Patents by Inventor Maurine E. Linder

Maurine E. Linder has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • HIGH-THROUGHPUT ENZYME ASSAY FOR SCREENING ACTIVITY OF ACYLTRANSFERASES
    Publication number: 20220403443
    Abstract: A method for determining activity of an acyl transferase enzyme, the method comprising: (i) preparing a reaction mixture comprising: (a) an acyl transferase enzyme, (b) a peptide substrate bound to a fluorophore, wherein the substrate is a cysteine-containing oligopeptide of 5-25 amino acids in length, (c) an acyl-CoA, and (d) a detergent comprising micelles, wherein the acyl transferase enzyme mediates acylation on a cysteine of said peptide substrate to result in association of the peptide with micelles of the detergent with resultant increase in fluorescence polarization; and (ii) measuring fluorescent signal of the reaction mixture; wherein an increase in fluorescence polarization of the reaction mixture compared to fluorescence polarization of a control reaction indicates acyl transferase activity of the acyl transferase enzyme. The above assay method may also be used for screening compounds for their ability to act as inhibitors of an acyl transferase enzyme.
    Type: Application
    Filed: June 14, 2022
    Publication date: December 22, 2022
    Inventors: Hening Lin, Maurine E. Linder, Jun Young Hong
  • Human protein acyl transferases and methods of uses therefor
    Patent number: 7883860
    Abstract: The present invention provides the identification of human Ras palmitoyl acyl transfersase complexes, and nucleic acids coding therefor. In addition, methods of screening for modulators of human Ras palmitoyl acyl transfersase, including high throughput yeast screens, are also provided.
    Type: Grant
    Filed: December 30, 2008
    Date of Patent: February 8, 2011
    Assignees: Washington University, The University of Iowa Research Foundation
    Inventors: Robert J. Deschenes, Sandra Lobo, John Swarthout, Maurine E. Linder
  • HUMAN PROTEIN ACYL TRANSFERASES AND METHODS OF USES THEREFOR
    Publication number: 20090208990
    Abstract: The present invention provides the identification of human Ras palmitoyl acyl transfersase complexes, and nucleic acids coding therefor. In addition, methods of screening for modulators of human Ras palmitoyl acyl transfersase, including high throughput yeast screens, are also provided.
    Type: Application
    Filed: December 30, 2008
    Publication date: August 20, 2009
    Applicants: Washington University St. Louis, University of lowa Research Foundation
    Inventors: Robert J. Deschenes, Sandra Lobo, John Swarthout, Maurine E. Linder
  • Human protein acyl transferases and methods of uses therefor
    Patent number: 7488592
    Abstract: The present invention provides the identification of human Ras palmitoyl acyl transfersase complexes, and nucleic acids coding therefore. In addition, methods of screening for modulators of human Ras palmitoyl acyl transfersase, including high throughput yeast screens, are also provided.
    Type: Grant
    Filed: September 22, 2004
    Date of Patent: February 10, 2009
    Assignees: Washington University, The University of Iowa Research Foundation
    Inventors: Robert J. Deschenes, Sandra Lobo, John Swarthout, Maurine E. Linder