described which selectively captures and isolates the genome-containing rAAV vector particles from the clarified, concentrated supernatant of a rAAV production cell culture. The process utilizes an affinity capture method performed at a high salt concentration followed by an anion exchange resin method performed at high pH to provide rAAV vector particles which are substantially free of rAAV intermediates.
    Type: Grant
    Filed: May 24, 2021
    Date of Patent: August 8, 2023
    Assignee: Ths Trustees of the University of Pennsylvania
    Inventors: Martin Lock, Mauricio Alvira
  • Scalable purification method for AAV1
    Patent number: 11713450
    Abstract: A two-step chromatography purification scheme is described which selectively captures and isolates the genome-containing rAAV vector particles from the clarified, concentrated supernatant of a rAAV production cell culture. The process utilizes an affinity capture method performed at a high salt concentration followed by an anion exchange resin method performed at high pH to provide rAAV vector particles which are substantially free of rAAV intermediates.
    Type: Grant
    Filed: May 24, 2021
    Date of Patent: August 1, 2023
    Assignee: Ths Trustees of the University of Pennsylvania
    Inventors: Martin Lock, Mauricio Alvira
  • Scalable purification method for AAVRH10
    Patent number: 11713449
    Abstract: A two-step chromatography purification scheme is described which selectively captures and isolates the genome-containing rAAV vector particles from the clarified, concentrated supernatant of a rAAV production cell culture. The process utilizes an affinity capture method performed at a high salt concentration followed by an anion exchange resin method performed at high pH to provide rAAV vector particles which are substantially free of rAAV intermediates.
    Type: Grant
    Filed: April 21, 2021
    Date of Patent: August 1, 2023
    Assignee: Ths Trustees of the University of Pennsylvania
    Inventors: Martin Lock, Mauricio Alvira
  • SCALABLE PURIFICATION METHOD FOR AAV9
    Publication number: 20210348133
    Abstract: A two-step chromatography purification scheme is described which selectively captures and isolates the genome-containing rAAV vector particles from the clarified, concentrated supernatant of a rAAV production cell culture. The process utilizes an affinity capture method performed at a high salt concentration followed by an anion exchange resin method performed at high pH to provide rAAV vector particles which are substantially free of rAAV intermediates.
    Type: Application
    Filed: July 19, 2021
    Publication date: November 11, 2021
    Inventors: Martin Lock, Mauricio Alvira
  • SCALABLE PURIFICATION METHOD FOR AAV1
    Publication number: 20210348132
    Abstract: A two-step chromatography purification scheme is described which selectively captures and isolates the genome-containing rAAV vector particles from the clarified, concentrated supernatant of a rAAV production cell culture. The process utilizes an affinity capture method performed at a high salt concentration followed by an anion exchange resin method performed at high pH to provide rAAV vector particles which are substantially free of rAAV intermediates.
    Type: Application
    Filed: May 24, 2021
    Publication date: November 11, 2021
    Inventors: Martin Lock, Mauricio Alvira
  • SCALABLE PURIFICATION METHOD FOR AAV8
    Publication number: 20210277364
    Abstract: A two-step chromatography purification scheme is described which selectively captures and isolates the genome-containing rAAV vector particles from the clarified, concentrated supernatant of a rAAV production cell culture. The process utilizes an affinity capture method performed at a high salt concentration followed by an anion exchange resin method performed at high pH to provide rAAV vector particles which are substantially free of rAAV intermediates.
    Type: Application
    Filed: May 24, 2021
    Publication date: September 9, 2021
    Inventors: Martin Lock, Mauricio Alvira
  • Scalable purification method for AAV9
    Patent number: 11098286
    Abstract: A two-step chromatography purification scheme is described which selectively captures and isolates the genome-containing rAAV vector particles from the clarified, concentrated supernatant of a rAAV production cell culture. The process utilizes an affinity capture method performed at a high salt concentration followed by an anion exchange resin method performed at high pH to provide rAAV vector particles which are substantially free of rAAV intermediates.
    Type: Grant
    Filed: December 9, 2016
    Date of Patent: August 24, 2021
    Assignee: The Trustees of the University of Pennsylvania
    Inventors: Martin Lock, Mauricio Alvira
  • SCALABLE PURIFICATION METHOD FOR AAVRH10
    Publication number: 20210238560
    Abstract: A two-step chromatography purification scheme is described which selectively captures and isolates the genome-containing rAAV vector particles from the clarified, concentrated supernatant of a rAAV production cell culture. The process utilizes an affinity capture method performed at a high salt concentration followed by an anion exchange resin method performed at high pH to provide rAAV vector particles which are substantially free of rAAV intermediates.
    Type: Application
    Filed: April 21, 2021
    Publication date: August 5, 2021
    Inventors: Martin Lock, Mauricio Alvira
  • Scalable purification method for AAVRH10
    Patent number: 11028372
    Abstract: A two-step chromatography purification scheme is described which selectively captures and isolates the genome-containing rAAV vector particles from the clarified, concentrated supernatant of a rAAV production cell culture. The process utilizes an affinity capture method performed at a high salt concentration followed by an anion exchange resin method performed at high pH to provide rAAV vector particles which are substantially free of rAAV intermediates.
    Type: Grant
    Filed: December 9, 2016
    Date of Patent: June 8, 2021
    Assignee: The Trustees of the University of Pennsylvania
    Inventors: Martin Lock, Mauricio Alvira
  • Scalable purification method for AAV1
    Patent number: 11015173
    Abstract: A two-step chromatography purification scheme is described which selectively captures and isolates the genome-containing rAAV vector particles from the clarified, concentrated supernatant of a rAAV production cell culture. The process utilizes an affinity capture method performed at a high salt concentration followed by an anion exchange resin method performed at high pH to provide rAAV vector particles which are substantially free of rAAV intermediates.
    Type: Grant
    Filed: December 9, 2016
    Date of Patent: May 25, 2021
    Assignee: The Trustees of the University of Pennsylvania
    Inventors: Martin Lock, Mauricio Alvira
  • Scalable purification method for AAV8
    Patent number: 11015174
    Abstract: A two-step chromatography purification scheme is described which selectively captures and isolates the genome-containing rAAV vector particles from the clarified, concentrated supernatant of a rAAV production cell culture. The process utilizes an affinity capture method performed at a high salt concentration followed by an anion exchange resin method performed at high pH to provide rAAV vector particles which are substantially free of rAAV intermediates.
    Type: Grant
    Filed: December 9, 2016
    Date of Patent: May 25, 2021
    Assignee: The Trustees of the University of Pennsylvania
    Inventors: Martin Lock, Mauricio Alvira
  • SCALABLE PURIFICATION METHOD FOR AAV1
    Publication number: 20190002843
    Abstract: A two-step chromatography purification scheme is described which selectively captures and isolates the genome-containing rAAV vector particles from the clarified, concentrated supernatant of a rAAV production cell culture. The process utilizes an affinity capture method performed at a high salt concentration followed by an anion exchange resin method performed at high pH to provide rAAV vector particles which are substantially free of rAAV intermediates.
    Type: Application
    Filed: December 9, 2016
    Publication date: January 3, 2019
    Inventors: Martin Lock, Mauricio Alvira
  • SCALABLE PURIFICATION METHOD FOR AAVRH10
    Publication number: 20190002841
    Abstract: A two-step chromatography purification scheme is described which selectively captures and isolates the genome-containing rAAV vector particles from the clarified, concentrated supernatant of a rAAV production cell culture.
    Type: Application
    Filed: December 9, 2016
    Publication date: January 3, 2019
    Inventors: Martin Lock, Mauricio Alvira
  • SCALABLE PURIFICATION METHOD FOR AAV9
    Publication number: 20190002842
    Abstract: A two-step chromatography purification scheme is described which selectively captures and isolates the genome-containing rAAV vector particles from the clarified, concentrated supernatant of a rAAV production cell culture. The process utilizes an affinity capture method performed at a high salt concentration followed by an anion exchange resin method performed at high pH to provide rAAV vector particles which are substantially free of rAAV intermediates.
    Type: Application
    Filed: December 9, 2016
    Publication date: January 3, 2019
    Inventors: Martin Lock, Mauricio Alvira
  • SCALABLE PURIFICATION METHOD FOR AAV8
    Publication number: 20190002844
    Abstract: A two-step chromatography purification scheme is described which selectively captures and isolates the genome-containing rAAV vector particles from the clarified, concentrated supernatant of a rAAV production cell culture. The process utilizes an affinity capture method performed at a high salt concentration followed by an anion exchange resin method performed at high pH to provide rAAV vector particles which are substantially free of rAAV intermediates.
    Type: Application
    Filed: December 9, 2016
    Publication date: January 3, 2019
    Inventors: Martin Lock, Mauricio Alvira
  • Adeno-associated virus (AAV) serotype 8 sequences, vectors containing same, and uses therefor
    Publication number: 20080075740
    Abstract: Sequences of a serotype 8 adeno-associated virus and vectors and host cells containing these sequences are provided. Also described are methods of using such host cells and vectors in production of rAAV particles.
    Type: Application
    Filed: October 31, 2007
    Publication date: March 27, 2008
    Applicant: The Trustees of the University of Pennsylvania
    Inventors: Guangping Gao, James Wilson, Mauricio Alvira
  • Adeno-Associated Virus (AAV) serotype 8 sequences, vectors containing same, and uses therefor
    Publication number: 20080075737
    Abstract: Sequences of a serotype 8 adeno-associated virus and vectors and host cells containing these sequences are provided. Also described are methods of using such host cells and vectors in production of rAAV particles. rAAV8-mediated delivery of therapeutic and immunogenic genes, including Factor VIII, is also provided.
    Type: Application
    Filed: September 6, 2007
    Publication date: March 27, 2008
    Applicant: The Trustees of the University of Pennsylvania
    Inventors: Guangping Gao, James Wilson, Mauricio Alvira